{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:38:00Z","timestamp":1792701480706,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014144","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000}}],"reference-count":91,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","award":["CPT 2022-55050"],"award-info":[{"award-number":["CPT 2022-55050"]}],"id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Insecticide spraying is a common strategy for controlling dengue outbreaks, but its effectiveness is compromised by the development of resistance in mosquito populations. In this study, we subjected a strain of\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    known for its exceptional ability to develop resistance to controlled permethrin and lambda-cyhalothrin insecticides pressure using two different concentrations. We analyzed resistance mechanisms that are enhanced at each concentration and used RNA sequencing to identify transcripts specifically associated with these exposure levels. Our objective was to uncover the molecular mechanisms triggered by different insecticide concentrations and to distinguish responses between type I and type II pyrethroids, which differ in chemical structure. Our results showed that\n                    <jats:italic>kdr<\/jats:italic>\n                    mutations confer only moderate levels of resistance, as do detoxifying enzymes. For lambda-cyhalothrin, we identified genes involved in the electron transport chain, mitochondrial function, and overall responses to oxidative stress. tRNA transcripts were also upregulated, along with mitochondrial and stress-response transcripts, suggesting a metabolic shift, particularly toward maintaining homeostasis under oxidative stress. These changes point to mechanisms that sustain resistance to this type II insecticide beyond direct detoxification in this population. On the contrary, permethrin induced marked overexpression of cuticle genes, CYP450 genes (especially CYP4), and Odorant Binding Proteins. These expression patterns, together with metabolic enzymes, point to detoxification, reduced penetration, or even sequestration of insecticide, all of which intensify with increasing concentrations. This overregulation of genes suggests an integrated response complemented by classical metabolic detoxification and accompanied by overregulation of mitochondrial complexes. We showed that despite the shared mode of action of the insecticides permethrin and lambda-cyhalothrin, they elicit distinct responses in this\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    population. We also showed that the transcriptomic response depends on insecticide concentration and may modulate insecticide tolerance. This article advances understanding of the complexity of pyrethroid resistance in\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    and underscores the importance of considering both the insecticide type and the concentration used in vector control programs.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014144","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:45:49Z","timestamp":1790271949000},"page":"e0014144","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Resistance to pyrethroids in Aedes aegypti: Insights into transcriptomic response to different insecticide concentrations"],"prefix":"10.1371","volume":"20","author":[{"given":"Alejandro Mej\u00eda","family":"Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Universidad de Antioquia, UdeA","place":["Medell\u00edn, Colombia"],"department":["Grupo 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Microbiology, Immunology and Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8031-0225","authenticated-orcid":true,"given":"Omar","family":"Triana-Ch\u00e1vez","sequence":"additional","affiliation":[{"name":"Universidad de Antioquia, UdeA","place":["Medell\u00edn, Colombia"],"department":["Grupo Biolog\u00eda y Control de Enfermedades Infecciosas \u2013 BCEI"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"pntd.0014144.ref001","volume-title":"Managing pesticides in agriculture and public health: A compendium of FAO and WHO guidelines and other resources","author":"FAO W","year":"2021","edition":"2"},{"key":"pntd.0014144.ref002","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.actatropica.2018.12.021","article-title":"Mechanisms of pyrethroid resistance in Aedes (Stegomyia) aegypti from 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