{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:26:22Z","timestamp":1780392382334,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012645","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000}}],"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","award":["LHGJ20200372"],"award-info":[{"award-number":["LHGJ20200372"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Inflammatory bowel disease (IBD) increases the risk of colorectal cancer, and it has the potential to diminish the quality of life. Clinical and experimental evidence demonstrate protective aspects of parasitic helminth infection against IBD. However, studies on the inhibition of inflammation by helminth infection have overlooked a key determinant of health: the gut microbiota. Although infection with helminths induces alterations in the host microbiota composition, the potential influence and mechanism of helminth infections induced changes in the gut microbiota on the development of IBD has not yet been elucidated. In this study, we analyzed the intersection of helminth <jats:italic>Trichinella spiralis<\/jats:italic> and gut bacteria in the regulation of colitis and related mechanisms.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p><jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> infected mice were treated with antibiotics or cohoused with wild type mice, then challenged with dextran sodium sulfate (DSS)-colitis and disease severity, immune responses and goblet cells assessed. Gut bacteria composition was assessed by 16S rRNA sequencing and short-chain fatty acids (SCFAs) were measured. We found that protection against disease by infection with <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> was abrogated by antibiotic treatment, and cohousing with <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>- infected mice suppressed DSS-colitis in wild type mice. Bacterial community profiling revealed an increase in the abundance of the bacterial genus <jats:italic>Muribaculum<\/jats:italic> and <jats:italic>unclassified_Muribaculaceae<\/jats:italic> in mice with <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> infection or mice cohoused with <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>- infected mice. Metabolomic analysis demonstrated significantly increased propionic acid in feces from <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>- infected mice. Data also showed that the gut microbiome modulated by <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> exhibited enhanced goblet cell differentiation and elevated IL-10 levels in mice.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>These findings identify the gut microbiome as a critical component of the anti- colitic effect of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> and gives beneficial insights into the processes by which helminth alleviates colitis.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012645","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T18:39:05Z","timestamp":1730745545000},"page":"e0012645","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["The gut microbiota is essential for Trichinella spiralis\u2014evoked suppression of colitis"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4617-5474","authenticated-orcid":true,"given":"Hualei","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao Rong","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Ran","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing Jing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi Xuan","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"issue":"8","key":"pntd.0012645.ref001","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1038\/s41590-019-0415-0","article-title":"Targeting immune cell circuits and trafficking in inflammatory bowel disease","volume":"20","author":"MF Neurath","year":"2019","journal-title":"Nat Immunol"},{"key":"pntd.0012645.ref002","doi-asserted-by":"crossref","first-page":"111320","DOI":"10.1016\/j.intimp.2023.111320","article-title":"Trichinella-derived protein ameliorates colitis by altering the gut microbiome and improving intestinal barrier function","volume":"127","author":"SR Long","year":"2024","journal-title":"Int Immunopharmacol"},{"issue":"8","key":"pntd.0012645.ref003","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s11894-019-0705-6","article-title":"Evolving epidemiology of IBD.","volume":"21","author":"JW Windsor","year":"2019","journal-title":"Curr Gastroenterol Rep"},{"issue":"12","key":"pntd.0012645.ref004","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1038\/nrgastro.2015.150","article-title":"The global burden of IBD: from 2015 to 2025.","volume":"12","author":"GG Kaplan","year":"2015","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"3","key":"pntd.0012645.ref005","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1097\/MIB.0b013e31827f27f4","article-title":"Childhood helminth exposure is protective against inflammatory bowel disease: a case control study in South Africa.","volume":"19","author":"KM Chu","year":"2013","journal-title":"Inflamm Bowel Dis"},{"issue":"1","key":"pntd.0012645.ref006","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1007\/s10620-010-1237-y","article-title":"Schistosoma mansoni infection but not egg antigen promotes recovery from colitis in outbred NMRI mice","volume":"56","author":"P Bodammer","year":"2011","journal-title":"Digestive diseases and sciences"},{"issue":"4","key":"pntd.0012645.ref007","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.4049\/jimmunol.1201457","article-title":"Heligmosomoides polygyrus bakeri infection activates colonic Foxp3+ T cells enhancing their capacity to prevent colitis","volume":"191","author":"L Hang","year":"2013","journal-title":"J Immunol"},{"issue":"4","key":"pntd.0012645.ref008","doi-asserted-by":"crossref","first-page":"e1005481","DOI":"10.1371\/journal.ppat.1005481","article-title":"IL-22 restrains tapeworm-mediated protection against experimental colitis via regulation of IL-25 expression.","volume":"12","author":"JL Reyes","year":"2016","journal-title":"PLoS Pathog"},{"issue":"10","key":"pntd.0012645.ref009","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nrgastro.2012.152","article-title":"The gut microbiota","volume":"9","author":"C Manichanh","year":"2012","journal-title":"IBD. 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