{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:15:15Z","timestamp":1787955315224,"version":"build-2784847793"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013048","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000}}],"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020953","name":"Taiwan Centers for Disease Control","doi-asserted-by":"publisher","award":["MOHW114-CDC-C-315-124112"],"award-info":[{"award-number":["MOHW114-CDC-C-315-124112"]}],"id":[{"id":"10.13039\/100020953","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"\nBackground<\/jats:title>\nSalmonella enterica<\/jats:italic><\/jats:italic> serovar Paratyphi A (S.<\/jats:italic><\/jats:italic> Paratyphi A) has emerged as a significant global health concern due to the progressive development of antimicrobial resistance and its broader geographic distribution. In Taiwan, paratyphoid fever was historically rare and predominantly associated with imported cases. Since 2022, however, a marked increase in domestically acquired infections has been observed, prompting investigations into their origin and likely route of introduction.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nWe analyzed surveillance data on 223 patients with paratyphoid fever reported in Taiwan between January 2001 and December 2024. Whole-genome sequencing and antimicrobial susceptibility testing were performed on 88 S.<\/jats:italic><\/jats:italic> Paratyphi A isolates obtained from both imported and domestically acquired infections from 2007 to 2024. Phylogenetic analysis and genotyping were conducted to assess genetic relatedness and to trace potential sources of introduction by comparing them with global isolates.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nAlthough 55.2% of paratyphoid fever infections were imported, domestically acquired infections became predominant after 2022. Most isolates (76.1%) were resistant to nalidixic acid and nonsusceptible to ciprofloxacin due to gyrA<\/jats:italic><\/jats:italic> mutations at codon 83 (S83F or S83Y). The majority of domestic isolates were classified as ST129 and paratype 2.4 and showed close genetic relatedness to strains from Indonesia. Of the 31 domestic isolates collected between 2022 and 2024, 30 clustered with Indonesian strains, and 28 exhibited nearly identical genomic profiles, which suggested a prolonged outbreak likely linked to a common external source, such as contaminated imported food.<\/jats:p>\n<\/jats:sec>\n\nConclusions<\/jats:title>\nThe genomic evidence suggests that the recent increase in domestically acquired S.<\/jats:italic><\/jats:italic> Paratyphi A infections in Taiwan represents a prolonged outbreak rather than a sustained epidemiological shift. These infections were closely related to strains from Indonesia, suggesting a potential epidemiological link between the two countries in the transmission of paratyphoid fever. While 76.1% of isolates were nonsusceptible to ciprofloxacin due to gyrA<\/jats:italic><\/jats:italic> mutations, susceptibility to traditional first-line agents remained high. The observed decline in case numbers in 2024 may indicate that the outbreak is subsiding. Genomic surveillance played a crucial role in tracing sources of infection and informing targeted public health responses.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0013048","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T17:45:06Z","timestamp":1757612706000},"page":"e0013048","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Paratyphoid fever and the genomics of Salmonella enterica serovar Paratyphi A in 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