{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:50:38Z","timestamp":1791399038229,"version":"4.3.3"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013299","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000}}],"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/21354-1"],"award-info":[{"award-number":["2019\/21354-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/08391-0"],"award-info":[{"award-number":["2023\/08391-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/03159-2"],"award-info":[{"award-number":["2023\/03159-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/05426-0"],"award-info":[{"award-number":["2024\/05426-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/14470-3"],"award-info":[{"award-number":["2024\/14470-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/12938-0"],"award-info":[{"award-number":["2021\/12938-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/14432-3"],"award-info":[{"award-number":["2018\/14432-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/00694-6"],"award-info":[{"award-number":["2020\/00694-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/07467-1"],"award-info":[{"award-number":["2013\/07467-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/15553-9"],"award-info":[{"award-number":["2018\/15553-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/02275-1"],"award-info":[{"award-number":["2024\/02275-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    , the etiological agent of Chagas Disease (CD), represents a significant public health concern and serves as a valuable model for investigating the cell cycle in early-diverging eukaryotes. Its unique cellular features,including the absence of chromosome condensation and the coordination of nuclear division with specialized organelles, provide insights into non-canonical regulatory mechanisms, and underscore the pivotal role of the nucleus in maintaining genomic integrity and regulating fundamental processes such as DNA replication and gene expression. To investigate nuclear dynamics throughout the cell cycle, we synchronized\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    epimastigotes at the G1\/S transition using hydroxyurea (HU), followed their progression through S and G2\/M phases, and quantitatively analyzed isolated nuclear proteins by LC-MS\/MS. We identified 2,937 nuclear-enriched proteins, revealing distinct, phase-specific expression patterns. The G1\/S phase was marked by increased levels of metabolic enzymes including those related to energy and nucleotide\/nucleoside metabolisms. The S phase showed elevated abundance of canonical and variant histones, consistent with chromatin remodeling and DNA replication. The G2\/M phase was enriched in proteins involved in protein synthesis and microtubule dynamics, essential for mitosis. Notably, metabolic enzymes represented approximately 6% of the proteins identified in the purified nuclear fraction, consistent with previously published proteomic analyses of\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    nuclear-enriched fractions. Hexokinase, citrate synthase, and pyruvate kinase activities were detected in extracts from this fraction, while immunofluorescence supported the association of hexokinase and citrate synthase with the nuclear region. These results suggest that metabolic enzymes and\/or their metabolites may modulate nuclear processes in\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    , potentially influencing the epigenome and gene expression regulation, as proposed in other eukaryotic models. Altogether, our findings reveal dynamic remodeling of the nuclear proteome during the\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    cell cycle and point to a previously underappreciated role for metabolic enzymes likely regulating nuclear functions in a cell cycle-dependent manner.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013299","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:52:22Z","timestamp":1790617942000},"page":"e0013299","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nuclear proteome analysis suggests spatial and temporal compartmentalization of metabolic enzymes during Trypanosoma cruzi cell cycle"],"prefix":"10.1371","volume":"20","author":[{"given":"Ana Paula","family":"Menezes","sequence":"first","affiliation":[{"name":"Butantan Institute","place":["S\u00e3o Paulo, Brazil"],"department":["Laboratory of Cell Cycle"]},{"name":"Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Gachet-Castro","sequence":"additional","affiliation":[{"name":"Butantan Institute","place":["S\u00e3o Paulo, Brazil"],"department":["Laboratory of Cell Cycle"]},{"name":"Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"McGill","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["Glasgow, United Kingdom"],"department":["School of Infection & Immunity"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Burchmore","sequence":"additional","affiliation":[{"name":"University of