{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T22:13:46Z","timestamp":1781648026801,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013008","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000}}],"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","award":["Not applicable"],"award-info":[{"award-number":["Not applicable"]}],"id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Cryptococcal meningitis is one of the top causes of morbidity and mortality in people living with HIV\/AIDS. In high prevalence regions, current recommendations are to screen individuals with blood CD4<jats:sup>+<\/jats:sup> T cell counts less than 200 cells\/\u00b5l for serum cryptococcal antigen (CrAg) and then preemptively treat those who test positive for presumed cryptococcosis. However, in many low-resource settings, including Monrovia, Liberia, flow cytometric CD4 assays are not readily available. We tested subjects with known HIV infection using a lateral flow assay (LFA), which provides a semi-quantitative determination of whether the blood CD4<jats:sup>+<\/jats:sup> T cell count is \u2264200 cells\/\u00b5l. Subjects with counts \u2264200 cells\/\u00b5l were then tested with an LFA that detects CrAg. Of the 500 HIV+ subjects tested, 201 (40.2%) had blood CD4<jats:sup>+<\/jats:sup> T cell count \u2264200. Of those, 82\/201 (40.7%) were serum CrAg+. Subjects who were serum CrAg+ were more likely to have a Glasgow Coma Score &lt;15, whereas subjects who were CrAg- were more likely to be HIV-2+. Lumbar punctures were performed on 61 serum CrAg+ subjects; 30\/61 (49.2%) subjects were cerebrospinal fluid CrAg+. Thus, sequential point-of-care testing enabled the diagnosis of cryptococcosis in HIV+ individuals with blood CD4 T cell counts \u2264200 cells\/\u00b5l. As diagnostic testing informs life-saving therapies, it is imperative that these assays are made readily available in resource-poor settings.<\/jats:p>","DOI":"10.1371\/journal.pntd.0013008","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T18:24:07Z","timestamp":1744136647000},"page":"e0013008","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Use of sequential lateral flow assays to diagnose cryptococcal infection among people living with HIV in Monrovia, Liberia"],"prefix":"10.1371","volume":"19","author":[{"given":"Yassah M.","family":"Barclay-Korboi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Adeel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Ajami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flinhway Hessou","family":"Dickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Wachekwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nyenyakar A. 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