{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T02:43:03Z","timestamp":1772678583987,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013099","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000}}],"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"State of New York"},{"name":"National Institute of Health","award":["R01AI175941"],"award-info":[{"award-number":["R01AI175941"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    mosquitoes transmit several arboviruses of public health importance. Among these is dengue virus (DENV), a flavivirus whose global infection rates continue to rise each year. With limited options available for preventing or treating DENV infections, mosquito control remains the most widely implemented strategy to combat DENV transmission. Due to the global distribution of DENV, which infects an estimated 400 million people per year, vector suppression practices vary drastically by country and\/or region and even small differences in microenvironment can significantly impact vector abundance. There remains a significant need to better understand vector exposure rates at an individual level to disentangle vector exposure and arboviral infection rates. To this end, we have optimized a serologic assay to assess the abundance of antibodies directed against the mosquito salivary proteins AeD7L1\u2009+\u20092 as a surrogate metric of vector exposure. Utilizing this assay, we found that anti-AeD7L1\u2009+\u20092 IgG levels were unable to identify low levels of\n                    <jats:italic>Aedes<\/jats:italic>\n                    exposure in individuals with limited prior\n                    <jats:italic>Aedes<\/jats:italic>\n                    exposure, indicating they are unreliable markers of an individual\u2019s recent exposure to low levels of these vectors. However, antibody levels against AeD7L1\u2009+\u20092 were robust in plasma samples from individuals living in\n                    <jats:italic>Aedes<\/jats:italic>\n                    endemic regions. These antibody levels reflected seasonal changes in\n                    <jats:italic>Aedes<\/jats:italic>\n                    abundance and exposure, indicating their potential for use as an aggregate marker of vector exposure. Additionally, we found that there were negative associations with anti-AeD7L1\u2009+\u20092 IgG levels and age in our cohort. Interestingly, we also found that lower titers of anti-AeD7L1\u2009+\u20092 IgG correlated with higher infection burden in households. This finding has implications for the potential interaction between AeD7L1\u2009+\u20092 proteins or anti-AeD7L1\u2009+\u20092 antibodies and DENV during infection events that will require further study.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013099","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T17:47:35Z","timestamp":1761760055000},"page":"e0013099","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antibody response to Aedes aegypti D7L1\u2009+\u20092 salivary proteins as marker of aggregate vector exposure and correlate of dengue virus susceptibility"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0080-3934","authenticated-orcid":true,"given":"Lauren E.","family":"Bahr","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Hamins-Puertolas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darunee","family":"Buddhari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivona","family":"Petzlova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saravanan","family":"Thangamani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4536-6714","authenticated-orcid":true,"given":"Adam T.","family":"Waickman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn B.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"issue":"7446","key":"pntd.0013099.ref001","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"issue":"4","key":"pntd.0013099.ref002","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005568","article-title":"Detecting the impact of temperature on transmission of Zika, dengue, and chikungunya using mechanistic models","volume":"11","author":"EA Mordecai","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013099.ref003","article-title":"Climate warming is expanding dengue burden in the Americas and Asia","author":"ML Childs","year":"2025","journal-title":"medRxiv"},{"key":"pntd.0013099.ref004","doi-asserted-by":"crossref","first-page":"100712","DOI":"10.1016\/j.eclinm.2020.100712","article-title":"Global, regional, and national dengue burden from 1990 to 2017: A systematic analysis based on the global burden of disease study 2017","volume":"32","author":"Z Zeng","year":"2021","journal-title":"EClinicalMedicine"},{"key":"pntd.0013099.ref005","unstructured":"World Health Organization. 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