Glasgow","place":["Glasgow, United Kingdom"],"department":["School of Infection & Immunity"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Silber","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"],"department":["Laboratory of Biochemistry of Tryps (LaBTryps), Department of Parasitology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carolina","family":"Elias","sequence":"additional","affiliation":[{"name":"Butantan Institute","place":["S\u00e3o Paulo, Brazil"],"department":["Laboratory of Cell Cycle"]},{"name":"Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0466-8879","authenticated-orcid":true,"given":"J\u00falia P. C.","family":"da Cunha","sequence":"additional","affiliation":[{"name":"Butantan Institute","place":["S\u00e3o Paulo, Brazil"],"department":["Laboratory of Cell Cycle"]},{"name":"Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"issue":"8","key":"pntd.0013299.ref001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008445","article-title":"Acute chagas disease in Brazil from 2001 to 2018: A nationwide spatiotemporal analysis","volume":"14","author":"EF Santos","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013299.ref002","author":"World Health Organization","year":"2022"},{"key":"pntd.0013299.ref003","unstructured":"PAHO - Pan American Health Organization. Chagas disease (American trypanosomiasis). 2022."},{"issue":"3","key":"pntd.0013299.ref004","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1053\/j.semdp.2019.04.008","article-title":"Chagas disease as example of a reemerging parasite","volume":"36","author":"J Guarner","year":"2019","journal-title":"Semin Diagn Pathol"},{"issue":"4","key":"pntd.0013299.ref005","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3109\/10409238.2013.821444","article-title":"Molecular paleontology and complexity in the last eukaryotic common ancestor","volume":"48","author":"VL Koumandou","year":"2013","journal-title":"Crit Rev Biochem Mol Biol"},{"issue":"6","key":"pntd.0013299.ref006","doi-asserted-by":"crossref","first-page":"190072","DOI":"10.1098\/rsob.190072","article-title":"Regulation of gene expression in trypanosomatids: Living with polycistronic transcription","volume":"9","author":"C Clayton","year":"2019","journal-title":"Open Biol"},{"issue":"2","key":"pntd.0013299.ref007","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0378-1119(99)00437-0","article-title":"Uridine insertion\/deletion RNA editing in trypanosome mitochondria--A review","volume":"240","author":"AM Est\u00e9vez","year":"1999","journal-title":"Gene"},{"key":"pntd.0013299.ref008","article-title":"Structure, properties, and function of glycosomes in Trypanosoma cruzi","author":"W Qui\u00f1ones","year":"2020","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"pntd.0013299.ref009","author":"RJ Wheeler","year":"2019","journal-title":"Coordination of the Cell Cycle in Trypanosomes"},{"issue":"2","key":"pntd.0013299.ref010","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.protis.2006.10.002","article-title":"Morphological events during the Trypanosoma cruzi cell cycle","volume":"158","author":"MC Elias","year":"2007","journal-title":"Protist"},{"issue":"6","key":"pntd.0013299.ref011","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1128\/EC.1.6.944-953.2002","article-title":"Chromosome localization changes in the Trypanosoma cruzi nucleus","volume":"1","author":"MCQB Elias","year":"2002","journal-title":"Eukaryot Cell"},{"issue":"2","key":"pntd.0013299.ref012","doi-asserted-by":"crossref","first-page":"136","DOI":"10.4161\/nucl.2.2.15134","article-title":"Trypanosoma cruzi DNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery","volume":"2","author":"SG Calderano","year":"2011","journal-title":"Nucleus"},{"issue":"11","key":"pntd.0013299.ref013","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.pt.2017.08.002","article-title":"Nuclear DNA replication in trypanosomatids: There are no easy methods for solving difficult problems","volume":"33","author":"MS da Silva","year":"2017","journal-title":"Trends Parasitol"},{"issue":"6","key":"pntd.0013299.ref014","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/0020-7519(94)90007-8","article-title":"The chromatin of trypanosomes","volume":"24","author":"H Hecker","year":"1994","journal-title":"Int J Parasitol"},{"key":"pntd.0013299.ref015","doi-asserted-by":"crossref","DOI":"10.1017\/erm.2012.7","article-title":"Epigenetic mechanisms, nuclear architecture and the control of gene expression in trypanosomes","volume":"14","author":"S Alsford","year":"2012","journal-title":"Expert Rev Mol Med"},{"issue":"11","key":"pntd.0013299.ref016","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0188441","article-title":"Transcriptome-wide analysis of the Trypanosoma cruzi proliferative cycle identifies the periodically expressed mRNAs and their multiple levels of control","volume":"12","author":"S Ch\u00e1vez","year":"2017","journal-title":"PLoS