{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T11:06:38Z","timestamp":1789556798109,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013016","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000}}],"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Actinomadura<\/jats:italic> isolates obtained from seven human mycetoma cases in Mexico were characterized using nucleotide sequence analysis of a portion of the small subunit ribosomal RNA gene. Most isolates were identified as <jats:italic>Actinomadura madurae.<\/jats:italic> However, one isolate, LIID-AQ337, showed inconclusive results. To determine its identity, genomic DNA from LIID-AQ337 was subjected to whole-genome sequencing using both short- and long-read sequencing, resulting in a genome of 8,163,638-bp spread in 22 contigs. Comparative analysis against currently available genomes of other <jats:italic>Actinomadura<\/jats:italic> species suggested that LIID-AQ337 belongs to a new <jats:italic>Actinomadura<\/jats:italic> species. 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