{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:52:17Z","timestamp":1792788737527,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013754","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000}}],"reference-count":79,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AgriSETA","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"DOI":"10.13039\/100018370","name":"Instituut voor Tropische Geneeskunde","doi-asserted-by":"publisher","award":["FA4 DGD-ITM 2017-2021 & FA5 DGD-ITM 2022-2026"],"award-info":[{"award-number":["FA4 DGD-ITM 2017-2021 & FA5 DGD-ITM 2022-2026"]}],"id":[{"id":"10.13039\/100018370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Brucellosis is a globally significant zoonotic disease, yet its ecology in wildlife remains poorly understood. In this study, we investigated the circulation of\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. in a wide range of wild mammals from multiple protected areas in South Africa. Organ and serum samples from 722 animals representing 23 species were analyzed employing a conservative diagnostic strategy, combining serology (rose bengal test confirmed by indirect ELISA) and four PCR-based assays in series, to maximize specificity and interpretative solidity. Molecular detection revealed\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. DNA in several atypical host species, including plains zebra, hippopotamus, African elephant, giraffe, warthog, cheetah, and African wild dog, expanding the known host range. In Greater Kruger National Park, African buffalo exhibited high seroprevalence (24\/106; 23%, CI: 15\u201332%) and even higher molecular prevalence (29\/57; 51%, CI: 37\u201364%), supporting their role as a primary wildlife reservoir for\n                    <jats:italic>B. abortus<\/jats:italic>\n                    in the region. One\n                    <jats:italic>B. abortus<\/jats:italic>\n                    isolate cultured from the spleen of a zebra and characterized by multiloci variable number of tandem repeat analysis (MLVA) showed genetic relatedness to South African buffalo and South American cattle strains. Co-infections with\n                    <jats:italic>B. abortus<\/jats:italic>\n                    and\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    were identified in 17 animals across seven species. Notably,\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    was detected in species (elephant, hippopotamus, zebra) not typically associated with small ruminants, suggesting complex interspecies transmission dynamics. Our findings underscore the limitations of serological testing and highligts the value of molecular diagnostics in understanding the epidemiology of\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. in South Africa. The detection of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>abortu<\/jats:italic>\n                    s and\/or\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    DNA in a range of wildlife species, including carnivores and megaherbivores, emphasises the need for integrated One Health surveillance approaches to enhance understanding of the disease\u2019s life cycle and transmission dynamics.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013754","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T18:43:13Z","timestamp":1765478593000},"page":"e0013754","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Epidemiological baseline of Brucella spp. in South African wildlife"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3334-8498","authenticated-orcid":true,"given":"Carlo Andrea","family":"Cossu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette","family":"Wentzel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Mari","family":"de Klerk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"De Massis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Godfroid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis Ockert","family":"van Schalkwyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliano","family":"Garofolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henriette","family":"van Heerden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"issue":"9","key":"pntd.0013754.ref001","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.3201\/eid2909.230052","article-title":"Global Estimate of Human Brucellosis Incidence","volume":"29","author":"CG Laine","year":"2023","journal-title":"Emerg Infect Dis"},{"key":"pntd.0013754.ref002","doi-asserted-by":"crossref","DOI":"10.1016\/S0368-1742(97)80014-8","article-title":"The Etiology of Epizootic Abortion","volume":"10","author":"B Bang","year":"1897","journal-title":"Journal of Comparative Pathology and Therapeutics"},{"issue":"6","key":"pntd.0013754.ref003","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1093\/infdis\/22.6.580","article-title":"Further Studies on Bacterium Abortus and Related Bacteria: II. A Comparison of Bacterium Abortus with Bacterium Bronchisepticus and with the Organism which Causes Malta Fever","volume":"22","author":"AC Evans","year":"1918","journal-title":"Journal of Infectious Diseases"},{"issue":"3","key":"pntd.0013754.ref004","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1093\/infdis\/27.3.173","article-title":"A Comparison of the Morphologic, Cultural and Biochemical Characteristics of B. Abortus and B. Melitensis: Studies on the Genus Brucella Nov. Gen. 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Moreno","year":"2014","journal-title":"Front Microbiol"},{"key":"pntd.0013754.ref018","first-page":"1510","article-title":"Bovine brucellosis","volume-title":"Infectious Diseases of Livestock","author":"J Godfroid","year":"2004"},{"key":"pntd.0013754.ref019","first-page":"1","article-title":"Brucellosis","volume-title":"Terrestrial manual","author":"WOAH","year":"2022"},{"issue":"12","key":"pntd.0013754.ref020","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.3201\/eid1612.100678","article-title":"Brucellosis reactivation after 28 years","volume":"16","author":"\u00d6 \u00d6gredici","year":"2010","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"pntd.0013754.ref021","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010404","article-title":"Human brucellosis: Widespread information deficiency hinders an understanding of global disease frequency","volume":"16","author":"CG Laine","year":"2022","journal-title":"PLoS Negl Trop 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The effects of land-use change on brucellosis transmission in the greater yellowstone ecosystem: A three species mathematical-epidemiological model. 2022. doi: 10.48550\/arXiv.2208.01039"},{"issue":"4","key":"pntd.0013754.ref072","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0147-9571(93)90159-3","article-title":"Reactions of sera from laboratory, domestic and wild animals in Africa with protein A and a recombinant chimeric protein AG","volume":"16","author":"PJ Kelly","year":"1993","journal-title":"Comp Immunol Microbiol Infect Dis"},{"issue":"3","key":"pntd.0013754.ref073","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1177\/1040638713485073","article-title":"A protein A\/G indirect enzyme-linked immunosorbent assay for the detection of anti-Brucella antibodies in Arctic wildlife","volume":"25","author":"IH Nymo","year":"2013","journal-title":"J Vet Diagn Invest"},{"key":"pntd.0013754.ref074","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1438-4221(02)80018-2","article-title":"A new non-species dependent ELISA for detection of antibodies to Borrelia burgdorferi s. l. in zoo animals","author":"K St\u00f6bel","year":"2002","journal-title":"Int J Med Microbiol"},{"key":"pntd.0013754.ref075","doi-asserted-by":"crossref","first-page":"1221071","DOI":"10.3389\/fimmu.2023.1221071","article-title":"Development of conjugated secondary antibodies for wildlife disease surveillance","volume":"14","author":"SO Ochai","year":"2023","journal-title":"Front Immunol"},{"issue":"4","key":"pntd.0013754.ref076","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"J Godfroid","year":"2010","journal-title":"Croat Med J"},{"key":"pntd.0013754.ref077","first-page":"445","article-title":"Impediments to wildlife disease surveillance, research, and diagnostics","volume":"315","author":"DE Stallknecht","year":"2007","journal-title":"Curr Top Microbiol Immunol"},{"issue":"1","key":"pntd.0013754.ref078","doi-asserted-by":"crossref","first-page":"76","DOI":"10.3957\/056.045.0109","article-title":"Break on Through to the Other Side: The Effectiveness of Game Fencing to Mitigate Human\u2014Wildlife Conflict","volume":"45","author":"MK Kesch","year":"2015","journal-title":"African Journal of Wildlife Research"},{"issue":"1","key":"pntd.0013754.ref079","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0168-1702(02)00263-0","article-title":"Foot and mouth disease in wildlife","volume":"91","author":"GR Thomson","year":"2003","journal-title":"Virus Res"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0013754","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013754","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T18:46:30Z","timestamp":1766169990000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013754"}},"subtitle":[],"editor":[{"given":"Stuart D.","family":"Blacksell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,12,11]]},"references-count":79,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0013754","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,11]]}}}