{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:58:36Z","timestamp":1790308716702,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012733","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p><jats:italic>Trypanosoma cruzi<\/jats:italic> is mainly transmitted to mammals by vectors, but other transmission routes exist. For example, opossums can harbor the infectious form of the parasite in their anal glands, underscoring their potential role in non-vectorial transmission. <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> has been detected in the anal gland secretions of various opossum species, and their infectivity has been confirmed in <jats:italic>Didelphis marsupialis<\/jats:italic> and <jats:italic>D<\/jats:italic>. <jats:italic>albiventris<\/jats:italic>. Vertical transmission has also been proposed in <jats:italic>D<\/jats:italic>. <jats:italic>virginiana<\/jats:italic>. However, if this occurs in opossums, it remains unclear whether it happens during pregnancy or lactation. In Mexico, <jats:italic>Didelphis virginiana<\/jats:italic> and <jats:italic>D. marsupialis<\/jats:italic> are the main opossum species. Our objective was to investigate the possible contribution of urban opossums to non-vectorial transmission of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> in the metropolitan area of Merida, Yucatan, in southeastern Mexico.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/principal findings<\/jats:title>\n<jats:p>Blood, anal gland secretions, and milk were collected from opossums captured in Merida, Mexico, all identified as <jats:italic>D<\/jats:italic>. <jats:italic>virginiana<\/jats:italic> using taxonomic keys and Cytb sequencing. By PCR, <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> was detected in 16\/102 opossums (15.69%) in at least one type of sample. The prevalence was 14.71% (15\/102) in blood and 0.98% (1\/102) in anal gland secretions. 1\/22 milk samples (4.55%) tested positive. Blood of 37 offspring from <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic>-positive mothers was collected and tested negative. qPCR revealed that females with offspring tended to have lower parasite load in blood compared to females without offspring and males. Genotyping of the parasite through multiplex PCR revealed only the DTU TcI.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/significance<\/jats:title>\n<jats:p>This study agrees with previous works where <jats:italic>D<\/jats:italic>. <jats:italic>virginiana<\/jats:italic> was the most abundant opossum species in urban areas in southeastern Mexico and confirms that it is associated with TcI. Detection of <jats:italic>T<\/jats:italic>. <jats:italic>cruzi<\/jats:italic> in a sample of anal gland secretions underscores the potential risk represented by <jats:italic>D<\/jats:italic>. <jats:italic>virginiana<\/jats:italic> in non-vectorial transmission in urban areas of southeastern Mexico. Detection in the milk of a lactating female, along with the observed tendency towards a lower parasite load in females with offspring, highlight the importance of further investigating vertical transmission in <jats:italic>D<\/jats:italic>. <jats:italic>virginiana<\/jats:italic>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012733","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T18:39:11Z","timestamp":1734115151000},"page":"e0012733","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Presence of Trypanosoma cruzi (TcI) in different tissues of Didelphis virginiana from the metropolitan area of Merida, southeastern Mexico: Epidemiological relevance and implications for non-vector transmission routes"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2738-2278","authenticated-orcid":true,"given":"Pedro Pablo","family":"Mart\u00ednez-Vega","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian","family":"Rivera-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Pellegrin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Amblard-Rambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Andr\u00e9s","family":"Calder\u00f3n-Quintal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Barnab\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Teh-Poot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Ruiz-Pi\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Ortega-Pacheco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3270-6476","authenticated-orcid":true,"given":"Etienne","family":"Waleckx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"pntd.0012733.ref001","unstructured":"WHO. Chagas disease (American trypanosomiasis), Fact sheet N\u00b0340 2023 [updated 6 de abril de 2023; cited 2023 11 de junio]. Available from: http:\/\/www.who.int\/mediacentre\/factsheets\/fs340\/en\/."