{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:50:17Z","timestamp":1789753817624,"version":"4.0.1"},"reference-count":67,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Hebei Natural Science Foundation","doi-asserted-by":"crossref","award":["C2023205011"],"award-info":[{"award-number":["C2023205011"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foundation of Hebei Normal University","award":["L2023B56"],"award-info":[{"award-number":["L2023B56"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31472050"],"award-info":[{"award-number":["31472050"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Anti-tick vaccines are gaining attention as a strategy to prevent tick infestations by activating the immune response of the host. Antibodies produced by the host inhibit tick growth and reproduction, but the molecular mechanism remains to be clarified. In this study, we investigated the effects of cystatin antibodies on the ovarian function of <jats:italic>Haemaphysalis doenitzi<\/jats:italic>. Histological analysis revealed that exposure to cystatin antibodies resulted in a significant reduction in the number of eggs produced and caused severe damage to the ovarian tissue structure. Immunofluorescence experiments confirmed the significant expression of cystatin within the ovary. Proteomics and phosphoproteomics identified 31 and 10 differentially expressed proteins in the relevant pathways, respectively. These changes in protein levels were found to be regulated by various mechanisms, including ribosomes activity, regulation of actin cytoskeleton, RNA transport, the TCA cycle, drug metabolism, and mTOR signaling pathways. Notably, there was high expression of tropomyosin and low expression of glutathione S-transferase (GST) during ovarian detoxification. Enzyme activity assays indicated a significant down-regulation of GST enzyme activity in the immunized group, suggesting that cystatin antibodies impaired the detoxification capacity of the ticks. Both tropomyosin and GST were successfully cloned and designated as HD-TPMa and HD-GSTa, respectively. RNA interference (RNAi) successfully knocked down the target gene. Ticks subjected to immersion in cystatin antibodies exhibited a significantly increased mortality rate after 72 hours. This study elucidated the molecular mechanism by which cystatin antibodies inhibit the growth and development of tick ovaries, providing an important scientific basis for the development of effective tick ovary control strategies.<\/jats:p>","DOI":"10.1371\/journal.pntd.0013064","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T17:43:23Z","timestamp":1746639803000},"page":"e0013064","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cystatin antibodies interfere with ovary development in Haemaphysalis doenitzi (Acari: Ixodidae)"],"prefix":"10.1371","volume":"19","author":[{"given":"Songbo","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed H.","family":"Ghonaim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijia","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5761-0262","authenticated-orcid":true,"given":"Xiaolong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,5,7]]},"reference":[{"issue":"8","key":"pntd.0013064.ref001","doi-asserted-by":"crossref","first-page":"3957","DOI":"10.1002\/ps.8099","article-title":"The evaluation of cystatin protein vaccines based on the stress response of ticks triggered by low-temperature and toxin stress in Haemaphysalis doenitzi","volume":"80","author":"S-B Zhang","year":"2024","journal-title":"Pest Manag Sci"},{"key":"pntd.0013064.ref002","doi-asserted-by":"crossref","first-page":"106075","DOI":"10.1016\/j.pestbp.2024.106075","article-title":"Functional analysis of novel cystatins from Haemaphysalis doenitzi and evaluation of their roles in cypermethrin and \u03bb-cyhalothrin resistance","volume":"204","author":"S Zhang","year":"2024","journal-title":"Pestic Biochem Physiol"},{"issue":"12","key":"pntd.0013064.ref003","doi-asserted-by":"crossref","first-page":"e0010980","DOI":"10.1371\/journal.pntd.0010980","article-title":"Expression of Concern: Tick-borne relapsing fever Borreliosis, a major public health problem overlooked in Senegal","volume":"16","author":"PLOS Neglected Tropical Diseases Editors","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013064.ref004","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.2307\/3278650","article-title":"The Haemaphysalis ticks (Ixodoidea: Ixodidae) of birds. 