{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T16:54:02Z","timestamp":1782752042508,"version":"3.54.5"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T00:00:00Z","timestamp":1753228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Henan Province\u2019s International Scientific and Technological Cooperation Project","award":["242102521041"],"award-info":[{"award-number":["242102521041"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is an intracellular protozoan whose intermediate hosts encompass nearly all warm-blooded animals, including humans and non-human primates (NHPs). In this study, molecular, serological, immunohistochemical and bioassay methods were used to investigate <jats:italic>T. gondii<\/jats:italic> infection in 17 captived NHPs in zoos from China between 2022 and 2023. The infection rate was higher in New World NHPs (83.3%) than in Old World NHPs (16.7%) (<jats:italic>P\u2009<\/jats:italic>=\u20090.2424). A viable strain of <jats:italic>T. gondii<\/jats:italic> was successfully isolated from the tissues of a black mangabey (<jats:italic>Lophocebus aterrimus<\/jats:italic>). The strain designated as TgMonkeyCHn3 was genotyped as ToxoDB #6, which had demonstrated virulence in Swiss Webster outbred mice. The ROP18\/ROP5 allele combination was identified as 1\/3. The emergence of this strain highlights the increased genetic diversity of <jats:italic>T. gondii<\/jats:italic> in East Asia and presents new challenges for the prevention of toxoplasmosis, particularly in captive NHPs. To the best of our knowledge, this is the first report of the ToxoDB genotype #6 isolated in East Asia and the black mangabey is a new host for <jats:italic>T. gondii<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pntd.0013133","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T17:31:51Z","timestamp":1753291911000},"page":"e0013133","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["First isolation of viable Toxoplasma gondii from a black mangabey (Lophocebus aterrimus) reveals the emergence of the Africa 1 lineage in East 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