{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T02:28:57Z","timestamp":1772850537731,"version":"3.50.1"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2020-119035RB-100"],"award-info":[{"award-number":["PID2020-119035RB-100"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"EDCTP2","award":["RIA2017NCT-1845-STOP"],"award-info":[{"award-number":["RIA2017NCT-1845-STOP"]}]},{"name":"European Union Next Generation"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Strongyloidiasis is a neglected tropical disease estimated to affect more than 600 million people worldwide. Recently, the World Health Organization road map on neglected tropical diseases 2021\u20132030 has put the focus on strongyloidiasis, including this disease within its mass drug administration campaigns. With the use of ivermectin in extensive treatment of all populations at-risk, identifying effective therapeutic alternatives is crucial in case ivermectin resistance arises. The objective of the present study was the development of a larval migration inhibition assay to evaluate the anthelmintic efficacy of commercial drugs and diamine and aminoalcohol derivatives against infective <jats:italic>Strongyloides ratti<\/jats:italic> third stage larvae. Through this technique, we successfully screened and estimated the <jats:italic>in vitro<\/jats:italic> anthelmintic efficacy of six commercial drugs, seven diamine derivatives and eight aminoalcohol derivatives. Unexpectedly, the half-maximal effective concentration of ivermectin and moxidectin (2.21 and 2.34 \u03bcM, respectively) were observed as the highest value obtained among all commercial drugs tested by this <jats:italic>in vitro<\/jats:italic> technique. Moreover, some diamine and aminoalcohol derivatives showed superior efficacy inhibiting <jats:italic>S<\/jats:italic>. <jats:italic>ratti<\/jats:italic> motility compared to ivermectin, with five compounds (AA23, AA34, AO2 AO7 and AO14b) also displaying selectivity indexes on HepG2 and Caco2 higher than 1. These findings underscore the potential of these derivatives as promising alternatives for strongyloidiasis treatment, warranting further investigation and <jats:italic>in vivo<\/jats:italic> efficacy assessment.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012532","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T17:21:10Z","timestamp":1728321670000},"page":"e0012532","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating alternative compounds for strongyloidiasis therapy: Novel insights from larval migration inhibition test"],"prefix":"10.1371","volume":"18","author":[{"given":"Mar\u00eda","family":"Cambra-Pellej\u00e0","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elora","family":"Valderas-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Bala\u00f1a-Fouce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"de la Vega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"del Olmo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Antwi-Ekwuruke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Linnemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lennart","family":"Heepmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minka","family":"Breloer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3723-1895","authenticated-orcid":true,"given":"Mar\u00eda","family":"Mart\u00ednez-Valladares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"issue":"6","key":"pntd.0012532.ref001","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3390\/pathogens9060468","article-title":"The Global Prevalence of Strongyloides stercoralis Infection","volume":"9","author":"D Buonfrate","year":"2020","journal-title":"Pathogens"},{"issue":"6","key":"pntd.0012532.ref002","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.4269\/ajtmh.18-0895","article-title":"Morbidity Associated with Chronic Strongyloides stercoralis Infection: A Systematic Review and Meta-Analysis","volume":"100","author":"F Tamarozzi","year":"2019","journal-title":"Am J Trop Med Hyg"},{"issue":"10","key":"pntd.0012532.ref003","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1093\/trstmh\/trad032","article-title":"Severe strongyloidiasis: a systematic review and meta-analysis of 339 cases","volume":"117","author":"OC Rojas","year":"2023","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"1894","key":"pntd.0012532.ref004","doi-asserted-by":"crossref","first-page":"20220440","DOI":"10.1098\/rstb.2022.0440","article-title":"Strongyloides ratti infection in mice: immune response and immune modulation","volume":"379","author":"M Breloer","year":"2024","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"3","key":"pntd.0012532.ref005","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1017\/S0031182016000111","article-title":"Strongyloides infection in rodents: immune response and immune regulation","volume":"144","author":"M Breloer","year":"2017","journal-title":"Parasitology"},{"key":"pntd.0012532.ref006","doi-asserted-by":"crossref","first-page":"e013439","DOI":"10.1136\/bmjgh-2023-013439","article-title":"Mass drug administration for neglected tropical disease control and elimination: a systematic review of ethical reasons","volume":"9","author":"J Hoefle-B\u00e9nard","year":"2024","journal-title":"BMJ Glob Health"},{"key":"pntd.0012532.ref007","unstructured":"World Health Organization, WHO. 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