{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T17:44:21Z","timestamp":1761241461644,"version":"build-2065373602"},"reference-count":93,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010869","name":"Stanford Woods Institute for the Environment","doi-asserted-by":"publisher","award":["281131"],"award-info":[{"award-number":["281131"]}],"id":[{"id":"10.13039\/100010869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","award":["2020281023"],"award-info":[{"award-number":["2020281023"]}],"id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009924","name":"Air Force Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019658","name":"John A. Blume Earthquake Engineering Center, Stanford University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019658","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["None"],"award-info":[{"award-number":["None"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Soil-transmitted helminths, like\n                    <jats:italic>\n                      <jats:italic>Ascaris lumbricoides,<\/jats:italic>\n                    <\/jats:italic>\n                    are significant contributors to disease burden in low- and middle-income countries (LMICs). Infections are associated with morbidity and mortality in children and are often transmitted through eggs in fecally contaminated soil. Interventions, like replacing household soil floors with cement-based alternatives, may reduce exposure to\n                    <jats:italic>\n                      <jats:italic>A. lumbricoides<\/jats:italic>\n                    <\/jats:italic>\n                    eggs, but there are currently no estimates on the removal or survival of\n                    <jats:italic>\n                      <jats:italic>Ascaris<\/jats:italic>\n                    <\/jats:italic>\n                    species eggs on cement-based surfaces. This study addresses that knowledge gap by evaluating the removal of\n                    <jats:italic>\n                      <jats:italic>Ascaris suum<\/jats:italic>\n                    <\/jats:italic>\n                    eggs from mopping and the survival of\n                    <jats:italic>\n                      <jats:italic>A. suum<\/jats:italic>\n                    <\/jats:italic>\n                    eggs on two cement-based mixes: an traditional mortar and a mortar with fly ash, which provides a more sustainable alternative to the traditional mortar mix. We assessed egg survival at two temperatures representing the dry (15\u00b0C) and wet (34\u00b0C) seasons in Bangladesh using two different egg enumeration methods. After mopping, a mean of 95.6% (SD\u2009=\u20094.0%) of viable eggs were removed from surfaces, with no significant differences between cement-based mixes (p\u2009=\u20090.51). The mean first-order decay rate constants (\n                    <jats:italic>\n                      <jats:italic>k<\/jats:italic>\n                    <\/jats:italic>\n                    ) of\n                    <jats:italic>\n                      <jats:italic>A. suum<\/jats:italic>\n                    <\/jats:italic>\n                    eggs across all conditions was 0.029 day\n                    <jats:sup>-1<\/jats:sup>\n                    (SD\u2009=\u20090.074 day\n                    <jats:sup>-1<\/jats:sup>\n                    ). Values of\n                    <jats:italic>\n                      <jats:italic>k<\/jats:italic>\n                    <\/jats:italic>\n                    were similar between mix designs (p\u2009=\u20090.62) but varied significantly between temperatures (p\u2009=\u20094.2x10\n                    <jats:sup>-25<\/jats:sup>\n                    ) and egg enumeration methods (p\u2009=\u20092.4x10\n                    <jats:sup>-8<\/jats:sup>\n                    ). The\n                    <jats:italic>\n                      <jats:italic>k<\/jats:italic>\n                    <\/jats:italic>\n                    values were greater at 34\u00b0C compared to at 15\u00b0C, where they showed no significant inactivation. Our\n                    <jats:italic>\n                      <jats:italic>k<\/jats:italic>\n                    <\/jats:italic>\n                    values were comparable to those reported previously for different matrices, indicating comparable inactivation of\n                    <jats:italic>\n                      <jats:italic>Ascaris<\/jats:italic>\n                    <\/jats:italic>\n                    species eggs on cement-based surfaces compared to liquid and semi-solid matrices. These results provide some of the first estimates of removal efficiencies and decay rate constants in realistic environmental conditions for\n                    <jats:italic>\n                      <jats:italic>Ascaris<\/jats:italic>\n                    <\/jats:italic>\n                    species on surfaces while supporting the use of mortar mix designs with fly ash in interventions to reduce\n                    <jats:italic>\n                      <jats:italic>Ascaris<\/jats:italic>\n                    <\/jats:italic>\n                    species transmission in rural LMIC households.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0012919","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T17:40:08Z","timestamp":1761241208000},"page":"e0012919","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating cement-based surfaces as a sustainable flooring solution to improve Ascaris Suum egg removal and inactivation in low-resource settings"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9626-4964","authenticated-orcid":true,"given":"Claire E.","family":"Anderson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhi","family":"Hanif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshika","family":"Crider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Lepech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Billington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandria B.","family":"Boehm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jade","family":"Benjamin-Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"pntd.0012919.ref001","unstructured":"World Health Organization. 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