{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T23:34:09Z","timestamp":1768779249462,"version":"3.49.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Research Development Program of China","award":["2022YFC2305000, 2022YFC2305003"],"award-info":[{"award-number":["2022YFC2305000, 2022YFC2305003"]}]},{"DOI":"10.13039\/501100010834","name":"Education Department of Hainan Province","doi-asserted-by":"publisher","award":["Qhys2023-461"],"award-info":[{"award-number":["Qhys2023-461"]}],"id":[{"id":"10.13039\/501100010834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370018"],"award-info":[{"award-number":["82370018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460414"],"award-info":[{"award-number":["82460414"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Melioidosis, caused by <jats:italic>Burkholderia pseudomallei<\/jats:italic>, is a tropical disease known for its long incubation period and high mortality rate. However, the traits of this \u201cgreat imitator\u201d present significant challenges for clinical diagnosis and pose a serious threat to populations in epidemic regions. A rapid, accurate, and low environment requirement diagnostic method is needed to enable timely diagnoses. Here, we developed a strategy for point-of-care testing (POCT) using loop-mediated isothermal amplification (LAMP) with RNase HII for efficient detection of <jats:italic>B. pseudomallei<\/jats:italic>, through real-time fluorescence analysis or lateral flow strip assay (LFSA). Briefly, RNase HII-based cleavage dual-functional probe LAMP (RCDP-LAMP) utilizes RNase HII cleave the \u201c<jats:italic>rG<\/jats:italic>\u201d from the probe which hybridized with target. FAM group was cleaved, detaching from BHQ1, and exciting fluorescence or Biotin group on the same side as BHQ1 was used as the target captured in LFSA for the results. The limit of detection (LOD) for is 50 fg\/reaction. In summary, RCDP-LAMP presents a versatile tool for detecting <jats:italic>B. pseudomallei<\/jats:italic> in both professional clinical laboratories and POCT scenes, suitable for large-scale screening efforts. This approach is essential for controlling costs in primary healthcare units and significantly enhances the prevention and management of the severely neglected tropical disease.<\/jats:p>","DOI":"10.1371\/journal.pntd.0013109","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T17:49:19Z","timestamp":1749059359000},"page":"e0013109","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis"],"prefix":"10.1371","volume":"19","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuqiong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5521-5664","authenticated-orcid":true,"given":"Qianfeng","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,6,4]]},"reference":[{"key":"pntd.0013109.ref001","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1099\/00207713-48-1-317","article-title":"Burkholderia 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Yao","year":"2023","journal-title":"Microbiol Spectr"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T17:49:27Z","timestamp":1749059367000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013109"}},"subtitle":[],"editor":[{"given":"Joseph 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