{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:57:08Z","timestamp":1787021828179,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013640","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000}}],"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["MOST 106-2314-B-075B-009 -MY3; MOST 109-2314-B-075B-013 -MY3"],"award-info":[{"award-number":["MOST 106-2314-B-075B-009 -MY3; MOST 109-2314-B-075B-013 -MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011913","name":"Kaohsiung Veterans General Hospital","doi-asserted-by":"publisher","award":["KSVGH110-059; 107-079; 111-087"],"award-info":[{"award-number":["KSVGH110-059; 107-079; 111-087"]}],"id":[{"id":"10.13039\/501100011913","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    is a soil-borne pathogen responsible for melioidosis, a potentially fatal disease. While endemic melioidosis in Taiwan is marked by both clinical cases and environmental detection, a comprehensive environmental survey has been lacking. A nationwide cropland survey using\n                    <jats:italic>B. pseudomallei-<\/jats:italic>\n                    specific\n                    <jats:italic>orf2-<\/jats:italic>\n                    PCR revealed regional positivity rates of 2.1% in northern, 8.2% in central, 15.1% in southern, and 9.8% in eastern Taiwan, with the highest PCR positivity and cumulative melioidosis incidence (12.14 cases per 100,000 people from 2003 to 2024) observed in the south. Vertical profiling showed a higher DNA detection rate at a depth of 60\u2009cm, with increased surface-level detection during rainy periods and a decline after sunny conditions, particularly in the southern area. Identical molecular sequence types across layers suggested upward movement from deeper soil. However, viable bacteria were not consistently recovered from PCR-positive samples, indicating that bacterial dynamics during incubation may influence\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    viability. To investigate this, full-length 16S rDNA sequencing and community analysis revealed inverse abundance patterns between\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    and\n                    <jats:italic>B. multivorans<\/jats:italic>\n                    ,\n                    <jats:italic>B. cenocepacia<\/jats:italic>\n                    , and B.\n                    <jats:italic>vietnamiensis<\/jats:italic>\n                    during incubation. In vitro assays confirmed strong antagonism by\n                    <jats:italic>B. multivorans<\/jats:italic>\n                    filtered supernatants against the growth of\n                    <jats:italic>B. pseudomallei,<\/jats:italic>\n                    while\n                    <jats:italic>B. cenocepacia<\/jats:italic>\n                    and\n                    <jats:italic>B. vietnamiensis<\/jats:italic>\n                    had weaker or no effects. These findings reveal distinct regional and vertical patterns of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    DNA in croplands and highlight the potential influence of bacterial competition on its viability during incubation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013640","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:47:59Z","timestamp":1761155279000},"page":"e0013640","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Geographical distribution of Burkholderia pseudomallei in Taiwanese croplands and the influence of bacterial community interactions on its incubation 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