{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:58:37Z","timestamp":1791003517280,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013541","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000}}],"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008897","name":"Janssen Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001713","name":"European and Developing Countries Clinical Trials Partnership","doi-asserted-by":"publisher","award":["RIA2017NIM-1847-PEOPLE"],"award-info":[{"award-number":["RIA2017NIM-1847-PEOPLE"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Research Foundation Flanders","doi-asserted-by":"crossref","award":["1SE7522N + 1SE7524N"],"award-info":[{"award-number":["1SE7522N + 1SE7524N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100016059","name":"Action Damien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016059","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>The accuracy of the WHO-endorsed clinical leprosy diagnosis depends on the expertise of health care workers. For molecular confirmation of clinically diagnosed patients, skin biopsies have the highest sensitivity to detect <jats:italic><jats:italic>Mycobacterium leprae<\/jats:italic><\/jats:italic>. As less invasive tongue swabs showed promising results for qPCR-based <jats:italic><jats:italic>M. tuberculosis<\/jats:italic><\/jats:italic> detection, this study investigated the presence of <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> on the tongue dorsum of clinically diagnosed leprosy patients.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods and findings<\/jats:title>\n<jats:p>During the activities of the (BE-)PEOPLE study, 499 clinically diagnosed, consenting patients from the Comoros were recruited. Samples collected included skin biopsies from active lesions, nasal swabs, tongue swabs, and, in some cases, tongue scrapes. <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> DNA was quantified with the RLEP qPCR assay and human mitochondrial DNA was quantified as sample adequacy control (SAC).<\/jats:p>\n<jats:p>On 18.1% (90\/498) of tongue swabs and 13.2% (12\/91) of tongue scrapes <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> DNA was detected. In only six patients tongue scrapes outperformed the tongue swab based on the number of bacilli\/sample. Except for two paucibacillary (PB) patients, all 100\/102 positive tongue samples were from multibacillary (MB) patients. Only patients with a RLEP-positive skin biopsy and positive bacteriological index (BI) yielded <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> DNA on the tongue scrape. The skin biopsy samples had a sensitivity of 92.5% (248\/268) for MB and 74.3% (130\/175) for paucibacillary (PB) patients. Nasal swabs were positive for 60.2% (162\/269) of MB but only 2.2% (5\/229) of PB patients.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>This is the first study to identify <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> bacilli on the tongue dorsum of clinically diagnosed leprosy patients by RLEP qPCR. Due to low positivity rates, tongue sampling has limited added value over skin biopsies and nasal swabs for the microbiological confirmation of leprosy. However, the mouth in general and the tongue specifically remain interesting sampling sites to gain further insights on the distribution of <jats:italic><jats:italic>M. leprae<\/jats:italic><\/jats:italic> bacilli in the body and potential transmission modes.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0013541","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T17:42:36Z","timestamp":1758130956000},"page":"e0013541","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploration of tongue dorsum sampling to support clinical diagnosis of leprosy patients in the Comoros: A cross-sectional study"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4077-8664","authenticated-orcid":true,"given":"Lena","family":"Krausser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maren","family":"Van Nieuwenhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nissad","family":"Attoumani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silahi H.","family":"Grillone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magalie","family":"Van Dyck-Lippens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen","family":"Rigouts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdallah","family":"Baco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wirdane","family":"Abdou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aboubacar","family":"Mzembaba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Epco","family":"Hasker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Younoussa","family":"Assoumani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bouke C.","family":"de Jong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie M.","family":"Braet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,17]]},"reference":[{"key":"pntd.0013541.ref001","doi-asserted-by":"crossref","unstructured":"Deps P, Collin SM. 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