{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:26:59Z","timestamp":1789900019466,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013069","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000}}],"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260412"],"award-info":[{"award-number":["82260412"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High-end Innovation of Qinghai Province","award":["Thousand Talents Program"],"award-info":[{"award-number":["Thousand Talents Program"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To develop a novel non-invasive CRISPR\/Cas12a-RCA assay for the primary screening of human\n                      <jats:italic>echinococcosis<\/jats:italic>\n                      via detection of circulating\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      cell-free DNA (cfDNA) in peripheral blood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Plasma cfDNA from 20 AE patients was analyzed via high-throughput sequencing to identify conserved repetitive\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      fragments.A one-pot RCA system coupled with CRISPR\/Cas12a was optimized for\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      -cfDNA detection. The limit of detection (LOD) was determined using serially diluted synthetic standards, while specificity was validated through mismatch probes and cross-reactivity testing. Clinical validation included 50 AE cases, 22 cystic echinococcosis (CE) cases, 43 non-\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      hepatic disease (HD) cases, and 53 healthy controls (CON).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A conserved repetitive 28S rDNA fragment (pan-\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      -28S) was identified as a biomarker. The CRISPR\/Cas12a-RCA assay achieved amplification within 30 minutes at 37 \u00b0C, with a linear range of 1 aM to 100 pM and an LOD of 1.41 aM. Visual detection limits were 10 aM (UV light) and 1 aM (blue light). The assay demonstrated high sensitivity (87.5%) and specificity (96.9%, AUC\u2009=\u20090.933) in distinguishing\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      infection (AE\/CE) from HD and CON.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The optimized one-pot CRISPR\/Cas12a-RCA system enables rapid and ultrasensitive detection of pan-\n                      <jats:italic>Echinococcus<\/jats:italic>\n                      cfDNA, providing a cost-effective and highly accurate solution for the primary screening of echinococcosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0013069","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:38:05Z","timestamp":1767897485000},"page":"e0013069","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["CRISPR\/Cas12a-RCA enables ultrasensitive detection of circulating free DNA for noninvasive diagnosis of echinococcosis"],"prefix":"10.1371","volume":"20","author":[{"given":"Jideng","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanmin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jide","family":"A","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0031-8951","authenticated-orcid":true,"given":"Lei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7645-1497","authenticated-orcid":true,"given":"Xiangren","family":"A","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"pntd.0013069.ref001","article-title":"Renal echinococcosis; the parasite, host immune response, diagnosis and management","author":"YD H","year":"2020","journal-title":"Journal of Infection in Developing Countries"},{"issue":"2","key":"pntd.0013069.ref002","article-title":"Echinococcosis: advances in the 21st century","volume":"32","author":"H Wen","year":"2019","journal-title":"Clin Microbiol Rev"},{"key":"pntd.0013069.ref003","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/bs.apar.2016.09.002","article-title":"Echinococcosis: Control and Prevention","volume":"96","author":"PS Craig","year":"2017","journal-title":"Adv Parasitol"},{"key":"pntd.0013069.ref004","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/bs.apar.2016.11.001","article-title":"Global Distribution of Alveolar and Cystic Echinococcosis","volume":"95","author":"P Deplazes","year":"2017","journal-title":"Adv Parasitol"},{"issue":"5","key":"pntd.0013069.ref005","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/s00436-003-0903-9","article-title":"Epidemiology, clinical manifestations and diagnosis of zoonotic cestode infections: an update","volume":"91","author":"W Raether","year":"2003","journal-title":"Parasitol Res"},{"issue":"4","key":"pntd.0013069.ref006","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.crad.2019.01.007","article-title":"Hepatic