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While the host-pathogen interactions of <jats:italic>T. cruzi<\/jats:italic> have been extensively studied in vertebrate models, investigations into its interactions within its insect host remain limited. To address this gap and establish a genetically tractable system for studying parasite-vector dynamics, we conducted quantitative kinetic infection studies using the Y strain of <jats:italic>T. cruzi<\/jats:italic> and the model vector <jats:italic>Rhodnius prolixus<\/jats:italic>. We began by comparing parasite infection kinetics from two genetically diverse strains of <jats:italic>T. cruzi,<\/jats:italic> Brazil and Y, and demonstrated that ingested parasites from both strains transiently expand in the anterior regions of the insect digestive tract with stable colonization occurring in the hindgut over the long term. Notably, we demonstrated that the clonal Y strain, contrary to previous reports, can effectively infect and persist across multiple developmental stages of <jats:italic>R. prolixus<\/jats:italic>. Additionally, comparison of movement of parasites versus inert fluorescent microspheres introduced into artificial blood meals suggests that <jats:italic>T. cruzi<\/jats:italic> colonization of the <jats:italic>R. prolixus<\/jats:italic> gut occurs passively through peristaltic movement during digestion, rather than through active parasite-mediated chemotaxis. These findings highlight the <jats:italic>T. cruzi<\/jats:italic> Y strain - <jats:italic>R. prolixus<\/jats:italic> model system as a promising tool for the in-depth molecular characterization of parasite-vector interactions, potentially offering new insights into the biology of this neglected and deadly human pathogen.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012906","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T17:33:01Z","timestamp":1741800781000},"page":"e0012906","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stable colonization of the kissing bug Rhodnius prolixus by Trypanosoma cruzi Y strain"],"prefix":"10.1371","volume":"19","author":[{"given":"Ruby E.","family":"Harrison","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin J.","family":"Vogel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9144-287X","authenticated-orcid":true,"given":"Ronald Drew","family":"Etheridge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"issue":"4","key":"pntd.0012906.ref001","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1128\/CMR.00005-11","article-title":"Trypanosoma cruzi and Chagas\u2019 Disease in the United States","volume":"24","author":"C Bern","year":"2011","journal-title":"Clin Microbiol Rev"},{"issue":"3","key":"pntd.0012906.ref002","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.gheart.2015.06.001","article-title":"The burden of Chagas disease: estimates and challenges","volume":"10","author":"JD Stanaway","year":"2015","journal-title":"Glob Heart"},{"issue":"9","key":"pntd.0012906.ref003","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/clc.22421","article-title":"The Epidemiology, clinical manifestations, and management of chagas heart disease","volume":"38","author":"LH Malik","year":"2015","journal-title":"Clin Cardiol"},{"issue":"3","key":"pntd.0012906.ref004","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s40506-018-0170-z","article-title":"Treatment of Chagas Disease in the United States","volume":"10","author":"S Meymandi","year":"2018","journal-title":"Curr Treat Options Infect Dis"},{"issue":"5","key":"pntd.0012906.ref005","doi-asserted-by":"crossref","first-page":"165658","DOI":"10.1016\/j.bbadis.2019.165658","article-title":"Chagas disease vaccine design: the search for an efficient Trypanosoma cruzi immune-mediated control","author":"AE Bivona","year":"2020","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"pntd.0012906.ref006","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1051\/parasite\/2014012","article-title":"Chagas disease in the 21st century: a public health success or an emerging threat?","volume":"21","author":"KM Bonney","year":"2014","journal-title":"Parasite"},{"issue":"3","key":"pntd.0012906.ref007","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1590\/0074-0276140362","article-title":"The main sceneries of Chagas disease transmission. 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