{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T22:51:36Z","timestamp":1778539896786,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014150","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000}}],"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2023A1515220111"],"award-info":[{"award-number":["2023A1515220111"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Technology Projects of Guangzhou","award":["2024A04J5119"],"award-info":[{"award-number":["2024A04J5119"]}]},{"name":"President Foundation of Zhujiang Hospital, Southern Medical University","award":["yzjj2022qn24"],"award-info":[{"award-number":["yzjj2022qn24"]}]},{"name":"Guangdong Provincial Clinical Research Center for Laboratory Medicine","award":["2023B110008"],"award-info":[{"award-number":["2023B110008"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp. infections can manifest in various clinical symptoms, from asymptomatic carriage to gastroenteritis, and even severe sepsis. Given the rapid progression of the disease and its potential to cause severe outcomes or trigger cluster outbreaks, making the detection of\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp. critically important. Although broth enrichment culture is considered the gold standard, it is time-consuming and involves multiple steps, making it difficult to meet urgent diagnostic needs. Hence, prompt and precise detection of\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp. is crucial not only for early diagnosis and effective treatment, but also for preventing transmission, controlling outbreaks, and screening asymptomatic\n                      <jats:italic>Salmonella<\/jats:italic>\n                      carrier.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study developed a clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR associated (Cas) -SE assay that integrated the advantages of the recombinase polymerase amplification (RPA) and CRISPR\/Cas13a system for detecting\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp. The clinical performance of CRISPR\/Cas13a-SE assay was evaluated by a cohort of 94 inpatients with diarrhea and three prospective studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The CRISPR\/Cas13a-SE assay can be completed within 60 minutes, and its limit of detection was 100 fg\/\u03bcL. Compared to the broth enrichment culture, the CRISPR\/Cas13a-SE assay demonstrated a sensitivity of 87.5% and a specificity of 98.8% in a cohort of 94 inpatients with diarrhea. In our prospective studies involved three distinct cohorts: 1,662 food handlers, 211 outpatients with diarrhea, and 154 inpatients with Gram-negative bacteremia. Compared with broth enrichment culture, CRISPR\/Cas13a-SE assay had a high concordance rate of 98.79% (1,642\/1,662), 99.52% (210\/211), and 100.00% (154\/154) respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We demonstrated that the CRISPR\/Cas13a-SE system showed excellent detection performance for infectious diarrhea caused by\n                      <jats:italic>Salmonella<\/jats:italic>\n                      spp. The combined use of CRISPR\/Cas13a-SE with the blood culture method enhances the rapid diagnosis of invasive salmonellosis, which is crucial for early target-based therapy. Additionally, screening of asymptomatic\n                      <jats:italic>Salmonella<\/jats:italic>\n                      carrier will be benefit for disease prevention and control.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014150","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T17:28:08Z","timestamp":1774286888000},"page":"e0014150","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Application of CRISPR\/Cas13a system on the rapid detection of Salmonella spp."],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4050-2876","authenticated-orcid":true,"given":"Yongxin","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenli","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenyi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changhan","family":"Ai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiqing","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3887-2600","authenticated-orcid":true,"given":"Lingxiao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"pntd.0014150.ref001","volume-title":"Salmonella. 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