alveolar hydatid disease (Echinococcus multilocularis), a mimic of liver malignancy: a review for the radiologist in non-endemic areas","volume":"74","author":"MD Chouhan","year":"2019","journal-title":"Clin Radiol"},{"issue":"1","key":"pntd.0013069.ref007","doi-asserted-by":"crossref","first-page":"43","DOI":"10.3892\/etm.2015.2857","article-title":"Analysis of the clinical value of 18F-FDG PET\/CT in hepatic alveolar echinococcosis before and after autologous liver transplantation","volume":"11","author":"Y Qin","year":"2016","journal-title":"Exp Ther Med"},{"key":"pntd.0013069.ref008","article-title":"Immunology of cystic echinococcosis (hydatid disease)","author":"D \u00c1","year":"2017","journal-title":"British medical bulletin"},{"issue":"1","key":"pntd.0013069.ref009","doi-asserted-by":"crossref","first-page":"23","DOI":"10.4269\/ajtmh.2002.66.23","article-title":"Combined ultrasound and serologic screening for hepatic alveolar echinococcosis in central China","volume":"66","author":"G Bartholomot","year":"2002","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"pntd.0013069.ref010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2009.11.001","article-title":"Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans","volume":"114","author":"Writing Panel for the WHO-IWGE","year":"2010","journal-title":"Acta Trop"},{"issue":"6","key":"pntd.0013069.ref011","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S1473-3099(07)70134-2","article-title":"Prevention and control of cystic echinococcosis","volume":"7","author":"PS Craig","year":"2007","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"pntd.0013069.ref012","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/s13071-019-3569-6","article-title":"Survey of echinococcoses in southeastern Qinghai Province, China, and serodiagnostic insights of recombinant Echinococcus granulosus antigen B isoforms","volume":"12","author":"X Han","year":"2019","journal-title":"Parasit Vectors"},{"issue":"3","key":"pntd.0013069.ref013","doi-asserted-by":"crossref","first-page":"730","DOI":"10.3390\/ijms21030730","article-title":"MiRNAs and lncRNAs in Echinococcus and Echinococcosis","volume":"21","author":"H Z","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"pntd.0013069.ref014","first-page":"00168","article-title":"Cell-free DNA: a promising biomarker in infectious diseases","author":"Y Hu","year":"2024","journal-title":"Trends in Microbiology"},{"issue":"1","key":"pntd.0013069.ref015","article-title":"Parasite Circulating Cell-free DNA in the Blood of Alveolar Echinococcosis Patients as a Diagnostic and Treatment-Status Indicator","volume":"73","author":"H Fan","year":"2021","journal-title":"Clin Infect Dis"},{"issue":"2","key":"pntd.0013069.ref016","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.ygeno.2020.12.035","article-title":"Characterizing dynamic changes of plasma cell-free Echinococcus granulosus DNA before and after cystic echinococcosis treatment initiation","volume":"113","author":"Y Zhao","year":"2021","journal-title":"Genomics"},{"key":"pntd.0013069.ref017","doi-asserted-by":"crossref","first-page":"1413532","DOI":"10.3389\/fmicb.2024.1413532","article-title":"Circulating free DNA as a diagnostic marker for echinococcosis: a systematic review and meta-analysis","volume":"15","author":"X Luo","year":"2024","journal-title":"Front Microbiol"},{"issue":"7","key":"pntd.0013069.ref018","doi-asserted-by":"crossref","DOI":"10.1016\/S2666-5247(22)00087-8","article-title":"CRISPR detection of circulating cell-free Mycobacterium tuberculosis DNA in adults and children, including children with HIV: a molecular diagnostics study","volume":"3","author":"Z Huang","year":"2022","journal-title":"Lancet Microbe"},{"key":"pntd.0013069.ref019","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1186\/1756-3305-7-254","article-title":"Loop-mediated isothermal amplification (LAMP) assay for the identification of Echinococcus multilocularis infections in canine definitive hosts","volume":"7","author":"X Ni","year":"2014","journal-title":"Parasit Vectors"},{"issue":"5","key":"pntd.0013069.ref020","doi-asserted-by":"crossref","first-page":"883","DOI":"10.4269\/ajtmh.2012.12-0184","article-title":"The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosus [corrected] coprodetection","volume":"87","author":"H Salant","year":"2012","journal-title":"Am J Trop Med Hyg"},{"issue":"7","key":"pntd.0013069.ref021","article-title":"A comparison of loop-mediated isothermal amplification (LAMP) with other surveillance tools for Echinococcus granulosus diagnosis in canine definitive hosts","volume":"9","author":"X-W