{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T03:25:11Z","timestamp":1783049111062,"version":"3.54.6"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013871","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000}}],"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016399","name":"University of Reims Champagne-Ardenne","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100016399","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Nitisinone, a systemic inhibitor of tyrosine catabolism, has recently emerged as a promising endectocide with demonstrated lethality against mosquitoes and tsetse flies. To assess its efficacy against\n                    <jats:italic>Lutzomyia longipalpis<\/jats:italic>\n                    , an important vector of\n                    <jats:italic>Leishmania infantum<\/jats:italic>\n                    \u2014the causative agent of zoonotic visceral leishmaniasis prevalent in the Mediterranean basin, Latin America, and parts of the Middle East\u2014, we exposed sand flies to a concentration previously shown to be lethal to\n                    <jats:italic>Anopheles<\/jats:italic>\n                    mosquitoes (250 ng\/mL). Survival was monitored over 14 days and showed no significant difference between treated and control groups, indicating a lack of insecticidal effect at this dose. These findings suggest that\n                    <jats:italic>Lu. longipalpis<\/jats:italic>\n                    exhibits tolerance to nitisinone, likely due to species-specific metabolic differences. Although nitisinone remains a promising tool for systemic vector control, its effectiveness against sand flies may require higher doses or alternative compounds.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013871","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T18:44:05Z","timestamp":1768243445000},"page":"e0013871","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluating the impact of nitisinone at mosquito-lethal doses on Lutzomyia longipalpis"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6271-1508","authenticated-orcid":true,"given":"Laure","family":"Augendre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Alexandre Farias de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda Clara Vieira da","family":"Costa Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Depaquit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Martinet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorian","family":"Prudhomme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"issue":"1","key":"pntd.0013871.ref001","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/s42003-022-03240-z","article-title":"Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites","volume":"5","author":"P Cec\u00edlio","year":"2022","journal-title":"Commun Biol"},{"issue":"1","key":"pntd.0013871.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2915.2003.00420.x","article-title":"Control of phlebotomine sandflies","volume":"17","author":"B Alexander","year":"2003","journal-title":"Med Vet Entomol"},{"issue":"8","key":"pntd.0013871.ref003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009586","article-title":"Chemical control and insecticide resistance status of sand fly vectors worldwide","volume":"15","author":"S Balaska","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0013871.ref004","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007831","article-title":"The importance of vector control for the control and elimination of vector-borne diseases","volume":"14","author":"AL Wilson","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0013871.ref005","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.jcpa.2024.01.001","article-title":"Sand fly-borne diseases in Europe: epidemiological overview and potential triggers for their emergence and re-emergence","volume":"209","author":"C Maia","year":"2024","journal-title":"J Comp Pathol"},{"issue":"2","key":"pntd.0013871.ref006","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1046\/j.1365-2915.2000.00237.x","article-title":"Sampling methods for phlebotomine sandflies","volume":"14","author":"B Alexander","year":"2000","journal-title":"Med Vet Entomol"},{"issue":"2","key":"pntd.0013871.ref007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009062","article-title":"Exploring the influence of different habitats and their volatile chemistry in modulating sand fly population structure in a leishmaniasis endemic foci, Kenya","volume":"15","author":"IB Hassaballa","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"issue":"10","key":"pntd.0013871.ref008","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.pt.2011.05.007","article-title":"Endectocides