{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:35:11Z","timestamp":1792118111193,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013533","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000}}],"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP19680079"],"award-info":[{"award-number":["AP19680079"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>The genetic diversity and biovar classification of <jats:italic><jats:italic>Yersinia<\/jats:italic><\/jats:italic> isolates from Central Asia were investigated using whole-genome sequencing. In total, 98 isolates from natural plague foci were sequenced using the MiSeq platform. Computational pipelines were developed for accurate assembly of <jats:italic><jats:italic>Y. pestis<\/jats:italic><\/jats:italic> replicons, including small cryptic plasmids, and for identifying genetic polymorphisms. A panel of 99 diagnostic polymorphisms was established, enabling the distinction of dominant Medievalis isolates derived from desert and upland regions. Evidence of convergent evolution was observed in polymorphic allele distributions across genetically distinct <jats:italic><jats:italic>Y. pestis<\/jats:italic><\/jats:italic> biovars, <jats:italic><jats:italic>Y. pseudotuberculosis<\/jats:italic><\/jats:italic>, and other <jats:italic><jats:italic>Y. pestis<\/jats:italic><\/jats:italic> strains, likely driven by adaptation to similar environmental conditions. Genetic polymorphisms in the <jats:italic><jats:italic>napA<\/jats:italic><\/jats:italic>, <jats:italic><jats:italic>araC<\/jats:italic><\/jats:italic>, <jats:italic><jats:italic>ssuA<\/jats:italic><\/jats:italic>, and <jats:italic><jats:italic>rhaS<\/jats:italic><\/jats:italic> genes, along with transposon and CRISPR-Cas insertion patterns, were confirmed as suitable tools for identifying <jats:italic><jats:italic>Y. pestis<\/jats:italic><\/jats:italic> biovars, although their homoplasy suggests limited utility for phylogenetic inference. Notably, a novel cryptic plasmid, pCKF, previously associated with the strain of the population 2.MED0 from the Central-Caucasus high-altitude autonomous plague focus, was detected in a genetically distinct isolate of 2.MED1 population from the Ural-Embi region, indicating potential plasmid transfer across the 2.MED lineage. These findings emphasize the need for ongoing genomic surveillance to monitor the spread of virulence-associated genetic elements and to improve our understanding of <jats:italic><jats:italic>Y. pestis<\/jats:italic><\/jats:italic> evolution and ecology.<\/jats:p>","DOI":"10.1371\/journal.pntd.0013533","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:57:32Z","timestamp":1757699852000},"page":"e0013533","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Whole genome sequencing of Yersinia pestis isolates from Central Asian natural plague foci revealed the role of adaptation to different hosts and environmental conditions in shaping specific genotypes"],"prefix":"10.1371","volume":"19","author":[{"given":"Aigul A.","family":"Abdirassilova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duman T.","family":"Yessimseit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Altynai K.","family":"Kassenova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beck Z.","family":"Abdeliyev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zauresh B.","family":"Zhumadilova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulnara Zh.","family":"Tokmurziyeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina G.","family":"Kovaleva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyat Zh.","family":"Abdel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiyana V.","family":"Meka-Mechenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saule K.","family":"Umarova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmira Zh.","family":"Begimbayeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanzhar D.","family":"Agzam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir L.","family":"Motin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5459-2772","authenticated-orcid":true,"given":"Oleg N.","family":"Reva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8684-3425","authenticated-orcid":true,"given":"Altyn K.","family":"Rysbekova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"issue":"1","key":"pntd.0013533.ref001","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.10.1.35","article-title":"Yersinia pestis--etiologic agent of plague","volume":"10","author":"RD Perry","year":"1997","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"pntd.0013533.ref002","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00044-19","article-title":"Yersinia pestis: the Natural History of Plague","volume":"34","author":"R Barbieri","year":"2020","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"pntd.0013533.ref003","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.amjmed.2020.08.019","article-title":"History of the Plague: An Ancient Pandemic for the Age of COVID-19","volume":"134","author":"KA Glatter","year":"2021","journal-title":"Am J Med"},{"issue":"1","key":"pntd.0013533.ref004","doi-asserted-by":"crossref","first-page":"146","DOI":"10.3390\/microorganisms12010146","article-title":"The Natural and Clinical History of Plague: From the Ancient Pandemics to Modern Insights","volume":"12","author":"A Bennasar-Figueras","year":"2024","journal-title":"Microorganisms"},{"issue":"2","key":"pntd.0013533.ref005","first-page":"247","article-title":"Varieties of Pasteurella pestis; 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