One"},{"issue":"5","key":"pntd.0013299.ref017","doi-asserted-by":"crossref","first-page":"140619","DOI":"10.1016\/j.bbapap.2021.140619","article-title":"Quantitative proteomics and phosphoproteomics of Trypanosoma cruzi epimastigote cell cycle","volume":"1869","author":"CMD Santos J\u00fanior A de","year":"2021","journal-title":"Biochim Biophys Acta Proteins Proteom"},{"issue":"9","key":"pntd.0013299.ref018","article-title":"Unveiling the Trypanosoma cruzi Nuclear Proteome","volume":"10","author":"CM dos Santos J\u00fanior A de","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"pntd.0013299.ref019","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1006\/excr.1994.1252","article-title":"Hydroxyurea-induced synchrony of DNA replication in the Kinetoplastida","volume":"214","author":"N Galanti","year":"1994","journal-title":"Exp Cell Res"},{"issue":"2","key":"pntd.0013299.ref020","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0166-6851(01)00430-3","article-title":"Histone H1 is phosphorylated in non-replicating and infective forms of Trypanosoma cruzi","volume":"119","author":"R Marques Porto","year":"2002","journal-title":"Mol Biochem Parasitol"},{"issue":"6","key":"pntd.0013299.ref021","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1021\/acs.jproteome.6b00208","article-title":"Chromatin proteomics reveals variable histone modifications during the life cycle of Trypanosoma cruzi","volume":"15","author":"TCL de Jesus","year":"2016","journal-title":"J Proteome Res"},{"key":"pntd.0013299.ref022","article-title":"DeepLoc 2.1: Multi-label membrane protein type prediction using protein language models","volume":"52","author":"MT \u00d8dum","year":"2024","journal-title":"Nucleic Acids Research"},{"issue":"12","key":"pntd.0013299.ref023","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005181","article-title":"Replication Protein A presents canonical functions and is also involved in the differentiation capacity of Trypanosoma cruzi","volume":"10","author":"RS Pavani","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pntd.0013299.ref024","article-title":"Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi","volume":"17","author":"ROO Souza","year":"2021","journal-title":"PLoS Pathog"},{"issue":"2","key":"pntd.0013299.ref025","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0166-6851(02)00294-3","article-title":"Molecular and biochemical characterization of hexokinase from Trypanosoma cruzi","volume":"126","author":"AJ C\u00e1ceres","year":"2003","journal-title":"Mol Biochem Parasitol"},{"issue":"13","key":"pntd.0013299.ref026","doi-asserted-by":"crossref","first-page":"3153","DOI":"10.1096\/fasebj.6.13.1397837","article-title":"Aerobic fermentation of glucose by trypanosomatids","volume":"6","author":"JJ Cazzulo","year":"1992","journal-title":"The FASEB Journal"},{"issue":"11","key":"pntd.0013299.ref027","doi-asserted-by":"crossref","first-page":"4497","DOI":"10.1021\/bi00839a041","article-title":"Escherichia coli citrate synthase. Purification and the effect of potassium on some properties","volume":"8","author":"GR Faloona","year":"1969","journal-title":"Biochemistry"},{"issue":"10","key":"pntd.0013299.ref028","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1042\/BCJ20200232","article-title":"Trypanosoma cruzi synthesizes proline via a \u03941-pyrroline-5-carboxylate reductase whose activity is fine-tuned by NADPH cytosolic pools","volume":"477","author":"L Marchese","year":"2020","journal-title":"Biochem J"},{"issue":"2","key":"pntd.0013299.ref029","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009694","article-title":"H2B.V demarcates divergent strand-switch regions, some tDNA loci, and genome compartments in Trypanosoma cruzi and affects parasite differentiation and host cell invasion","volume":"18","author":"JN Roson","year":"2022","journal-title":"PLoS Pathog"},{"key":"pntd.0013299.ref030","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.biochi.2021.10.012","article-title":"Pathogenic role of mitogen activated protein kinases in protozoan parasites","volume":"193","author":"P Kaur","year":"2022","journal-title":"Biochimie"},{"issue":"2","key":"pntd.0013299.ref031","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1006\/excr.1997.3749","article-title":"Histone synthesis inTrypanosoma cruzi","volume":"236","author":"V Sabaj","year":"1997","journal-title":"Experimental Cell Research"},{"issue":"14","key":"pntd.0013299.ref032","doi-asserted-by":"crossref","first-page":"7575","DOI":"10.1073\/pnas.1919535117","article-title":"Building blocks are synthesized on demand during the yeast cell cycle","volume":"117","author":"K Campbell","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"pntd.0013299.ref033","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0166-6851(81)90049-9","article-title":"Purine metabolism in Trypanosoma cruzi","volume":"3","author":"RL Berens","year":"1981","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"pntd.0013299.ref034","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/0014-4894(58)90019-5","article-title":"Nucleotide