Ni","year":"2014","journal-title":"PLoS One"},{"issue":"2","key":"pntd.0013069.ref022","first-page":"168","article-title":"Establishment of a recombinase-aided isothermal amplification assay for nucleic acid detection of Echinococcus multilocularis and its preliminary application","volume":"32","author":"HR Zhou","year":"2020","journal-title":"Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi"},{"issue":"1","key":"pntd.0013069.ref023","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1038\/s41467-023-37183-8","article-title":"Genome-wide tiled detection of circulating Mycobacterium tuberculosis cell-free DNA using Cas13","volume":"14","author":"SG Thakku","year":"2023","journal-title":"Nat Commun"},{"issue":"1","key":"pntd.0013069.ref024","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s13071-024-06134-7","article-title":"Ov-RPA-CRISPR\/Cas12a assay for the detection of Opisthorchis viverrini infection in field-collected human feces","volume":"17","author":"O Phuphisut","year":"2024","journal-title":"Parasit Vectors"},{"key":"pntd.0013069.ref025","doi-asserted-by":"crossref","first-page":"124582","DOI":"10.1016\/j.talanta.2023.124582","article-title":"CRISPR\/Cas13a-triggered Cas12a biosensing method for ultrasensitive and specific miRNA detection","volume":"260","author":"D Zhao","year":"2023","journal-title":"Talanta"},{"issue":"24","key":"pntd.0013069.ref026","doi-asserted-by":"crossref","first-page":"28933","DOI":"10.1021\/acsami.3c03399","article-title":"Ultrasensitive Detection of miRNA via CRISPR\/Cas12a Coupled with Strand Displacement Amplification Reaction","volume":"15","author":"S Feng","year":"2023","journal-title":"ACS Appl Mater Interfaces"},{"issue":"11","key":"pntd.0013069.ref027","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00782-19","article-title":"Investigation of Preanalytical Variables Impacting Pathogen Cell-Free DNA in Blood and Urine","volume":"57","author":"K Murugesan","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"12","key":"pntd.0013069.ref028","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1038\/s41551-023-01033-1","article-title":"A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs","volume":"7","author":"H Yan","year":"2023","journal-title":"Nat Biomed Eng"},{"issue":"6","key":"pntd.0013069.ref029","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s13205-023-03628-6","article-title":"Isothermal nucleic acid amplification and its uses in modern diagnostic technologies","volume":"13","author":"P Srivastava","year":"2023","journal-title":"3 Biotech"},{"key":"pntd.0013069.ref030","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.343593","article-title":"A miniaturized RPA-CRISPR\/Cas12a-based nucleic acid diagnostic platform for rapid and simple self-testing of SARS-CoV-2","volume":"1338","author":"T Zhang","year":"2025","journal-title":"Analytica Chimica Acta"},{"issue":"40","key":"pntd.0013069.ref031","doi-asserted-by":"crossref","first-page":"15998","DOI":"10.1021\/acs.analchem.4c03450","article-title":"CRISPR-Responsive RCA-Based DNA Hydrogel Biosensing Platform with Customizable Signal Output for Rapid and Sensitive Nucleic Acid Detection","volume":"96","author":"Y Zhang","year":"2024","journal-title":"Anal Chem"},{"issue":"17","key":"pntd.0013069.ref032","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"S Chen","year":"2018","journal-title":"Bioinformatics"},{"issue":"4","key":"pntd.0013069.ref033","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"pntd.0013069.ref034","first-page":"1","article-title":"MicroRNA sensors based on CRISPR\/Cas12a technologies: evolution from indirect to direct detection","author":"S Yu","year":"2024","journal-title":"Critical Reviews in Analytical Chemistry"},{"key":"pntd.0013069.ref035","first-page":"1","article-title":"Amplification-free nucleic acids detection with next-generation CRISPR\/dx systems","author":"CH Lau","year":"2024","journal-title":"Crit Rev Biotechnol"},{"key":"pntd.0013069.ref036","doi-asserted-by":"crossref","first-page":"115248","DOI":"10.1016\/j.bios.2023.115248","article-title":"An aM-level sensitive cascade CRISPR-Dx system (ASCas) for rapid detection of RNA without pre-amplification","volume":"230","author":"Y Zhang","year":"2023","journal-title":"Biosens Bioelectron"},{"issue":"6","key":"pntd.0013069.ref037","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1007\/s00216-020-03121-6","article-title":"Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155","volume":"413","author":"Y Zheng","year":"2021","journal-title":"Anal Bioanal Chem"},{"issue":"43","key":"pntd.0013069.ref038","doi-asserted-by":"crossref","first-page":"17362","DOI":"10.1021\/acs.analchem.4c04067","article-title":"Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR\/Cas