{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T21:00:01Z","timestamp":1769634001230,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0013915","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000}}],"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802003"],"award-info":[{"award-number":["81802003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018628","name":"Scientific Research Foundation of Education Department of Anhui Province of China","doi-asserted-by":"publisher","award":["2022AH050751"],"award-info":[{"award-number":["2022AH050751"]}],"id":[{"id":"10.13039\/501100018628","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Anhui Medical University Doctoral Scientific Research Foundation","award":["xj201725"],"award-info":[{"award-number":["xj201725"]}]},{"name":"National Key Laboratory of Pathogen Microbiology Biosafety Open Fund Project","award":["SKLPBS2456"],"award-info":[{"award-number":["SKLPBS2456"]}]},{"name":"Anhui Provincial Key Laboratory of Pathogen Biology Open Fund Project","award":["BY-2022Z04"],"award-info":[{"award-number":["BY-2022Z04"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    , a widespread intracellular protozoan parasite, infects a significant portion of the global human population. Restricted to an acute-infection model, this study elucidates the role of the host protein PIM1, a serine\/threonine protein kinase, in facilitating\n                    <jats:italic>T. gondii<\/jats:italic>\n                    proliferation and its potential as a therapeutic target. Employing both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    models, we establish that PIM1 enhances the intracellular proliferation of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    by suppressing host cell apoptosis. Our findings underscore the necessity of PIM1\u2019s kinase activity in this process, as evidenced by the significant reduction in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    proliferation upon treatment with either a kinase-dead PIM1 mutant or the PIM1 inhibitor AZD1208. In murine models, AZD1208 treatment resulted in decreased\n                    <jats:italic>T. gondii<\/jats:italic>\n                    load and elevated pro-apoptotic markers in tissues, indicating that PIM1 inhibition bolsters the host\u2019s immune response against the parasite. Since the role of PIM1 in chronic infection remains unexplored, follow-up studies using chronic models are essential. Collectively, our findings illuminate host\u2013parasite interplay during acute toxoplasmosis and position PIM1 as a promising target for anti-\n                    <jats:italic>T. gondii<\/jats:italic>\n                    therapeutics.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0013915","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T18:50:27Z","timestamp":1768935027000},"page":"e0013915","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling the role of host kinase PIM1 in Toxoplasma gondii infection: Implications for 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