3. H. (Ornithophysalis) subgen. n.: definition, species, hosts, and distribution in the Oriental, Palearctic, Malagasy, and Ethiopian faunal regions","volume":"59","author":"H Hoogstraal","year":"1973","journal-title":"The Journal of parasitology"},{"key":"pntd.0013064.ref005","first-page":"1","article-title":"Discovery of a novel Mediterranean Haemaphysalis (Ornithophysalis) doenitzi group tick species infesting Falco eleonorae on Antikythira Island, Greece","author":"L Chitimia-Dobler","year":"2024","journal-title":"Parasitology"},{"key":"pntd.0013064.ref006","doi-asserted-by":"crossref","first-page":"815","DOI":"10.46234\/ccdcw2023.156","article-title":"Molecular Identification and Genetic Characterization of Public Health Threatening Ticks - Chongming Island, China, 2021-2022","volume":"5","author":"S Fei","year":"2023","journal-title":"China CDC weekly"},{"key":"pntd.0013064.ref007","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.3201\/eid3012.240539","article-title":"Transboundary Movement of Yezo Virus via Ticks on Migratory Birds, Japan, 2020-2021","volume":"30","author":"A Nishino","year":"2024","journal-title":"Emerging infectious diseases"},{"issue":"7","key":"pntd.0013064.ref008","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1002\/jemt.23693","article-title":"Cytotoxic effects of Satureja montana L. essential oil on oocytes of engorged Rhipicephalus microplus female ticks (Acari: Ixodidae)","volume":"84","author":"AC Reis","year":"2021","journal-title":"Microsc Res Tech"},{"issue":"1\u20132","key":"pntd.0013064.ref009","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10493-023-00809-z","article-title":"Toxic effect of Callistemon viminalis essential oil on the ovary of engorged Rhipicephalus microplus female ticks (Acari: Ixodidae)","volume":"90","author":"AC Reis","year":"2023","journal-title":"Exp Appl Acarol"},{"issue":"2","key":"pntd.0013064.ref010","doi-asserted-by":"crossref","first-page":"102289","DOI":"10.1016\/j.ttbdis.2023.102289","article-title":"Tick salivary protein Cystatin: structure, anti-inflammation and molecular mechanism","volume":"15","author":"X Gao","year":"2024","journal-title":"Ticks Tick Borne Dis"},{"key":"pntd.0013064.ref011","doi-asserted-by":"crossref","first-page":"101507","DOI":"10.1016\/j.ttbdis.2020.101507","article-title":"Ultrastructural changes induced by the entomopathogenic fungus Beauveria bassiana in the ovary of the tick Argas (Persicargas) persicus (Oken","volume":"11","author":"AS Marzouk","year":"2020","journal-title":"). Ticks and tick-borne diseases"},{"issue":"1","key":"pntd.0013064.ref012","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/s13071-018-2667-1","article-title":"Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding","volume":"11","author":"EP Hernandez","year":"2018","journal-title":"Parasit Vectors"},{"issue":"6","key":"pntd.0013064.ref013","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1111\/1744-7917.12733","article-title":"Deleterious effects of electron beam irradiation on development and reproduction of tomato\/potato psyllids, Bactericera cockerelli","volume":"27","author":"J Lei","year":"2020","journal-title":"Insect Sci"},{"issue":"1","key":"pntd.0013064.ref014","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1756-3305-1-7","article-title":"Profiling of proteolytic enzymes in the gut of the tick Ixodes ricinus reveals an evolutionarily conserved network of aspartic and cysteine peptidases","volume":"1","author":"D Sojka","year":"2008","journal-title":"Parasit Vectors"},{"issue":"1","key":"pntd.0013064.ref015","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ijpara.2008.06.012","article-title":"Legumains from the hard tick Haemaphysalis longicornis play modulatory roles in blood feeding and gut cellular remodelling and impact on embryogenesis","volume":"39","author":"MA Alim","year":"2009","journal-title":"Int J