{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:20:17Z","timestamp":1767774017356,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013848","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000}}],"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:00:00Z","timestamp":1766707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006209","name":"Radboud University Medical Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006209","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Malaria remains a leading cause of morbidity and mortality in large parts of the world. Resistance threatens current preventative and curative strategies, highlighting the need for novel complementary strategies. The opportunities that the mosquito virome may offer to reduce pathogen transmission have not been systematically explored for malaria control. In this study, we assessed whether insect-specific viruses affect\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    development in mosquitoes. A panel of 15 viruses was tested for\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    replication in anopheline cells and mosquitoes. From this panel, the RNA viruses Flock House virus, Wallerfield virus, Agua Salud alphavirus, Herbert herbevirus and Goul\u00e9ako goukovirus and the dsDNA virus invertebrate iridescent virus 6 efficiently replicated in two\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    cell lines and were further assessed in\n                    <jats:italic>in vivo<\/jats:italic>\n                    experiments. Intrathoracic injection of these viruses in\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    mosquitoes resulted in efficient viral RNA replication for Herbert herbevirus, Agua Salud alphavirus and Goul\u00e9ako goukovirus and high levels of infectious viral particle production for invertebrate iridescent virus 6. In contrast, Wallerfield virus showed poor replication, whereas Flock House virus replicated efficiently but caused high mosquito mortality. Subsequently, we performed\n                    <jats:italic>in vivo<\/jats:italic>\n                    coinfections of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    with the four efficiently replicating insect-specific viruses that were not associated with high mosquito mortality. Sporozoite formation and\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    infection rates did not differ between virus-infected mosquitoes and non-infected controls. While none of the tested viruses significantly affected\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    development, our study identified multiple viruses that efficiently infect\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    mosquitoes, providing a useful resource to study\n                    <jats:italic>Anopheles<\/jats:italic>\n                    immunity. Future studies may address whether native\n                    <jats:italic>Anopheles<\/jats:italic>\n                    -specific viruses affect\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    parasite transmission.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013848","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T18:46:51Z","timestamp":1766774811000},"page":"e0013848","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Systemic infection with insect-specific viruses does not affect Plasmodium sporozoite formation in Anopheles mosquitoes"],"prefix":"10.1371","volume":"19","author":[{"given":"Michelle","family":"Schinkel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gijs 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