},{"key":"pntd.0012733.ref002","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1146\/annurev.mi.27.100173.002023","article-title":"Biology of Trypanosoma cruzi","volume":"27","author":"Z. Brener","year":"1973","journal-title":"Annu Rev Microbiol"},{"issue":"7","key":"pntd.0012733.ref003","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1590\/S0074-02762009000700021","article-title":"A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI","volume":"104","author":"B Zingales","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"pntd.0012733.ref004","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1017\/S0031182009005861","article-title":"A new genotype of Trypanosoma cruzi associated with bats evidenced by phylogenetic analyses using SSU rDNA, cytochrome b and Histone H2B genes and genotyping based on ITS1 rDNA","volume":"136","author":"A Marcili","year":"2009","journal-title":"Parasitology"},{"issue":"8","key":"pntd.0012733.ref005","doi-asserted-by":"crossref","first-page":"e0004792","DOI":"10.1371\/journal.pntd.0004792","article-title":"Over Six Thousand Trypanosoma cruzi Strains Classified into Discrete Typing Units (DTUs): Attempt at an Inventory.","volume":"10","author":"SF Breniere","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0012733.ref006","first-page":"104","article-title":"Epidemiology, control and surveillance of Chagas disease: 100 years after its discovery","volume":"1","author":"JR Coura","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"2","key":"pntd.0012733.ref007","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.idc.2012.03.002","article-title":"American trypanosomiasis (Chagas disease).","volume":"26","author":"A Rassi","year":"2012","journal-title":"Infect Dis Clin North Am"},{"issue":"4","key":"pntd.0012733.ref008","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1590\/S0074-02761984000400021","article-title":"Trypanosoma cruzi: vertebrate and invertebrate cycles in the same mammal host, the opossum Didelphis marsupialis","volume":"79","author":"MP Deane","year":"1984","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"pntd.0012733.ref009","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1006\/expr.1997.4144","article-title":"Trypanosoma cruzi in the opossum Didelphis marsupialis: a study of the correlations and kinetics of the systemic and scent gland infections in naturally and experimentally infected animals","volume":"86","author":"AM Jansen","year":"1997","journal-title":"Exp Parasitol"},{"issue":"1","key":"pntd.0012733.ref010","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10393-013-0819-5","article-title":"Trypanosoma cruzi infection in Didelphis virginiana in relation to population parameters and variables associated with presence in rural community dwellings in Yucatan, Mexico.","volume":"10","author":"J Parada-Lopez","year":"2013","journal-title":"Ecohealth"},{"issue":"5","key":"pntd.0012733.ref011","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1590\/S0074-02762002000500003","article-title":"The opossum Didelphis virginiana as a synanthropic reservoir of Trypanosoma cruzi in Dzidzilche, Yucatan, Mexico","volume":"97","author":"HA Ruiz-Pi\u00f1a","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"pntd.0012733.ref012","doi-asserted-by":"crossref","first-page":"35","DOI":"10.4067\/S0719-81322017000100035","article-title":"Presence of Virginia opossum (Didelphis virginiana) and Pic (Triatoma dimidiata) infected with Trypanosoma cruzi in urban areas: preliminary evaluation in the city of Campeche, Mexico.","volume":"49","author":"P Tamay-Segovia","year":"2017","journal-title":"Austral journal of veterinary sciences"},{"issue":"3","key":"pntd.0012733.ref013","first-page":"216","article-title":"Prevalence of Trypanosoma cruzi infection in dogs and small mammals in Nuevo Leon, Mexico.","volume":"49","author":"L Galaviz-Silva","year":"2017","journal-title":"Rev Argent Microbiol"},{"issue":"4","key":"pntd.0012733.ref014","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1590\/S0036-46651996000400009","article-title":"Infection by Trypanosoma cruzi in mammals in Yucatan, Mexico: a serological and parasitological study.","volume":"38","author":"J Zavala-Velazquez","year":"1996","journal-title":"Revista do Instituto de Medicina Tropical de Sao Paulo"},{"issue":"1","key":"pntd.0012733.ref015","first-page":"51","article-title":"[Mechanisms of transmission of the etiological agent of Chagas\u2019 disease].","volume":"21","author":"RD Ribeiro","year":"1987","journal-title":"Rev Saude Publica"},{"issue":"6","key":"pntd.0012733.ref016","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1590\/S0074-02761995000600003","article-title":"Interaction between Didelphis albiventris and Triatoma infestans in relation to Trypanosoma cruzi transmission","volume":"90","author":"NJ Schweigmann","year":"1995","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"6","key":"pntd.0012733.ref017","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.2307\/3278009","article-title":"Transmission