{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:45:48Z","timestamp":1791935148495,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012523","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T00:00:00Z","timestamp":1728345600000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372263"],"award-info":[{"award-number":["31372263"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI182096"],"award-info":[{"award-number":["R01AI182096"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    The spread and invasion of the urban malaria vector\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    has emerged as a significant threat to ongoing malaria control and elimination efforts, particularly in Africa. The successful use of the maternally inherited endosymbiotic bacterium\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    for arbovirus control has inspired the exploration of similar strategies for managing malaria vectors, necessitating the establishment of a stable\n                    <jats:italic>Wolbachia-Anopheles<\/jats:italic>\n                    symbiosis. In this study, we successfully transferred\n                    <jats:italic>Wolbachia w<\/jats:italic>\n                    Pip into\n                    <jats:italic>An<\/jats:italic>\n                    .\n                    <jats:italic>stephensi<\/jats:italic>\n                    , resulting in the establishment of a stable transinfected HP1 line with 100% maternal transmission efficiency. We demonstrate that\n                    <jats:italic>w<\/jats:italic>\n                    Pip in the HP1 line induces nearly complete unidirectional cytoplasmic incompatibility (CI) and maintains high densities in both somatic and germline tissues. Despite a modest reduction in lifespan and female reproductive capacity, our results suggest the\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infection in the HP1 line has little impact on life history traits, body size, and male mating competitiveness, as well as the ability of its larvae to tolerate rearing temperatures up to 38\u00b0C, although\n                    <jats:italic>w<\/jats:italic>\n                    Pip densities moderately decrease when larvae are exposed to a constant 33\u00b0C and diurnal cyclic temperatures of 27\u201336\u00b0C and 27\u201338\u00b0C. These findings highlight the potential of the HP1 line as a robust candidate for further development in malaria control.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0012523","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T13:46:18Z","timestamp":1727358378000},"page":"e0012523","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Stable introduction of Wolbachia wPip into invasive Anopheles stephensi for potential malaria control"],"prefix":"10.1371","volume":"18","author":[{"given":"Yongkang","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YiLian","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6609-5829","authenticated-orcid":true,"given":"Yu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-012X","authenticated-orcid":true,"given":"Zhiyong","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"issue":"3","key":"pntd.0012523.ref001","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.cell.2016.07.055","article-title":"Malaria: Biology and Disease.","volume":"167","author":"AF Cowman","year":"2016","journal-title":"Cell"},{"issue":"6","key":"pntd.0012523.ref002","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S1473-3099(04)01043-6","article-title":"The global distribution and population at risk of malaria: past, present, and future","volume":"4","author":"SI Hay","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"pntd.0012523.ref003","unstructured":"WHO. 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