for malaria control","volume":"27","author":"BD Foy","year":"2011","journal-title":"Trends Parasitol"},{"issue":"1","key":"pntd.0013871.ref009","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1365-2915.2012.00995.x","article-title":"Feed-through insecticides for the control of the sand fly Phlebotomus argentipes","volume":"27","author":"K Ingenloff","year":"2013","journal-title":"Med Vet Entomol"},{"issue":"5","key":"pntd.0013871.ref010","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/ja.2017.11","article-title":"Ivermectin: enigmatic multifaceted \u201cwonder\u201d drug continues to surprise and exceed expectations","volume":"70","author":"A Crump","year":"2017","journal-title":"J Antibiot (Tokyo)"},{"issue":"1","key":"pntd.0013871.ref011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.3000796","article-title":"Repurposing the orphan drug nitisinone to control the transmission of African trypanosomiasis","volume":"19","author":"M Sterkel","year":"2021","journal-title":"PLoS Biol"},{"key":"pntd.0013871.ref012","doi-asserted-by":"crossref","unstructured":"McComic SE, Duke SO, Burgess ER 4th, Swale DR. Defining the toxicological profile of 4-hydroxyphenylpyruvate dioxygenase-directed herbicides to Aedes aegypti and Amblyomma americanum. Pestic Biochem Physiol. 2023;194:105532. doi: 10.1016\/j.pestbp.2023.105532 37532340","DOI":"10.1016\/j.pestbp.2023.105532"},{"issue":"16","key":"pntd.0013871.ref013","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1016\/j.cub.2016.06.025","article-title":"Tyrosine Detoxification Is an Essential Trait in the Life History of Blood-Feeding Arthropods","volume":"26","author":"M Sterkel","year":"2016","journal-title":"Curr Biol"},{"issue":"16","key":"pntd.0013871.ref014","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2016.06.068","article-title":"Vector Biology: Tyrosine Degradation Protects Blood Feeders from Death via La Grande Bouffe","volume":"26","author":"P Kop\u00e1\u010dek","year":"2016","journal-title":"Curr Biol"},{"key":"pntd.0013871.ref015","unstructured":"Prudhomme J, Laroche L, Qu\u00e2n ANH, Fournier J, Mhaidi I, Berhard A, et al. Establishment and maintenance of two sand fly colonies (Phlebotomus perniciosus and Phlebotomus papatasi) to implement experimental infections [Internet]. 2023 [cited 2025 Jun 3]. Available from: http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2023.07.19.549665"},{"issue":"791","key":"pntd.0013871.ref016","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.adr4827","article-title":"Anopheles mosquito survival and pharmacokinetic modeling show the mosquitocidal activity of nitisinone","volume":"17","author":"LR Haines","year":"2025","journal-title":"Sci Transl Med"},{"issue":"2","key":"pntd.0013871.ref017","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/jme\/tjz199","article-title":"Comparative Study of Promastigote- and Amastigote-Initiated Infection of Leishmania infantum (Kinetoplastida: Trypanosomatidae) in Phlebotomus perniciosus (Diptera: Psychodidae) Conducted in Different Biosafety Level Laboratories","volume":"57","author":"S Vaselek","year":"2020","journal-title":"J Med Entomol"},{"key":"pntd.0013871.ref018","unstructured":"Therneau T. A Package for survival analysis in R. R package version 3.6-4;2024; https:\/\/CRAN.R-project.org\/package=survival.therneau"},{"key":"pntd.0013871.ref019","doi-asserted-by":"crossref","unstructured":"Therneau TM, Grambsch PM. Modeling Survival Data: Extending the Cox Model [Internet]. New York, NY: Springer New York; 2000 [cited 2025 Jun 3]. (Dietz K, Gail M, Krickeberg K, Samet J, Tsiatis A, editors. Statistics for Biology and Health). Available from: http:\/\/link.springer.com\/10.1007\/978-1-4757-3294-8","DOI":"10.1007\/978-1-4757-3294-8"},{"key":"pntd.0013871.ref020","doi-asserted-by":"crossref","unstructured":"Kassambara A, Kosinski M, Biecek P. survminer: Drawing Survival Curves using \u2018ggplot2\u2019 [Internet]. 2016 [cited 2025 Jun 3]. p. 0.5.0. Available from: https:\/\/CRAN.R-project.org\/package=survminer","DOI":"10.32614\/CRAN.package.survminer"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0013871","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013871","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T18:43:44Z","timestamp":1769021024000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0013871"}},"subtitle":[],"editor":[{"given":"Michael","family":"Marks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,12]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0013871","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,12]]}}}