{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:39:11Z","timestamp":1791686351076,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014134","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000}}],"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["20240101017JC"],"award-info":[{"award-number":["20240101017JC"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Scientific and Technology Project of Inner Mongolia Autonomous Region","award":["2021ZD0006"],"award-info":[{"award-number":["2021ZD0006"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hemorrhagic fever with renal syndrome (HFRS) is highly prevalent in northeastern China, especially along the borders with Russia and North Korea. However, the prevalence of HFRS-causing orthohantaviruses in rodents in these regions remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Rodents were captured across the tri-border region. Lung tissues were collected to detect human-pathogenic Hantaan virus (HTNV), Seoul virus (SEOV), and Amur virus (AMRV). Complete viral genome sequences from positive samples were amplified for phylogenetic and homology analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 430 rodents from four species were captured from five villages in the tri-border region, with\n                      <jats:italic>Apodemus agrarius<\/jats:italic>\n                      as the dominant species, accounting for 41.4%. HTNV was detected in 8.4% of\n                      <jats:italic>A. agrarius<\/jats:italic>\n                      and 3.6% of\n                      <jats:italic>R. norvegicus<\/jats:italic>\n                      individuals, with viral loads ranging from 2.2\u20136.9 log\u2081\u2080 copies\/\u03bcL and 4.1\u20136.9 log\u2081\u2080 copies\/\u03bcL, respectively. AMRV was identified in 6.3% of\n                      <jats:italic>A. peninsulae<\/jats:italic>\n                      individuals, with viral loads between 3.6\u20136.7 log\u2081\u2080 copies\/\u03bcL. SEOV was not detected in any samples. No significant differences in viral prevalence were observed across years, seasons, or collection habitats, nor in viral load between virus species or rodent species. One AMRV and ten HTNV complete genome sequences were successfully obtained. Phylogenetic analysis revealed that the identified HTNV strains formed a distinct clade with previously reported strains from northeastern China and Russia, showing nucleotide and amino acid similarities of 87.5\u201397.6% and 97.1\u2013100.0%, respectively. AMRV exhibited a close evolutionary relationship with strains isolated from patients and from\n                      <jats:italic>A. peninsulae<\/jats:italic>\n                      in China, displaying nucleotide and amino acid similarities of 80.6\u201384.7% and 92.3\u201397.7%, respectively, compared to HTNV.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our study confirms natural infections of HTNV in\n                      <jats:italic>A. agrarius<\/jats:italic>\n                      and\n                      <jats:italic>R. norvegicus<\/jats:italic>\n                      , and of AMRV in\n                      <jats:italic>A. peninsulae<\/jats:italic>\n                      , in the tri-border region of China, Russia, and North Korea. Further epidemiological studies on orthohantavirus infections in humans and rodents are warranted in this border region.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014134","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T17:47:26Z","timestamp":1776707246000},"page":"e0014134","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["A molecular survey of orthohantaviruses in rodents across the tri-border region of China, Russia, and North Korea"],"prefix":"10.1371","volume":"20","author":[{"given":"Yunzhi","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiru","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1980-6085","authenticated-orcid":true,"given":"Zhanli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianting","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-6944","authenticated-orcid":true,"given":"Zedong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"issue":"8","key":"pntd.0014134.ref001","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.3390\/v15081705","article-title":"Zoonotic Hantaviridae with Global Public Health Significance","volume":"15","author":"R-X Chen","year":"2023","journal-title":"Viruses"},{"key":"pntd.0014134.ref002","article-title":"Hantavirus infections","author":"T Av\u0161i\u010d-\u017dupanc","year":"2019","journal-title":"Clin Microbiol Infect"},{"issue":"2","key":"pntd.0014134.ref003","article-title":"Clinical study of critical patients with hemorrhagic fever with renal syndrome complicated by acute respiratory distress syndrome","volume":"9","author":"H Du","year":"2014","journal-title":"PLoS One"},{"issue":"11","key":"pntd.0014134.ref004","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003344","article-title":"Spatiotemporal transmission dynamics of hemorrhagic fever with renal syndrome in China, 2005-2012","volume":"8","author":"WY Zhang","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014134.ref005","first-page":"1","article-title":"Hemorrhagic Fever with Renal Syndrome: Pathogenesis and Clinical Picture","volume":"6","author":"H Jiang","year":"2016","journal-title":"Front Cell Infect Microbiol"},{"key":"pntd.0014134.ref006","doi-asserted-by":"crossref","DOI":"10.2196\/42673","article-title":"The Spatiotemporal Pattern and Its Determinants of Hemorrhagic Fever With Renal Syndrome in Northeastern China: Spatiotemporal Analysis","volume":"9","author":"Y Wang","year":"2023","journal-title":"JMIR Public Health Surveill"},{"issue":"5","key":"pntd.0014134.ref007","first-page":"3","article-title":"Detection and analysis of hantavirus nucleic acid in the cases of hemorrhagic fever with renal syndrome serum in Yanbian Korean Autonomous Prefecture","volume":"14","author":"B Shen","year":"2015","journal-title":"Chin J Public Health Eng"},{"issue":"03","key":"pntd.0014134.ref008","first-page":"167","article-title":"Isolation and identification of three strains of Seoul virus in Hunchun City of Jilin Province","volume":"7","author":"B Shen","year":"2008","journal-title":"Chin J of Public Health Eng"},{"key":"pntd.0014134.ref009","unstructured":"National Health Commission of the People\u2019s Republic of China. 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