of Trypanosoma cruzi infection to opossums via the oral route","volume":"57","author":"RG Yaeger","year":"1971","journal-title":"J Parasitol"},{"issue":"3","key":"pntd.0012733.ref018","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1006\/expr.2001.4603","article-title":"Trypanosoma cruzi in the scent glands of Didelphis marsupialis: the kinetics of colonization","volume":"97","author":"JC Carreira","year":"2001","journal-title":"Exp Parasitol"},{"key":"pntd.0012733.ref019","first-page":"3","volume-title":"Wild Mammals of North America.","author":"A Gardner","year":"2003"},{"issue":"4","key":"pntd.0012733.ref020","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1590\/S0074-02761996000400002","article-title":"Trypanosoma cruzi in the anal glands of urban opossums. I\u2014Isolation and experimental infections","volume":"91","author":"S Urdaneta-Morales","year":"1996","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"pntd.0012733.ref021","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1590\/S0074-02761989000100016","article-title":"Infec\u00e7\u00e3o natural das gl\u00e2ndulas anais de gamb\u00e1s (Didelphis albiventris) pelo Trypanosoma cruzi no munic\u00edpio de Bambu\u00ed - MG.","volume":"84","author":"AJ Fernandes","year":"1989","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"issue":"3","key":"pntd.0012733.ref022","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0014-4894(91)90096-F","article-title":"Trypanosoma cruzi in the opossum Didelphis marsupialis: parasitological and serological follow-up of the acute infection","volume":"73","author":"AM Jansen","year":"1991","journal-title":"Exp Parasitol"},{"key":"pntd.0012733.ref023","first-page":"100463","article-title":"First report of natural infection by Trypanosoma cruzi in secretions of the scent glands and myocardium of Philander opossum (Marsupialia: Didelphidae): Parasitological and clinicopathological findings.","volume":"22","author":"FNL Barros","year":"2020","journal-title":"Vet Parasitol Reg Stud Reports"},{"key":"pntd.0012733.ref024","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.ijppaw.2020.03.004","article-title":"Trypanosoma cruzi infections and associated pathology in urban-dwelling Virginia opossums (Didelphis virginiana).","volume":"11","author":"IB Zecca","year":"2020","journal-title":"Int J Parasitol Parasites Wildl"},{"issue":"6","key":"pntd.0012733.ref025","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1590\/S0074-02761995000600005","article-title":"Search for Trypanosoma cruzi in the anal glands of wild Didelphis albiventris from Santiago del Estero, Argentina","volume":"90","author":"O Conti","year":"1995","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"pntd.0012733.ref026","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1590\/S0074-02761988000100017","article-title":"Presence of Trypanosoma cruzi in the anal glands of naturally infected opossum (Didelphis marsupialis) in the state of Santa Catarina, Brazil.","volume":"83","author":"M Steindel","year":"1988","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"12","key":"pntd.0012733.ref027","doi-asserted-by":"crossref","first-page":"e0010974","DOI":"10.1371\/journal.pntd.0010974","article-title":"Identification of the parasite, Trypanosoma cruzi, in multiple tissues of epidemiological significance in the Virginia opossum (Didelphis virginiana): Implications for environmental and vertical transmission routes.","volume":"16","author":"CW Torhorst","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0012733.ref028","volume-title":"Metr\u00f3polis de M\u00e9xico 2020. Primera ed: Secretar\u00eda de Desarrollo Agrario","author":"SEDATU, CONAPO, INEGI","year":"2024"},{"issue":"4","key":"pntd.0012733.ref029","doi-asserted-by":"crossref","first-page":"364","DOI":"10.2307\/1375212","article-title":"Sex and Age Determination in the Opossum","volume":"30","author":"GA Petrides","year":"1949","journal-title":"Journal of Mammalogy"},{"issue":"7","key":"pntd.0012733.ref030","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"S Kumar","year":"2016","journal-title":"Mol Biol Evol"},{"issue":"22","key":"pntd.0012733.ref031","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"JD Thompson","year":"1994","journal-title":"Nucleic Acids Res"},{"key":"pntd.0012733.ref032","article-title":"editor BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","author":"TA Hall","year":"1999","journal-title":"Nucleic acids symposium series"},{"issue":"12","key":"pntd.0012733.ref033","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1093\/molbev\/msx248","article-title":"DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets","volume":"34","author":"J Rozas","year":"2017","journal-title":"Mol Biol Evol"},{"issue":"7","key":"pntd.0012733.ref034","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1128\/jcm.27.7.1477-1482.1989","article-title":"Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction","volume":"27","author":"DR