Parasitol"},{"issue":"4","key":"pntd.0013064.ref016","doi-asserted-by":"crossref","first-page":"e0123841","DOI":"10.1371\/journal.pone.0123841","article-title":"Biochemical, transcriptomic and proteomic analyses of digestion in the scorpion Tityus serrulatus: insights into function and evolution of digestion in an ancient arthropod","volume":"10","author":"FJ Fuzita","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"pntd.0013064.ref017","doi-asserted-by":"crossref","first-page":"14224","DOI":"10.1038\/s41598-018-32664-z","article-title":"Tick galactosyltransferases are involved in \u03b1-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development","volume":"8","author":"A Cabezas-Cruz","year":"2018","journal-title":"Sci Rep"},{"key":"pntd.0013064.ref018","doi-asserted-by":"crossref","first-page":"775371","DOI":"10.3389\/fcimb.2021.775371","article-title":"Alpha-Gal Syndrome: Involvement of Amblyomma americanum \u03b1-D-Galactosidase and \u03b2-1,4 Galactosyltransferase Enzymes in \u03b1-Gal Metabolism","volume":"11","author":"SR Sharma","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"pntd.0013064.ref019","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/15548627.2022.2071381","article-title":"Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation","volume":"19","author":"X Cheng","year":"2023","journal-title":"Autophagy"},{"key":"pntd.0013064.ref020","doi-asserted-by":"crossref","first-page":"1236785","DOI":"10.3389\/fcimb.2023.1236785","article-title":"Integrative analysis highlights molecular and immune responses of tick Amblyomma americanum to Escherichia coli challenge","volume":"13","author":"B Lyu","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"pntd.0013064.ref021","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1083\/jcb.200110013","article-title":"Tropomyosin inhibits ADF\/cofilin-dependent actin filament dynamics","volume":"156","author":"S Ono","year":"2002","journal-title":"J Cell Biol"},{"issue":"3","key":"pntd.0013064.ref022","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1002\/ps.7856","article-title":"Comparative transcriptome and proteome reveal the unique genes and proteins of female parasitic wasps, Lysiphlebia japonica Ashmead","volume":"80","author":"J Li","year":"2024","journal-title":"Pest Manag Sci"},{"issue":"11","key":"pntd.0013064.ref023","doi-asserted-by":"crossref","first-page":"e201911035","DOI":"10.1083\/jcb.201911035","article-title":"STRIPAK regulates Slik localization to control mitotic morphogenesis and epithelial integrity","volume":"219","author":"CV De Jamblinne","year":"2020","journal-title":"J Cell Biol"},{"issue":"30","key":"pntd.0013064.ref024","doi-asserted-by":"crossref","first-page":"19108","DOI":"10.1074\/jbc.273.30.19108","article-title":"Dap160, a neural-specific Eps15 homology and multiple SH3 domain-containing protein that interacts with Drosophila dynamin","volume":"273","author":"J Roos","year":"1998","journal-title":"J Biol Chem"},{"issue":"2","key":"pntd.0013064.ref025","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3390\/insects15020084","article-title":"RNAi-Mediated Knockdown of Acidic Ribosomal Stalk Protein P1 Arrests Egg Development in Adult Female Yellow Fever Mosquitoes, Aedes aegypti","volume":"15","author":"M Lamsal","year":"2024","journal-title":"Insects"},{"issue":"6","key":"pntd.0013064.ref026","doi-asserted-by":"crossref","first-page":"950-962.e7","DOI":"10.1016\/j.chom.2020.04.010","article-title":"A Natural Peptide Antigen within the Plasmodium Ribosomal Protein RPL6 Confers Liver TRM Cell-Mediated Immunity against Malaria in Mice","volume":"27","author":"AM Valencia-Hernandez","year":"2020","journal-title":"Cell Host Microbe"},{"key":"pntd.0013064.ref027","doi-asserted-by":"crossref","unstructured":"Xu YJ, Zhang YN, Hao SP, Wang YJ, Yang XX, Shen YQ, et al. Proteotranscriptomic analyses of the midgut and Malpighian tubules after a sublethal concentration of Cry1Ab exposure on Spodoptera litura. Pest management science. 