{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:09:59Z","timestamp":1790186999798,"version":"4.1.0"},"reference-count":92,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020968","name":"Boehringer Ingelheim Animal Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T002727\/1"],"award-info":[{"award-number":["BB\/T002727\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T002727\/1"],"award-info":[{"award-number":["BB\/T002727\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Fasciola hepatica<\/jats:italic>\n                    causes fasciolosis, a parasitic disease that poses significant animal and human health challenges. Control relies on flukicides, most of which are adulticides, with only triclabendazole effective against the pathogenic migratory juvenile. Classical neurotransmitter pathways are widely targeted by anthelmintics yet remain underexplored for flukicide development. Here we explore the importance of serotonin (5-HT) signaling in juvenile fluke.\n                    <jats:italic>In silico<\/jats:italic>\n                    analyses confirmed all\n                    <jats:italic>F. hepatica<\/jats:italic>\n                    life stages express a complete 5-HT signaling pathway encompassing genes encoding proteins for 5-HT synthesis, transport, and reuptake, as well as five putative 5-HT G protein-coupled receptors (GPCRs). Homology and binding motif analyses supported the presence of two 5-HT\n                    <jats:sub>1<\/jats:sub>\n                    (Fh5HT\n                    <jats:sub>1A<\/jats:sub>\n                    , Fh5HT\n                    <jats:sub>1B<\/jats:sub>\n                    ) and three 5-HT\n                    <jats:sub>7<\/jats:sub>\n                    (Fh5HT\n                    <jats:sub>7A<\/jats:sub>\n                    ,\n                    <jats:sub>-7B<\/jats:sub>\n                    ,\n                    <jats:sub>-7C<\/jats:sub>\n                    ) GPCRs. Immunocytochemistry and\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization revealed widespread neuronal expression of 5-HT, its synthetic enzyme tryptophan hydroxylase (FhTPH), and the GPCR Fh5HT\n                    <jats:sub>7C<\/jats:sub>\n                    . 5-HT addition stimulated juvenile fluke motility; consistent with this observation, serotonin reuptake inhibition, which causes 5-HT persistence at synaptic junctions, also enhanced juvenile movement. Silencing of FhTPH, a key enzyme in 5-HT synthesis, blunted juvenile motility, a phenotype reversed by the addition of 5-HT. Silencing the fluke vesicular monoamine transporter (FhVMAT), which packages 5-HT into synaptic vesicles, reduced juvenile motility, whilst silencing the 5-HT reuptake transporter (FhSERT) which recycles synaptic 5-HT increased juvenile motility and growth, consistent with 5-HT accumulation enhancing effects. Whilst combinatorial silencing of Fh5HT\n                    <jats:sub>1<\/jats:sub>\n                    receptors reduced fluke motility, silencing Fh5HT\n                    <jats:sub>7<\/jats:sub>\n                    receptors led to a greater reduction in motility. Exogenous addition of 5-HT partially rescued motility deficits of juveniles with silenced Fh5HT\n                    <jats:sub>1<\/jats:sub>\n                    receptors, but 5-HT excitation was abolished in Fh5HT\n                    <jats:sub>7<\/jats:sub>\n                    -RNAi juveniles, exposing their importance to fluke motility. Notably, sustained 5-HT exposure promoted juvenile growth, but these effects were not blunted by receptor-RNAi. The findings emphasize a central role of serotonin signaling in both juvenile motility and growth, exposing novel aspects of receptor function and encouraging therapeutic exploitation for liver fluke control.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014097","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:29:26Z","timestamp":1774546166000},"page":"e0014097","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Serotonin signaling modulates growth and motility in juvenile Fasciola 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