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Currently, the commercial test kits are poor at assessing <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic> infection and vaccine effectiveness, making an urgent need to exploit effective enzyme-linked immunosorbent assay with great performance to compensate for this deficiency. Here, the TgIMP1 recombinant protein was expressed in <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> BL(21) cells. The TgIMP1 was purified with affinity chromatography and the reactivity was retained with anti-TgIMP1 antibodies. The TgIMP1 was then used to develop an indirect ELISA (IMP1-iELISA) and the reaction conditions of IMP1-iELISA were optimized. As a result, the cut-off value was determined to be 0.2833 by analyzing the OD<jats:sub>450nm<\/jats:sub> values of forty <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>-negative sera. The coefficient of variation of 6 <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>-positive sera within and between runs were both less than 10%. The IMP1-iELISA was non-cross-reactive with the sera of cytomegalovirus, herpes virus, rubella virus, <jats:italic>Cryptosporidium<\/jats:italic> spp., <jats:italic>Theileria<\/jats:italic> spp., <jats:italic>Neospora<\/jats:italic> spp. and <jats:italic>Plasmodium<\/jats:italic> spp.. Furthermore, the sensitivity and specificity of IMP1-iELISA were 98.9% and 96.7%, respectively, based on testing 150 serum samples. The results suggest that this IMP1-iELISA is specific, sensitive, repeatable and can be applied to the detection of T. gondii infections in the medical and health industries.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012421","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T17:33:41Z","timestamp":1723656821000},"page":"e0012421","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development of an indirect ELISA for detecting Toxoplasma gondii IgG antibodies based on a recombinant TgIMP1 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