{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:52:52Z","timestamp":1790131972398,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013255","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000}}],"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008551","name":"Fondation M\u00e9rieux","doi-asserted-by":"publisher","award":["RESIS-ARBO1-FM-18"],"award-info":[{"award-number":["RESIS-ARBO1-FM-18"]}],"id":[{"id":"10.13039\/501100008551","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fond Solidarit\u00e9 Sant\u00e9 Navale","award":["RESIS-ARBO2-FSSN-21"],"award-info":[{"award-number":["RESIS-ARBO2-FSSN-21"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec022\">\n<jats:title>Background<\/jats:title>\n<jats:p>Over the last ten years, there has been an upsurge of dengue outbreaks in C\u00f4te d\u2019Ivoire, with <jats:italic>Aedes aegypti<\/jats:italic> as the principal vector. The intensification of farming activities and the living conditions of local populations could lead to a proliferation of artificial breeding sites for <jats:italic>Ae. aegypti<\/jats:italic>, which would increase the risk of dengue transmission in rural areas. The present study characterised the habitats of <jats:italic>Ae. aegypti<\/jats:italic> larvae and assessed the risk of dengue transmission in three key agricultural areas of C\u00f4te d\u2019Ivoire.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec023\">\n<jats:title>Methodology<\/jats:title>\n<jats:p><jats:italic>Aedes aegypti<\/jats:italic> (larvae, pupae and adults) were collected from human dwellings in three key agricultural areas of C\u00f4te d\u2019Ivoire during the rainy season. Risk indices including traditional Stegomyia indices and pupal indices were estimated. RT-qPCR was used to detect DENV in the pools of <jats:italic>Ae. aegypti<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec024\">\n<jats:title>Principal Findings<\/jats:title>\n<jats:p><jats:italic>Aedes aegypti<\/jats:italic> was the predominant species collected at the three locations. The predominant breeding sites were discarded tanks in Songon-Agban and Tchanct\u00e9v\u00e8, and water storage tanks in Kaforo. High Stegomyia indices (house indices &gt;5% and\/or Breteau indices &gt;20) and pupal indices (PIH [0.8 - 2.2] and PIP [0.1 - 0.4]) were recorded in all three sites, suggesting a high risk of dengue transmission. DENV-3 was detected in 1\/96 (3.6%) pools of <jats:italic>Ae. aegypti<\/jats:italic> collected as immature stages in Songon-Agban with a minimum infection rate (MIR) of 1.9 per 1000 mosquitoes.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec025\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>The findings of this study indicated a high entomological risk of dengue across the three agricultural sites. It is important that the potential for transovarial transmission of DENV-3 in <jats:italic>Ae. aegypti<\/jats:italic> is considered when formulating control strategies against this vector in C\u00f4te d\u2019Ivoire.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0013255","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T16:01:46Z","timestamp":1751558506000},"page":"e0013255","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aedes aegypti ecology and dengue infection in three agricultural areas of C\u00f4te d\u2019Ivoire"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7132-7163","authenticated-orcid":true,"given":"Edwige M. A. 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