2024;80:2587\u201395.","DOI":"10.1002\/ps.7965"},{"issue":"12","key":"pntd.0013064.ref028","doi-asserted-by":"crossref","first-page":"e3000969","DOI":"10.1371\/journal.pbio.3000969","article-title":"Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes","volume":"18","author":"A Vasquez-Rifo","year":"2020","journal-title":"PLoS Biol"},{"issue":"2","key":"pntd.0013064.ref029","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s13238-016-0303-4","article-title":"The emerging roles of the DDX41 protein in immunity and diseases","volume":"8","author":"Y Jiang","year":"2017","journal-title":"Protein Cell"},{"issue":"12","key":"pntd.0013064.ref030","doi-asserted-by":"crossref","first-page":"6575","DOI":"10.1002\/ps.8396","article-title":"Three RNA helicase DDX genes are essential for the development and oocyte maturation in Laodelphax striatellus","volume":"80","author":"Q-L Ma","year":"2024","journal-title":"Pest Manag Sci"},{"issue":"11","key":"pntd.0013064.ref031","doi-asserted-by":"crossref","first-page":"e1010694","DOI":"10.1371\/journal.ppat.1010694","article-title":"The DEAD-box RNA helicase Dhx15 controls glycolysis and arbovirus replication in Aedes aegypti mosquito cells","volume":"18","author":"S Rosendo Machado","year":"2022","journal-title":"PLoS Pathog"},{"issue":"2","key":"pntd.0013064.ref032","doi-asserted-by":"crossref","first-page":"e00661-21","DOI":"10.1128\/mBio.00661-21","article-title":"Functional Characterization of the m6A-Dependent Translational Modulator PfYTH.2 in the Human Malaria Parasite","volume":"12","author":"A Sinha","year":"2021","journal-title":"mBio"},{"issue":"3","key":"pntd.0013064.ref033","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1038\/s44319-024-00067-z","article-title":"Drosophila eIF3f1 mediates host immune defense by targeting dTak1","volume":"25","author":"Y Hu","year":"2024","journal-title":"EMBO Rep"},{"issue":"5","key":"pntd.0013064.ref034","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1111\/1744-7917.13174","article-title":"eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction, and survival","volume":"30","author":"P Ameijeiras","year":"2023","journal-title":"Insect Sci"},{"issue":"3","key":"pntd.0013064.ref035","doi-asserted-by":"crossref","first-page":"455","DOI":"10.15252\/embr.201541132","article-title":"Life span extension by targeting a link between metabolism and histone acetylation in Drosophila","volume":"17","author":"S Peleg","year":"2016","journal-title":"EMBO Rep"},{"issue":"7","key":"pntd.0013064.ref036","doi-asserted-by":"crossref","first-page":"e1002197","DOI":"10.1371\/journal.pbio.1002197","article-title":"Impaired Mitochondrial Energy Production Causes Light-Induced Photoreceptor Degeneration Independent of Oxidative Stress","volume":"13","author":"M Jaiswal","year":"2015","journal-title":"PLoS Biol"},{"key":"pntd.0013064.ref037","doi-asserted-by":"crossref","first-page":"102932","DOI":"10.1016\/j.redox.2023.102932","article-title":"Defective function of \u03b1-ketoglutarate dehydrogenase exacerbates mitochondrial ATP deficits during complex I deficiency","volume":"67","author":"GG Piroli","year":"2023","journal-title":"Redox Biol"},{"key":"pntd.0013064.ref038","doi-asserted-by":"crossref","first-page":"132454","DOI":"10.1016\/j.foodchem.2022.132454","article-title":"Identification of key genes and enzymes contributing to nutrition conversion of Torreya grandis nuts during post-ripening process","volume":"384","author":"L Song","year":"2022","journal-title":"Food Chem"},{"issue":"Pt B","key":"pntd.0013064.ref039","doi-asserted-by":"crossref","first-page":"105890","DOI":"10.1016\/j.micpath.2022.105890","article-title":"Acetylation of citrate synthase inhibits Bombyx mori nucleopolyhedrovirus propagation by affecting energy metabolism","volume":"173","author":"M Hu","year":"2022","journal-title":"Microb Pathog"},{"issue":"1","key":"pntd.0013064.ref040","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s12864-015-1343-5","article-title":"Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.)","volume":"16","author":"Y You","year":"2015","journal-title":"BMC Genomics"},{"issue":"7","key":"pntd.0013064.ref041","doi-asserted-by":"crossref","first-page":"R132","DOI":"10.1186\/gb-2007-8-7-r132","article-title":"Evolutionary conservation of regulated longevity assurance mechanisms","volume":"8","author":"JJ McElwee","year":"2007","journal-title":"Genome Biol"},{"issue":"6","key":"pntd.0013064.ref042","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1002\/ps.2110","article-title":"Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen)","volume":"67","author":"G