Moser","year":"1989","journal-title":"J Clin Microbiol"},{"issue":"1","key":"pntd.0012733.ref035","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0166-6851(03)00093-8","article-title":"Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR","volume":"129","author":"KL Cummings","year":"2003","journal-title":"Mol Biochem Parasitol"},{"issue":"5","key":"pntd.0012733.ref036","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.meegid.2010.11.013","article-title":"Further interest of miniexon multiplex PCR for a rapid typing of Trypanosoma cruzi DTU groups","volume":"11","author":"C Aliaga","year":"2011","journal-title":"Infect Genet Evol"},{"issue":"1","key":"pntd.0012733.ref037","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0035-9203(01)90350-5","article-title":"A mini-exon multiplex polymerase chain reaction to distinguish the major groups of Trypanosoma cruzi and T. rangeli in the Brazilian Amazon","volume":"95","author":"O Fernandes","year":"2001","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"pntd.0012733.ref038","volume":"1633","author":"LD Brown","year":"2001","journal-title":"Interval Estimation for a Binomial Proportion"},{"issue":"4","key":"pntd.0012733.ref039","doi-asserted-by":"crossref","first-page":"e0006859","DOI":"10.1371\/journal.pntd.0006859","article-title":"Estimating the current burden of Chagas disease in Mexico: A systematic review and meta-analysis of epidemiological surveys from 2006 to 2017.","volume":"13","author":"A Arnal","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"pntd.0012733.ref040","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.actatropica.2013.04.007","article-title":"Chagas disease (American trypanosomiasis) in Mexico: an update.","volume":"127","author":"A Carabarin-Lima","year":"2013","journal-title":"Acta Trop"},{"issue":"4","key":"pntd.0012733.ref041","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1590\/S0074-02762006000400001","article-title":"Chagas disease in Mexico: an analysis of geographical distribution during the past 76 years\u2014a review","volume":"101","author":"A Cruz-Reyes","year":"2006","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"pntd.0012733.ref042","doi-asserted-by":"crossref","first-page":"107038","DOI":"10.1016\/j.actatropica.2023.107038","article-title":"Temporal variation of Triatoma dimidiata abundance and infection with Trypanosoma cruzi in domestic and sylvatic habitats of rural Yucatan","volume":"248","author":"JI Moo-Millan","year":"2023","journal-title":"Mexico. Acta Trop"},{"issue":"2","key":"pntd.0012733.ref043","doi-asserted-by":"crossref","first-page":"176","DOI":"10.4269\/ajtmh.2002.67.176","article-title":"Geographic distribution of Triatoma dimidiata and transmission dynamics of Trypanosoma cruzi in the Yucatan peninsula of Mexico","volume":"67","author":"E Dumonteil","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0012733.ref044","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1590\/S0074-02762000000600008","article-title":"Trypanosoma cruzi infection in Didelphis marsupialis in Santa Catarina and Arvoredo Islands, southern Brazil.","volume":"95","author":"EC Grisard","year":"2000","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz."},{"issue":"1","key":"pntd.0012733.ref045","doi-asserted-by":"crossref","first-page":"36","DOI":"10.4269\/ajtmh.23-0380","article-title":"An Outbreak of Acute Chagas Disease Possibly Spread through Oral Transmission Involving Animal Reservoirs in Eastern Colombia","volume":"110","author":"SA Gutierrez","year":"2024","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0012733.ref046","first-page":"2","author":"G Dobigny","year":"2022","journal-title":"Zoonotic emergence at the animal-environment-human interface: the forgotten urban socio-ecosystems %J Peer Community Journal"},{"key":"pntd.0012733.ref047","doi-asserted-by":"crossref","first-page":"100005","DOI":"10.24072\/pci.infections.100005","article-title":"Zoonotic emergence and the overlooked case of cities.","author":"E. Waleckx","year":"2022","journal-title":"Peer Community in Infections."},{"issue":"4","key":"pntd.0012733.ref048","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1089\/vbz.2007.0133","article-title":"Urban infestation by Triatoma dimidiata in the city of Merida, Yucatan, Mexico.","volume":"7","author":"Y Guzman-Tapia","year":"2007","journal-title":"Vector Borne Zoonotic Dis"},{"key":"pntd.0012733.ref049","first-page":"101059","article-title":"Circulation of Trypanosoma cruzi in triatomines and Didelphis sp. in urban areas: Transmission risk assessment in the Metropolitan Region. Veterinary Parasitology","volume":"52","author":"RA Silva","year":"2024","journal-title":"Regional Studies and Reports"},{"issue":"1","key":"pntd.0012733.ref050","doi-asserted-by":"crossref","first-page":"4140","DOI":"10.1038\/s41598-018-22455-x","article-title":"Detailed ecological associations of triatomines revealed by metabarcoding and next-generation sequencing: implications for triatomine behavior and Trypanosoma cruzi transmission cycles.","volume":"8","author":"E Dumonteil","year":"2018","journal-title":"Sci Rep."