{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:11:04Z","timestamp":1791231064953,"version":"4.1.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-44-00048"],"award-info":[{"award-number":["24-44-00048"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Epidemiologically important food-borne trematodes <jats:italic>Opisthorchis viverrini<\/jats:italic> and <jats:italic>Clonorchis sinensis<\/jats:italic> are recognized as biological carcinogens of Group 1A, while <jats:italic>Opisthorchis felineus<\/jats:italic> is in Group 3 as noncarcinogenic to humans. Mechanisms of the biological carcinogenesis are still elusive. Some studies highlight chronic inflammation as a key factor and common pathway for cancer initiation and progression. Nonetheless, the chronic inflammation alone does not explain why these three species differ in carcinogenicity. We focused this study on genome-wide landscapes of liver gene expression and activation of cellular pathways in <jats:italic>Mesocricetus auratus<\/jats:italic> golden hamsters infected with <jats:italic>C<\/jats:italic>. <jats:italic>sinensis<\/jats:italic> (South Korea), <jats:italic>O<\/jats:italic>. <jats:italic>viverrini<\/jats:italic> (Thailand), or <jats:italic>O<\/jats:italic>. <jats:italic>felineus<\/jats:italic> (Russia) at 1 and 3 months after infection initiation.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>Liver transcriptomes of golden hamsters (HiSeq Illumina, 2X150 bp) were sequenced at 1 and 3 months postinfection. Data processing was carried out using the following bioinformatic and experimental approaches: analysis of differential gene expression, estimates of proportions of affected liver cell types, liver histopathology, and examination of weighted gene coexpression networks. All infections caused enrichment with inflammatory response signaling pathways, fibrogenesis and cell proliferation, and IL2\u2013STAT5, TNF\u2013NF-\u03baB, TGF-\u03b2, Hippo, MAPK, and PI3K\u2013Akt signaling pathways. Nevertheless, species-specific responses to each infection were noted too. We also identified species-specific responses of liver cell types, differentially expressed gene clusters, and cellular pathways associated with structural liver damage (such as periductal fibrosis, epithelial neoplasia, and inflammation).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>This is the first comparative analysis of gene expression landscapes in the liver of experimental animals infected with <jats:italic>O<\/jats:italic>. <jats:italic>viverrini<\/jats:italic>, <jats:italic>O<\/jats:italic>. <jats:italic>felineus<\/jats:italic>, or <jats:italic>C<\/jats:italic>. <jats:italic>sinensis<\/jats:italic>. The trematodes have species-specific effects on the hepatobiliary system by triggering signaling pathways, thereby leading to differences in the severity of hepatobiliary structural lesions and contributing to the pathogenicity of closely related foodborne trematodes.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012685","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T18:22:15Z","timestamp":1733768535000},"page":"e0012685","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative liver transcriptome analysis in hamsters infected with food-borne trematodes Opisthorchis felineus, Opisthorchis viverrini, or Clonorchis sinensis"],"prefix":"10.1371","volume":"18","author":[{"given":"Ekaterina A.","family":"Lishai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oxana G.","family":"Zaparina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaroslav K.","family":"Kapushchak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banchob","family":"Sripa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun-Jong","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofeng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-2068","authenticated-orcid":true,"given":"Maria Y.","family":"Pakharukova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,9]]},"reference":[{"issue":"1","key":"pntd.0012685.ref001","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1093\/trstmh\/trv085","article-title":"The liver fluke Opisthorchis felineus: biology, epidemiology and carcinogenic potential","volume":"110","author":"MY Pakharukova","year":"2016","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"7","key":"pntd.0012685.ref002","doi-asserted-by":"crossref","first-page":"e0132673","DOI":"10.1371\/journal.pone.0132673","article-title":"Association between liver fluke infection and hepatobiliary pathological changes: A systematic review and meta-analysis","volume":"10","author":"J Xia","year":"2015","journal-title":"PLoS One"},{"issue":"5","key":"pntd.0012685.ref003","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1007\/s00436-009-1548-0","article-title":"Alterations of gene expression of RB pathway in Opisthorchis viverrini infection-induced cholangiocarcinoma","volume":"105","author":"T Boonmars","year":"2009","journal-title":"Parasitol Res"},{"issue":"6","key":"pntd.0012685.ref004","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/j.jhep.2005.11.040","article-title":"Cholangiocarcinoma and Clonorchis sinensis infection: a case-control study in Korea","volume":"44","author":"D Choi","year":"2006","journal-title":"J Hepatol"},{"issue":"3","key":"pntd.0012685.ref005","doi-asserted-by":"crossref","first-page":"320","DOI":"10.6004\/jadpro.2022.13.3.28","article-title":"Updates in Cholangiocarcinoma","volume":"13","author":"K. 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