{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T23:22:44Z","timestamp":1783725764554,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012701","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000}}],"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100100"],"award-info":[{"award-number":["82100100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key R&D Program of Hunan Province","award":["2022SK2038"],"award-info":[{"award-number":["2022SK2038"]}]},{"name":"National Key Research and Development Program of China","award":["2022YFC2504401"],"award-info":[{"award-number":["2022YFC2504401"]}]},{"name":"Hunan Natural Science Youth Foundation","award":["2022JJ40810"],"award-info":[{"award-number":["2022JJ40810"]}]},{"DOI":"10.13039\/501100010031","name":"Postdoctoral Research Foundation of China","doi-asserted-by":"publisher","award":["2022M723558"],"award-info":[{"award-number":["2022M723558"]}],"id":[{"id":"10.13039\/501100010031","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of ChangSha","award":["kq2208368"],"award-info":[{"award-number":["kq2208368"]}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hunan Province of China","doi-asserted-by":"crossref","award":["2023JJ30930"],"award-info":[{"award-number":["2023JJ30930"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Major Research Project for High-Level Talents of Healthcare in Hunan Province","award":["R2023032"],"award-info":[{"award-number":["R2023032"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Next-generation sequencing (NGS) is a promising diagnostic tool for pathogens diagnosis. The aim of this study is to evaluate the application of NGS-based antimicrobial therapy on clinical outcomes in severe community-acquired pneumonia (SCAP) patients with empiric antimicrobial therapy failure.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology<\/jats:title>\n<jats:p>We performed a multi-center, retrospective cohort of SCAP patients with initial empiric therapy failure. Propensity score (PS) matching was used to obtain balance among the baseline variables in NGS group (n = 82) and conventional group (n = 82). We compared the diagnostic performance of NGS with conventional microbial culture. We also compared the impact of NGS-based antimicrobial therapy on the prognosis of patients with empirical antimicrobial therapy failure.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>The positive rate of NGS was higher than that of conventional pathogen detection methods (92.6% vs. 74.7%, <jats:italic>P<\/jats:italic> = 0.001). Compared to the conventional group, the NGS group has a considerably higher modifications rate of antibiotic treatment (73.2% vs. 54.9%, P = 0.015). The mortality of NGS group was significantly lower than that of conventional group (28.0% vs. 43.9%, <jats:italic>P<\/jats:italic> = 0.034). Moreover, the detection of NGS can significantly shorten the ventilation time (<jats:italic>P<\/jats:italic> = 0.046), and reduce the antibiotic cost (<jats:italic>P<\/jats:italic> = 0.026).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>NGS is a valuable tool for microbial identification of SCAP patient with initial empiric therapy failure.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012701","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T19:05:24Z","timestamp":1733166324000},"page":"e0012701","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Next-generation sequencing guides the treatment of severe community-acquired pneumonia with empiric antimicrobial therapy failure: A propensity-score-matched study"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3999-8253","authenticated-orcid":true,"given":"Yuhao","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7268-3167","authenticated-orcid":true,"given":"Chao","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinhua","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,2]]},"reference":[{"key":"pntd.0012701.ref001","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s00134-019-05519-y","article-title":"Challenges in severe community-acquired pneumonia: a point-of-view review","volume":"45","author":"A Torres","year":"2019","journal-title":"Intensive Care Med"},{"key":"pntd.0012701.ref002","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/S0140-6736(21)00630-9","article-title":"Community-acquired pneumonia","volume":"398","author":"S Aliberti","year":"2021","journal-title":"Lancet"},{"key":"pntd.0012701.ref003","doi-asserted-by":"crossref","first-page":"107663","DOI":"10.1016\/j.pharmthera.2020.107663","article-title":"Updates on community acquired pneumonia management in the ICU","volume":"217","author":"GB Nair","year":"2021","journal-title":"Pharmacol Ther"},{"key":"pntd.0012701.ref004","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/s00134-020-05943-5","article-title":"Critical care management of adults with community-acquired severe respiratory viral infection","volume":"46","author":"YM Arabi","year":"2020","journal-title":"Intensive Care Med"},{"key":"pntd.0012701.ref005","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s00134-019-05906-5","article-title":"Diagnosis of severe respiratory infections in immunocompromised patients","volume":"46","author":"E Azoulay","year":"2020","journal-title":"Intensive Care Med"},{"key":"pntd.0012701.ref006","doi-asserted-by":"crossref","first-page":"100906","DOI":"10.1016\/j.blre.2021.100906","article-title":"Diagnosis of infectious diseases in immunocompromised hosts using metagenomic next generation sequencing-based diagnostics","volume":"53","author":"AM Casto","year":"2022","journal-title":"Blood Rev"},{"key":"pntd.0012701.ref007","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1164\/rccm.201908-1581ST","article-title":"Diagnosis and Treatment of Adults with Community-acquired Pneumonia. 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