Digital Imaging Platform","volume":"96","author":"Y Li","year":"2024","journal-title":"Anal Chem"},{"issue":"2","key":"pntd.0013069.ref039","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/14737159.2023.2178903","article-title":"Diagnosis of echinococcosis by detecting circulating cell-free DNA and miRNA","volume":"23","author":"M Hadipour","year":"2023","journal-title":"Expert Rev Mol Diagn"},{"issue":"1","key":"pntd.0013069.ref040","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1007\/s11033-024-09385-w","article-title":"Detection of circulatory E. granulosus-derived cell-free DNA in the plasma and urine of human cystic echinococcosis using an in-house PCR: a potential promising diagnostic biomarker","volume":"51","author":"B Habibi","year":"2024","journal-title":"Mol Biol Rep"},{"key":"pntd.0013069.ref041","doi-asserted-by":"crossref","first-page":"111314","DOI":"10.1016\/j.molbiopara.2020.111314","article-title":"Diagnostic urinary cfDNA detected in human cystic echinococcosis","volume":"239","author":"L Toribio","year":"2020","journal-title":"Mol Biochem Parasitol"},{"key":"pntd.0013069.ref042","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.molbiopara.2018.04.004","article-title":"Circulating cell-free DNA in patients with alveolar echinococcosis","volume":"222","author":"A Baraquin","year":"2018","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"pntd.0013069.ref043","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ajhg.2020.01.008","article-title":"The Biology of Cell-free DNA Fragmentation and the Roles of DNASE1, DNASE1L3, and DFFB","volume":"106","author":"DSC Han","year":"2020","journal-title":"Am J Hum Genet"},{"issue":"10","key":"pntd.0013069.ref044","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011715","article-title":"Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis","volume":"17","author":"M Hadipour","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013069.ref045","doi-asserted-by":"crossref","first-page":"110276","DOI":"10.1016\/j.vetpar.2024.110276","article-title":"Optimization of CRISPR\/Cas12a detection assay and its application in the detection of Echinococcus granulosus","volume":"331","author":"F Huang","year":"2024","journal-title":"Vet Parasitol"},{"issue":"2","key":"pntd.0013069.ref046","doi-asserted-by":"crossref","first-page":"2176","DOI":"10.1021\/acs.analchem.9b04814","article-title":"Rolling Circular Amplification (RCA)-Assisted CRISPR\/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs","volume":"92","author":"R Wang","year":"2020","journal-title":"Anal Chem"},{"issue":"46","key":"pntd.0013069.ref047","doi-asserted-by":"crossref","first-page":"44030","DOI":"10.1021\/acsomega.3c06227","article-title":"Hairpin\/DNA Ring Ternary Complex Initiated Rolling Circle Amplification for an Elevated Accuracy and Its Application in Analyzing Let-7a","volume":"8","author":"H Hui","year":"2023","journal-title":"ACS Omega"},{"issue":"1","key":"pntd.0013069.ref048","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40249-021-00807-6","article-title":"Dynamic modeling and optimal control of cystic echinococcosis","volume":"10","author":"X Rong","year":"2021","journal-title":"Infect Dis Poverty"},{"issue":"20","key":"pntd.0013069.ref049","doi-asserted-by":"crossref","first-page":"3256","DOI":"10.1039\/D4AY00693C","article-title":"A one-pot CRISPR-RCA strategy for ultrasensitive and specific detection of circRNA","volume":"16","author":"X Ke","year":"2024","journal-title":"Anal Methods"},{"issue":"15","key":"pntd.0013069.ref050","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202309534","article-title":"A DNA\/Upconversion Nanoparticle Complex Enables Controlled Co-Delivery of CRISPR-Cas9 and Photodynamic Agents for Synergistic Cancer Therapy","volume":"36","author":"N Song","year":"2024","journal-title":"Adv Mater"},{"issue":"12","key":"pntd.0013069.ref051","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0012753","article-title":"Development of a Copro-RPA-CRISPR\/Cas12a assay to detect Echinococcus granulosus nucleic acids isolated from canine feces using NaOH-based DNA extraction method","volume":"18","author":"G Shao","year":"2024","journal-title":"PLoS Negl Trop Dis"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0013069","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T18:59:16Z","timestamp":1768589956000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013069"}},"subtitle":[],"editor":[{"given":"Qiulun","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0013069","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]}}}