Qin","year":"2011","journal-title":"Pest Manag Sci"},{"issue":"1","key":"pntd.0013064.ref043","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cub.2004.12.037","article-title":"Drosophila Lk6 kinase controls phosphorylation of eukaryotic translation initiation factor 4E and promotes normal growth and development","volume":"15","author":"N Arquier","year":"2005","journal-title":"Curr Biol"},{"issue":"4","key":"pntd.0013064.ref044","doi-asserted-by":"crossref","first-page":"dev200477","DOI":"10.1242\/dev.200477","article-title":"Eukaryotic translation initiation factor eIF4E-5 is required for spermiogenesis in Drosophila melanogaster","volume":"150","author":"L Shao","year":"2023","journal-title":"Development"},{"issue":"2","key":"pntd.0013064.ref045","doi-asserted-by":"crossref","first-page":"305","DOI":"10.4067\/S0716-97602002000200022","article-title":"The S6 kinase signaling pathway in the control of development and growth","volume":"35","author":"G Thomas","year":"2002","journal-title":"Biol Res"},{"issue":"5436","key":"pntd.0013064.ref046","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1126\/science.285.5436.2126","article-title":"Drosophila S6 kinase: a regulator of cell size","volume":"285","author":"J Montagne","year":"1999","journal-title":"Science"},{"issue":"1","key":"pntd.0013064.ref047","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1073\/pnas.91.1.316","article-title":"Cysteine-rich LIM domains of LIM-homeodomain and LIM-only proteins contain zinc but not iron","volume":"91","author":"VE Archer","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pntd.0013064.ref048","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1083\/jcb.134.5.1179","article-title":"Two muscle-specific LIM proteins in Drosophila","volume":"134","author":"BE Stronach","year":"1996","journal-title":"J Cell Biol"},{"issue":"3","key":"pntd.0013064.ref049","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S1534-5807(04)00055-3","article-title":"Tensin stabilizes integrin adhesive contacts in Drosophila","volume":"6","author":"CN Torgler","year":"2004","journal-title":"Dev Cell"},{"issue":"6131","key":"pntd.0013064.ref050","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1126\/science.1231031","article-title":"PINK1-phosphorylated mitofusin 2 is a Parkin receptor for culling damaged mitochondria","volume":"340","author":"Y Chen","year":"2013","journal-title":"Science"},{"issue":"8","key":"pntd.0013064.ref051","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1093\/emboj\/16.8.1961","article-title":"The heat shock protein 83 (Hsp83) is required for Raf-mediated signalling in Drosophila","volume":"16","author":"A van der Straten","year":"1997","journal-title":"EMBO J"},{"issue":"9","key":"pntd.0013064.ref052","doi-asserted-by":"crossref","first-page":"3236","DOI":"10.1093\/brain\/awac144","article-title":"Clearance of variant Creutzfeldt-Jakob disease prions in vivo by the Hsp70 disaggregase system","volume":"145","author":"AM Thackray","year":"2022","journal-title":"Brain"},{"issue":"16","key":"pntd.0013064.ref053","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.cub.2008.07.026","article-title":"Costal2 functions as a kinesin-like protein in the hedgehog signal transduction pathway","volume":"18","author":"SF Farzan","year":"2008","journal-title":"Curr Biol"},{"issue":"9","key":"pntd.0013064.ref054","doi-asserted-by":"crossref","first-page":"e201912117","DOI":"10.1083\/jcb.201912117","article-title":"The kinesin-like protein Pavarotti functions noncanonically to regulate actin dynamics","volume":"219","author":"M Nakamura","year":"2020","journal-title":"J Cell Biol"},{"issue":"5","key":"pntd.0013064.ref055","doi-asserted-by":"crossref","first-page":"ENEURO.0110-16.2016","DOI":"10.1523\/ENEURO.0110-16.2016","article-title":"Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury","volume":"3","author":"T Katano","year":"2016","journal-title":"eNeuro"},{"issue":"3","key":"pntd.0013064.ref056","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0092-8674(81)90009-X","article-title":"The discs-large tumor suppressor gene of Drosophila encodes a guanylate kinase homolog localized