{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:35:27Z","timestamp":1789061727327,"version":"build-2803163510"},"reference-count":115,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB-2223973"],"award-info":[{"award-number":["DEB-2223973"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Washington College of the Environment"},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-214-0004"],"award-info":[{"award-number":["DGE-214-0004"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB-2141898"],"award-info":[{"award-number":["DEB-2141898"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Echinococcus multilocularis<\/jats:italic>\n                    is a zoonotic cestode that uses canids as definitive hosts and rodents as intermediate hosts. In humans, this parasite is the causative agent of alveolar echinococcosis. Recently, its range has been expanding across the Northern Hemisphere, and it is increasingly detected in wild canids, domestic dogs, and humans across Canada and the United States. While this expansion has been documented in isolated studies across the continent, a lack of routine sampling in wildlife hinders our ability to anticipate and mitigate further spread of\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    . We confirmed the presence of\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    in Washington State, USA, using a combination of morphological and molecular techniques across carcasses and field-collected scats of coyotes (\n                    <jats:italic>Canis latrans<\/jats:italic>\n                    ), this region\u2019s most common wild canid. Morphological identification of adult worms was confirmed by next-generation sequencing. Over a third of all samples tested positive for\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    when all methodologies were combined. Sequencing revealed a haplotype of\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    matching a documented haplotype originally of European origin in British Columbia, Canada. Our study provides the first confirmation of\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    in a wild host on the west coast of the contiguous United States and provides additional haplotype information crucial to tracking the geographical expansion of the parasite. We also provide a new next-generation sequencing primer targeting cestodes of canids. The difference in amplification between intestinal and fecal samples suggests that non-invasive fecal sampling using DNA metabarcoding - a popular method of helminth surveillance - may lead to underestimation of prevalence, hindering control measures. The global significance of these findings extends beyond North America;\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    is a major public health concern in Europe and Asia, where alveolar echinococcosis is increasingly diagnosed in humans. Our study highlights the urgent need for increased surveillance and improved diagnostic strategies worldwide, particularly in regions with significant human-wildlife contact.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013502","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T17:42:13Z","timestamp":1774374133000},"page":"e0013502","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Echinococcus multilocularis in coyotes in Washington State, USA highlights need for increased wildlife surveillance"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3531-6956","authenticated-orcid":true,"given":"Yasmine","family":"Hentati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Reese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire C.","family":"Curran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika M.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dakeishla M.","family":"D\u00edaz-Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha E.S.","family":"Kreling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme G.","family":"Verocai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura R.","family":"Prugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Schell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea L.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"key":"pntd.0013502.ref001","doi-asserted-by":"crossref","unstructured":"Massolo A, Liccioli S, Budke C, Klein C. 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