{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:35:48Z","timestamp":1791524148799,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012320","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000}}],"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T00:00:00Z","timestamp":1721001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013041","name":"Vice-Chancellor for Research, Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["18475"],"award-info":[{"award-number":["18475"]}],"id":[{"id":"10.13039\/501100013041","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"\nBackground<\/jats:title>\nThe sensitivity of parasitological and molecular methods is unsatisfactory for the diagnosis of strongyloidiasis, and serological techniques are remaining as the most effective diagnostic approach. The present study aimed to design and produce a chimeric recombinant antigen from Strongyloides stercoralis<\/jats:italic> immunoreactive antigen (SsIR<\/jats:italic>) and Ss1a antigens, using immune-informatics approaches, and evaluated its diagnostic performance in an ELISA system for the diagnosis of human strongyloidiasis.<\/jats:p>\n<\/jats:sec>\n\nMethodology\/Principal findings<\/jats:title>\nThe coding sequences for SsIR<\/jats:italic> and Ss1a were selected from GenBank and were gene-optimized. Using bioinformatics analysis, the regions with the highest antigenicity that did not overlap with other parasite antigens were selected. The chimeric recombinant antigen SsIR- Ss1a, was constructed. The solubility and physicochemical properties of the designed construct were analyzed and its tertiary structures were built and evaluated. The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E<\/jats:italic>. coli<\/jats:italic> DH5\u03b1 cells. Diagnostic performances of the produced recombinant antigen, along with a commercial kit were evaluated in an indirect ELISA system, using a panel of sera from strongyloidiasis patients and controls.<\/jats:p>\nThe physicochemical and bioinformatics evaluations revealed that the designed chimeric construct is soluble, has a molecular with of 35 KDa, and is antigenic. Western blotting confirmed the immunoreactivity of the produced chimeric recombinant antigen with the sera of strongyloidiasis patients. The sensitivity and specificity of the indirect ELISA system, using the produced SsIR-Ss1a chimeric antigen, were found to be 93.94% (95% CI, 0.803 to 0.989) and 97.22% (95% CI, 0.921 to 0.992) respectively.<\/jats:p>\n<\/jats:sec>\n\nConclusions\/Significance<\/jats:title>\nThe preliminary findings of this study suggest that the produced SsIR-Ss1a chimeric antigen shows promise in the diagnosis of human strongyloidiasis. However, these results are based on a limited panel of samples, and further research with a larger sample size is necessary to confirm its accuracy. The construct has potential as an antigen in the ELISA system for the serological diagnosis of this neglected parasitic infection, but additional validation is required.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012320","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T17:45:54Z","timestamp":1721065554000},"page":"e0012320","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity"],"prefix":"10.1371","volume":"18","author":[{"given":"Mostafa","family":"Omidian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh","family":"Mostafavi-Pour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzieh","family":"Asadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meysam","family":"Sharifdini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navid","family":"Nezafat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Pouryousef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Savardashtaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mortaza","family":"Taheri-Anganeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fattaneh","family":"Mikaeili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2045-9057","authenticated-orcid":true,"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"issue":"6","key":"pntd.0012320.ref001","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3390\/pathogens9060468","article-title":"The global prevalence of Strongyloides stercoralis infection.","volume":"9","author":"D Buonfrate","year":"2020","journal-title":"Pathogens"},{"issue":"4","key":"pntd.0012320.ref002","doi-asserted-by":"crossref","first-page":"e0008184","DOI":"10.1371\/journal.pntd.0008184","article-title":"The Strongyloides stercoralis-hookworms association as a path to the estimation of the global burden of strongyloidiasis: A systematic review.","volume":"14","author":"PE Fleitas","year":"2020","journal-title":"PLOS Neglected Tropical Diseases"},{"issue":"12","key":"pntd.0012320.ref003","doi-asserted-by":"crossref","first-page":"e0005253","DOI":"10.1371\/journal.pntd.0005253","article-title":"Genome-wide analyses of individual Strongyloides stercoralis (Nematoda: Rhabditoidea) provide insights into population structure and reproductive life cycles.","volume":"10","author":"T Kikuchi","year":"2016","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"pntd.0012320.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-0500-6-200","article-title":"Strongyloides stercoralis is a cause of abdominal pain, diarrhea and urticaria in rural Cambodia.","volume":"6","author":"V Khieu","year":"2013","journal-title":"BMC research notes."},{"key":"pntd.0012320.ref005","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s15010-015-0799-1","article-title":"Strongyloides stercoralis hyperinfection syndrome: a case series and a review of the literature.","volume":"43","author":"G Geri","year":"2015","journal-title":"Infection"},{"key":"pntd.0012320.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.apar.2022.07.001","article-title":"The diagnosis of human and companion animal Strongyloides stercoralis infection: challenges and solutions. 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