at septate junctions","volume":"66","author":"DF Woods","year":"1991","journal-title":"Cell"},{"key":"pntd.0013064.ref057","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0048-9697(84)90344-9","article-title":"Glutathione S-transferase in aquatic macro-invertebrates and its interaction with different organic micropollutants","volume":"40","author":"PJ Dierickx","year":"1984","journal-title":"Sci Total Environ"},{"issue":"10","key":"pntd.0013064.ref058","doi-asserted-by":"crossref","first-page":"4127","DOI":"10.1002\/ps.7031","article-title":"Glutathione S-transferase (GST) genes and their function associated with pyrethroid resistance in the malaria vector Anopheles sinensis","volume":"78","author":"F Tao","year":"2022","journal-title":"Pest Manag Sci"},{"key":"pntd.0013064.ref059","doi-asserted-by":"crossref","first-page":"105425","DOI":"10.1016\/j.pestbp.2023.105425","article-title":"Identification of 14 glutathione S-transferase genes from Lasioderma serricorne and characterization of LsGSTe1 involved in lambda-cyhalothrin detoxification","volume":"193","author":"X-Y Liu","year":"2023","journal-title":"Pestic Biochem Physiol"},{"key":"pntd.0013064.ref060","doi-asserted-by":"crossref","first-page":"105498","DOI":"10.1016\/j.pestbp.2023.105498","article-title":"Functional analysis of an overexpressed glutathione S-transferase BdGSTd5 involved in malathion and malaoxon detoxification in Bactrocera dorsalis","volume":"194","author":"S Zhang","year":"2023","journal-title":"Pestic Biochem Physiol"},{"issue":"2","key":"pntd.0013064.ref061","doi-asserted-by":"crossref","first-page":"e0005313","DOI":"10.1371\/journal.pntd.0005313","article-title":"Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families","volume":"11","author":"L Traverso","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0013064.ref062","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1080\/21505594.2017.1370174","article-title":"Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans","volume":"9","author":"G Sheehan","year":"2018","journal-title":"Virulence"},{"key":"pntd.0013064.ref063","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ibmb.2015.10.005","article-title":"Glutathione S-transferase SlGSTE1 in Spodoptera litura may be associated with feeding adaptation of host plants","volume":"70","author":"X Zou","year":"2016","journal-title":"Insect Biochem Mol Biol"},{"issue":"3","key":"pntd.0013064.ref064","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1002\/ps.4747","article-title":"RNA interference of two glutathione S-transferase genes, Diaphorina citri DcGSTe2 and DcGSTd1, increases the susceptibility of Asian citrus psyllid (Hemiptera: Liviidae) to the pesticides fenpropathrin and thiamethoxam","volume":"74","author":"X Yu","year":"2018","journal-title":"Pest Manag Sci"},{"issue":"1","key":"pntd.0013064.ref065","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/imb.12812","article-title":"Myofilaments promote wing expansion and maintain genitalia morphology in the American cockroach, Periplaneta americana","volume":"32","author":"F Liu","year":"2023","journal-title":"Insect Mol Biol"},{"key":"pntd.0013064.ref066","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.jinsphys.2019.02.012","article-title":"Potential roles of insect Tropomyosin1-X1 isoform in the process of Candidatus Liberibacter asiaticus infection of Diaphorina citri","volume":"114","author":"Z-J Lu","year":"2019","journal-title":"J Insect Physiol"},{"issue":"12","key":"pntd.0013064.ref067","doi-asserted-by":"crossref","first-page":"e0012618","DOI":"10.1371\/journal.pntd.0012618","article-title":"Research progress toward arthropod salivary protein vaccine development for vector-borne infectious diseases","volume":"18","author":"Y Wang","year":"2024","journal-title":"PLoS Negl Trop Dis"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T17:43:36Z","timestamp":1746639816000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013064"}},"subtitle":[],"editor":[{"given":"Travis J","family":"Bourret","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,7]]},"references-count":67,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0013064","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,7]]}}}