{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:51:29Z","timestamp":1776833489188,"version":"3.51.2"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014065","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000}}],"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:00:00Z","timestamp":1772755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2515340"],"award-info":[{"award-number":["2515340"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["NIH K01OD037645"],"award-info":[{"award-number":["NIH K01OD037645"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The emergence of flaviviruses and other arboviruses in novel geographic locations, arthropod vectors, and vertebrate amplification hosts complicates control and eradication efforts. Many flaviviruses continue to impact global health, including Zika virus (ZIKV) which has expanded to a global health threat, yet many questions remain surrounding its ecology and inter-epidemic maintenance. While bats are known to harbor several medically important viruses without clinical disease, their role in the sylvatic transmission cycle of arboviruses remains unresolved. Reports describing the susceptibility of different bat species to infection with ZIKV are inconsistent, and the immunological mechanisms underpinning this variability have not been well-characterized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal Findings<\/jats:title>\n                    <jats:p>\n                      We compared the permissiveness and immune responses of cell lines derived from two frugivorous bat species: the Egyptian fruit bat (\n                      <jats:italic>Rousettus aegyptiacus<\/jats:italic>\n                      ) and Jamaican fruit bat (\n                      <jats:italic>Artibeus jamaicensis<\/jats:italic>\n                      ). Using multi-step growth curves, we compared infection dynamics of two genetically distinct ZIKV strains, African lineage (ZIKV\/MR766) and Asian-American lineage (ZIKV\/PRVABC59) and compared transcriptomic responses to both lineages in susceptible bat-derived cell lines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions\/Significance<\/jats:title>\n                    <jats:p>\n                      Our results highlight species-specific differences in ZIKV susceptibility across bat-derived cell lines, with both ZIKV strains replicating to high titers on\n                      <jats:italic>R. aegyptiacus<\/jats:italic>\n                      cells but not on\n                      <jats:italic>A. jamaicensis<\/jats:italic>\n                      cells. We detected pro-inflammatory transcriptomic signatures in\n                      <jats:italic>R. aegyptiacus<\/jats:italic>\n                      cells infected with both ZIKV lineages. Notably, both the Asian-American and African ZIKV lineages demonstrated some capacity for immune evasion and productive replication in the\n                      <jats:italic>R. aegyptiacus<\/jats:italic>\n                      cell line.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014065","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T18:43:37Z","timestamp":1772822617000},"page":"e0014065","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative susceptibility of Old World and New World bat cell lines to Zika virus: Insights into viral replication and inflammatory responses"],"prefix":"10.1371","volume":"20","author":[{"given":"Alexander J.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna C.","family":"Fagre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julianna","family":"Gilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Horton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Nick","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Rivero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahsan","family":"Karimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Speranza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Letko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4397-6156","authenticated-orcid":true,"given":"Stephanie N.","family":"Seifert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"issue":"3","key":"pntd.0014065.ref001","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/S1473-3099(17)30727-2","article-title":"Association between microcephaly, Zika virus infection, and other risk factors in Brazil: final report of a case-control study","volume":"18","author":"TVB de Ara\u00fajo","year":"2018","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"pntd.0014065.ref002","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.1002203","article-title":"Zika virus infection as a cause of congenital brain abnormalities and guillain-barr\u00e9 syndrome: systematic review","volume":"14","author":"F Krauer","year":"2017","journal-title":"PLoS Med"},{"issue":"15","key":"pntd.0014065.ref003","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1056\/NEJMra1808246","article-title":"Zika virus infection \u2014 after the pandemic","volume":"381","author":"D Musso","year":"2019","journal-title":"New England J Med"},{"key":"pntd.0014065.ref004","doi-asserted-by":"crossref","first-page":"8370","DOI":"10.1038\/s41598-021-87816-5","article-title":"Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)","volume":"11","author":"AC Fagre","year":"2021","journal-title":"Sci Rep"},{"key":"pntd.0014065.ref005","unstructured":"World Health Organization. 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