{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T18:45:56Z","timestamp":1775587556806,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013236","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000}}],"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-IAHU-03 (M\u00e9diterran\u00e9e Infection)"],"award-info":[{"award-number":["ANR-10-IAHU-03 (M\u00e9diterran\u00e9e Infection)"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010076","name":"Conseil R\u00c3\u00a9gional Provence-Alpes-C\u00c3\u00b4te d'Azur","doi-asserted-by":"publisher","award":["FEDER IHUPERF"],"award-info":[{"award-number":["FEDER IHUPERF"]}],"id":[{"id":"10.13039\/501100010076","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institut de Recherche pour le D\u00c3\u00a9veloppement","award":["MINES"],"award-info":[{"award-number":["MINES"]}]},{"DOI":"10.13039\/100007356","name":"Fondation M\u00c3\u00a9diterran\u00c3\u00a9e Infection","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007356","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    This study explores the use of Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS) to identify and differentiate\n                    <jats:italic>Biomphalaria<\/jats:italic>\n                    snails infected with the parasite\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    , which causes schistosomiasis. The study was conducted on two snail species,\n                    <jats:italic>Biomphalaria pfeifferi<\/jats:italic>\n                    (collected in the field in Senegal) and\n                    <jats:italic>Biomphalaria glabrata<\/jats:italic>\n                    (a laboratory strain). The snails were infected in the laboratory with\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    miracidia, and their infection was confirmed by cercariae emission tests and quantitative PCR (qPCR). MALDI-TOF MS was then used to analyse proteins from infected and uninfected snails to identify spectral differences. Based on protein profiles, the results of MALDI-TOF mass spectrometry made it possible to accurately differentiate between S. mansoni-infected snails and uninfected snails. An increase in the number of peaks detected and their intensity was observed for the spectra of\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    -infected snails compared to uninfected snails. The application of principal component analysis (PCA) to these mass spectrometry profiles confirmed the discrimination between the two groups according to their infection status. In addition, specific discriminating peaks were identified for each snail species, allowing for the distinction of infected from uninfected snails. The present study revealed, for the first time, that MALDI-TOF MS appears to be a rapid, reliable, and specific tool for the diagnosis of schistosomiasis in snails, offering promising prospects for the surveillance and control of this disease in endemic areas. However, further work is needed to establish a MALDI-TOF MS reference spectra database specific to\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    parasites and to standardise sample collection, storage, and preparation in order to apply this technique in the field.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013236","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T17:40:32Z","timestamp":1774374032000},"page":"e0013236","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid identification of Biomphalaria spp. and diagnosis of Schistosoma mansoni infestation using MALDI-TOF mass spectrometry"],"prefix":"10.1371","volume":"20","author":[{"given":"Diara","family":"Sy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lionel","family":"Almeras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adama Zan","family":"Diarra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souleymane","family":"Doucoure","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yacine Mbere","family":"Sarr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"L\u2019Ollivier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Papa Mouhamadou","family":"Gaye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"El Hadji Ibrahima","family":"Ndiaye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Senghor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doudou","family":"Sow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheikh","family":"Sokhna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":true,"given":"Stephane","family":"Ranque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"issue":"9","key":"pntd.0013236.ref001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009669","article-title":"Trends in the prevalence of microscopically-confirmed schistosomiasis in the South African public health sector, 2011-2018","volume":"15","author":"L De Boni","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013236.ref002","doi-asserted-by":"crossref","first-page":"1088981","DOI":"10.3389\/fvets.2023.1088981","article-title":"Knowledge, attitudes and practices related to neglected tropical diseases (schistosomiasis and fascioliasis) of public health importance: a cross-sectional study","volume":"10","author":"S Riaz","year":"2023","journal-title":"Front Vet Sci"},{"issue":"2","key":"pntd.0013236.ref003","doi-asserted-by":"crossref","first-page":"224","DOI":"10.3390\/pathogens11020224","article-title":"Development of new technologies for risk identification of schistosomiasis transmission in China","volume":"11","author":"L Shi","year":"2022","journal-title":"Pathogens"},{"issue":"1","key":"pntd.0013236.ref004","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s40249-017-0256-8","article-title":"Moving from control to elimination of schistosomiasis in sub-Saharan Africa: time to change and adapt strategies","volume":"6","author":"L-A Tchuem Tchuent\u00e9","year":"2017","journal-title":"Infect Dis Poverty"},{"key":"pntd.0013236.ref005","doi-asserted-by":"crossref","unstructured":"Dreyfuss G, Rondelaud D. 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