and polynucleotide synthesis in Trypanosoma cruzi. I. Precursors of purine compounds","volume":"7","author":"JF Fernandes","year":"1958","journal-title":"Exp Parasitol"},{"issue":"4","key":"pntd.0013299.ref035","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/0092-8674(94)90543-6","article-title":"Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age","volume":"79","author":"T Hunter","year":"1994","journal-title":"Cell"},{"issue":"3","key":"pntd.0013299.ref036","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/j.1365-2958.2008.06443.x","article-title":"The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: A role for translational control in cytokinesis","volume":"70","author":"S Regmi","year":"2008","journal-title":"Mol Microbiol"},{"key":"pntd.0013299.ref037","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.copbio.2014.11.022","article-title":"Metabolic pathway compartmentalization: An underappreciated opportunity?","volume":"34","author":"A Zecchin","year":"2015","journal-title":"Curr Opin Biotechnol"},{"issue":"1","key":"pntd.0013299.ref038","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.tibs.2017.11.004","article-title":"Spatiotemporal control of acetyl-CoA metabolism in chromatin regulation","volume":"43","author":"S Sivanand","year":"2018","journal-title":"Trends Biochem Sci"},{"issue":"2","key":"pntd.0013299.ref039","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcel.2017.06.008","article-title":"Nuclear acetyl-CoA production by ACLY promotes homologous recombination","volume":"67","author":"S Sivanand","year":"2017","journal-title":"Molecular Cell"},{"issue":"3","key":"pntd.0013299.ref040","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/s41564-022-01295-6","article-title":"Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei","volume":"8","author":"K Billington","year":"2023","journal-title":"Nat Microbiol"},{"key":"pntd.0013299.ref041","article-title":"The nuclear pore complex and nuclear transport","author":"SR Wente","year":"2010","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"issue":"21","key":"pntd.0013299.ref042","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1080\/15384101.2015.1090068","article-title":"Metabolic control of the cell cycle","volume":"14","author":"J Kalucka","year":"2015","journal-title":"Cell Cycle"},{"key":"pntd.0013299.ref043","doi-asserted-by":"crossref","first-page":"100415","DOI":"10.1016\/j.coisb.2022.100415","article-title":"Metabolic dynamics during the cell cycle","volume":"30","author":"A Zylstra","year":"2022","journal-title":"Curr Opin Syst Biol"},{"issue":"9","key":"pntd.0013299.ref044","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1101\/gad.1790409","article-title":"Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei","volume":"23","author":"TN Siegel","year":"2009","journal-title":"Genes Dev"},{"key":"pntd.0013299.ref045","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1177\/1947601912473308","article-title":"The CDK network: Linking cycles of cell division and gene expression","volume":"3","author":"RP Fisher","year":"2012","journal-title":"Genes Cancer"},{"issue":"1","key":"pntd.0013299.ref046","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.cell.2014.04.046","article-title":"A nuclear pyruvate dehydrogenase complex is important for the generation of acetyl-CoA and histone acetylation","volume":"158","author":"G Sutendra","year":"2014","journal-title":"Cell"},{"issue":"3","key":"pntd.0013299.ref047","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0120896","article-title":"Glyceraldehyde 3-phosphate dehydrogenase-telomere association correlates with redox status in Trypanosoma cruzi","volume":"10","author":"R Pariona-Llanos","year":"2015","journal-title":"PLoS One"},{"key":"pntd.0013299.ref048","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2016.12.026","article-title":"Nuclear localization of mitochondrial TCA cycle enzymes as a critical step in mammalian zygotic genome activation","volume":"168","author":"R Nagaraj","year":"2017","journal-title":"Cell"},{"key":"pntd.0013299.ref049","doi-asserted-by":"crossref","first-page":"105413","DOI":"10.1016\/j.jprot.2025.105413","article-title":"Trypanosoma cruzi cell cycle progression exhibits minimal variation in histone PTMs with unique histone H4 acetylation pattern","volume":"315","author":"APJ Menezes","year":"2025","journal-title":"J Proteomics"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0013299","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:00:07Z","timestamp":1791396007000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013299"}},"subtitle":[],"editor":[{"given":"Claudia Ida","family":"Brodskyn","sequence":"first","affiliation":[{"name":"Centro de Pesquisa Gon\u00e7alo Moniz-FIOCRUZ\/BA","place":["BRAZIL"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,28]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0013299","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,28]]}}}