},{"issue":"1","key":"pntd.0012733.ref051","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1186\/s13071-019-3819-7","article-title":"Disentangling Trypanosoma cruzi transmission cycle dynamics through the identification of blood meal sources of natural populations of Triatoma dimidiata in Yucatan, Mexico.","volume":"12","author":"JI Moo-Millan","year":"2019","journal-title":"Parasit Vectors."},{"key":"pntd.0012733.ref052","first-page":"125","article-title":"H\u00e1bitos biol\u00f3gicos de Triatoma dimidiata en el estado de Yucat\u00e1n, M\u00e9xico.","volume":"3","author":"ES Guzman-Marin","year":"1992","journal-title":"Rev Biomed"},{"key":"pntd.0012733.ref053","first-page":"62","article-title":"Enfermedad de Chagas, del sue\u00f1o, y leishmaniasis: \u00bfCu\u00e1les son los efectos de la antropizaci\u00f3n sobre la transmisi\u00f3n de los par\u00e1sitos tripanosom\u00e1tidos?","volume":"2","author":"A Hern\u00e1ndez Andrade","year":"2022","journal-title":"Boletin de la Sociedad Cientifica Mexicana de Ecologia"},{"issue":"1","key":"pntd.0012733.ref054","doi-asserted-by":"crossref","first-page":"176","DOI":"10.4269\/ajtmh.1977.26.176","article-title":"Feeding preferences of Triatoma dimidiata maculipennis in Yucatan, Mexico","volume":"26","author":"RE Quintal","year":"1977","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pntd.0012733.ref055","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s00436-012-3184-3","article-title":"Trypanosoma cruzi experimental congenital transmission associated with TcV and TcI subpatent maternal parasitemia","volume":"112","author":"SM Alkmim-Oliveira","year":"2013","journal-title":"Parasitol Res"},{"issue":"3","key":"pntd.0012733.ref056","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.ajpath.2022.11.010","article-title":"Effects of Acute and Chronic Trypanosoma cruzi Infection on Pregnancy Outcomes in Mice: Parasite Transmission, Mortality, Delayed Growth, and Organ Damage in Pups","volume":"193","author":"LE Rios","year":"2023","journal-title":"Am J Pathol"},{"issue":"9","key":"pntd.0012733.ref057","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1089\/vbz.2022.0086","article-title":"Congenital Transmission of Trypanosoma cruzi in Naturally Infected Dogs.","volume":"23","author":"EE Avalos-Borges","year":"2023","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"2","key":"pntd.0012733.ref058","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0002-9440(10)64223-X","article-title":"Acute Trypanosoma cruzi infection in mouse induces infertility or placental parasite invasion and ischemic necrosis associated with massive fetal loss","volume":"161","author":"A Mjihdi","year":"2002","journal-title":"Am J Pathol"},{"issue":"2","key":"pntd.0012733.ref059","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1645\/GE-1740.1","article-title":"Oral transmission of Trypanosoma cruzi with opposing evidence for the theory of carnivory","volume":"95","author":"DM Roellig","year":"2009","journal-title":"J Parasitol"},{"issue":"2","key":"pntd.0012733.ref060","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ijpara.2004.10.024","article-title":"Origins of Chagas disease: Didelphis species are natural hosts of Trypanosoma cruzi I and armadillos hosts of Trypanosoma cruzi II, including hybrids","volume":"35","author":"M Yeo","year":"2005","journal-title":"Int J Parasitol"},{"key":"pntd.0012733.ref061","doi-asserted-by":"crossref","first-page":"114","DOI":"10.21149\/13801","article-title":"Trypanosoma cruzi in Mexican Neotropical vectors and mammals: wildlife, livestock, pets, and human population.","volume":"65","author":"A Izeta-Alberdi","year":"2023","journal-title":"Salud Publica Mex."},{"key":"pntd.0012733.ref062","article-title":"Trypanosoma cruzi in Wild and Synanthropic Mammals in Two Regions of Mexico: A Fieldwork and Genetic Discrete Typing Unit Review.","author":"JA Martinez Ibarra","year":"2024","journal-title":"Vector Borne Zoonotic Dis."}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0012733","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012733","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T18:40:57Z","timestamp":1735324857000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012733"}},"subtitle":[],"editor":[{"given":"Laura-Isobel","family":"McCall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,13]]},"references-count":62,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0012733","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0012733","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,13]]}}}