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In\n                    <jats:italic>Paracoccidioides<\/jats:italic>\n                    species, previous studies have demonstrated the upregulation of this enzyme in a wide range of starvation and infective-like conditions. However,\n                    <jats:italic>Paracoccidioides lutzii<\/jats:italic>\n                    formamidase has not yet been defined as a virulence factor. Here, by employing\n                    <jats:italic>in vivo<\/jats:italic>\n                    infections using an\n                    <jats:italic>fmd<\/jats:italic>\n                    -silenced strain in\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    larvae model, we demonstrate the influence of formamidase in\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>lutzii<\/jats:italic>\n                    \u2019s immune stimulation and pathogenicity. The formamidase silencing resulted in improper arrangement of the nodules, poor melanogenesis and decreased fungal burden. Thus, we suggest that formamidase may be a piece composing the process of molecular recognition by\n                    <jats:italic>Galleria<\/jats:italic>\n                    immune cells. Furthermore, formamidase silencing doubled the observed survival rate of the larvae, demonstrating its importance in fungal virulence\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Therefore, our findings indicate that formamidase contributes to\n                    <jats:italic>Galleria<\/jats:italic>\n                    \u2019s immune incitement and establishes the role of this enzyme as a\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>lutzii<\/jats:italic>\n                    virulence factor.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0012452","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T13:37:16Z","timestamp":1725370636000},"page":"e0012452","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor"],"prefix":"10.1371","volume":"18","author":[{"given":"Elisa Dias","family":"Pereira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalison Rodrigues","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Rafaela Milhomem","family":"Cruz-Leite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Vieira","family":"Tomazett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lana O\u2019Hara","family":"Souza Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Graziani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Assis","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Corr\u00eaa","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Schneider","family":"Weber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Alves","family":"Parente-Rocha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Maria de Almeida","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9914-8771","authenticated-orcid":true,"given":"Clayton Luiz","family":"Borges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,9,3]]},"reference":[{"issue":"2","key":"pntd.0012452.ref001","first-page":"89","article-title":"Paracoccidioidomycosis: an Update.","volume":"6","author":"E Brummer","year":"1993"},{"issue":"5","key":"pntd.0012452.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mSphere.00213-16","article-title":"CA. 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