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The limited identification and validation of schistosome antigens hinders the progress of vaccine development for the disease. Schistosome cysteine proteinases are considered as important targets for novel anti-schistosomiasis immunoprophylaxis due to their primary role in nutrient absorption. Previous research on the <jats:italic>Schistosoma japonicum<\/jats:italic> degradome has identified a group of cathepsin L-like proteases (SjCLs) that are up-regulated in hepatic schistosomula and adult worms.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods\/findings<\/jats:title>\n<jats:p>In this study, five recombinant proteins representing the mature form of these SjCLs, designated as rSjCL1\u20135, were successfully produced. Mice immunized with the rSjCLs were subsequently challenged with cercariae to evaluate the immunoprotective efficacy of these proteins. The expression and localization of SjCL1 were analyzed by qRT-PCR, western blotting and immunofluorescence assays. Among these five rSjCLs, only the immunization with rSjCL1 conferred partial protection to the mice against <jats:italic>S. japonicum<\/jats:italic> infection, resulting in a reduction in worm burden by 34.9%\u2009~\u200938.0% and a decrease of egg burden by 46.2%\u2009~\u200948.3%. This immunization also effectively mitigated body weight loss and hepatomegaly in the challenged mice. SjCL1 was primarily localized along the intestinal intima of hepatic schistosomula, as well as male and female adults, and on the tegument of male adults. The mature form of SjCL1 was detected in the excretory\/secretory products of the parasites. Hepatic schistosomulum treated with SjCL1 antibodies <jats:italic>in vitro<\/jats:italic> showed significant growth retardation, although remained viable and developed intestinal heme pigmentation, indicative of hemoglobin digestion.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/significance<\/jats:title>\n<jats:p>Our study revealed that SjCL1 is essential for normal parasite growth and shed new light for the development of schistosomiasis vaccines targeting cathepsins, which play a key role in the early intra-mammalian stages of schistosomes.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0013241","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T13:52:52Z","timestamp":1751896372000},"page":"e0013241","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis 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