{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T18:54:34Z","timestamp":1775156074267,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014182","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000}}],"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Yaws, caused by\n                      <jats:italic>Treponema pallidum<\/jats:italic>\n                      subsp.\n                      <jats:italic>pertenue<\/jats:italic>\n                      (TP) is targeted for eradication by 2030 under the World Health Organization\u2019s (WHO) initiative, which relies on mass drug administration (MDA) of azithromycin. Evidence of persistent lesions after treatment has been reported. While the occurrences of\n                      <jats:italic>Haemophilus ducreyi<\/jats:italic>\n                      (HD) in yaws-like lesions has been widely documented, there is limited evidence regarding its influence on treatment outcomes assessed at the WHO-recommended 4-week follow-up. In this study, we sought to detect the occurrence of\n                      <jats:italic>H. ducreyi<\/jats:italic>\n                      in yaws-confirmed cases and assess its influence on treatment outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      We conducted a prospective cohort study of school children from Wassa Amenfi East and Aowin Districts in Ghana. A total of 46 Dual Path Platform (DPP) positive yaws cases were subjected to PCR analysis to determine the aetiological agents, including\n                      <jats:italic>T. pallidum<\/jats:italic>\n                      and\/or\n                      <jats:italic>H. ducreyi.<\/jats:italic>\n                      Treatment was with a single dose of oral azithromycin and outcomes were evaluated 4 weeks post-treatment, assessing clinical resolution and time to healing, with results stratified by the identified aetiological agents.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>Of the 46 participants tested, 18\/46 (39%) were positive for HD, 6 (13%) were TP positive, 3 (7%) were positive for TP\/HD, and 19 (41%) were negative for both pathogens tested. Healing rate was 80.4% (95% Confidence Interval [CI], 73.9\u201395.5) for all cases; 72.2% (95% CI: 57.0\u201393.4) for HD only, 83.3% (95% CI: 43.6\u201397.0) for TP only, 100% (95% CI: 43.9\u2013100) for TP\/HD, and 84.2% (95% CI: 62.4\u201394.5) for negative cases. Complete healing was generally observed by day 15\u201320.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results support the ongoing use of a single dose azithromycin in yaws elimination programmes, as evidenced by the healing rates observed. Nonetheless, greater focus should be directed toward improving diagnostic and treatment approaches for individual patients. Further research is necessary to better understand the aetiology of cutaneous ulcers in yaws-endemic regions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014182","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T17:35:09Z","timestamp":1774892109000},"page":"e0014182","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of the role of Haemophilus ducreyi coinfection on outcomes of Yaws treatment in the southwestern part of Ghana"],"prefix":"10.1371","volume":"20","author":[{"given":"Abigail","family":"Agbanyo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael Ntiamoah","family":"Oppong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dzifa Kofi","family":"Ahiatrogah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth Dede","family":"Tuwor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clement","family":"Tettey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Azabire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Owusu","family":"Boakye Yiadom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis Odai","family":"Laryea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Owusu-Ofori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard Odame","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4642-789X","authenticated-orcid":true,"given":"Yaw Ampem","family":"Amoako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"pntd.0014182.ref001","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1177\/0956462414549036","article-title":"Yaws","volume":"26","author":"M Marks","year":"2015","journal-title":"Int J STD AIDS"},{"issue":"3","key":"pntd.0014182.ref002","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006303","article-title":"Community-based mass treatment with azithromycin for the elimination of yaws in Ghana-Results of a pilot study","volume":"12","author":"AA Abdulai","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014182.ref003","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0140-6736(12)62130-8","article-title":"Yaws","volume":"381","author":"O Mitj\u00e0","year":"2013","journal-title":"Lancet"},{"key":"pntd.0014182.ref004","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1093\/bmb\/ldu037","article-title":"Yaws","volume":"113","author":"M Marks","year":"2015","journal-title":"Br Med Bull"},{"issue":"1","key":"pntd.0014182.ref005","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1128\/CMR.00070-13","article-title":"The endemic treponematoses","volume":"27","author":"L Giacani","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"pntd.0014182.ref006","volume-title":"Ending the Neglect to Attain the Sustainable Development Goals: A Road Map for Neglected Tropical Diseases 2021-2030","author":"WHO","year":"2021"},{"key":"pntd.0014182.ref007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0012899","article-title":"Guidance for conducting and evaluating serological surveys to assess interruption of yaws transmission in the context of an eradication target","volume":"19","author":"O Mitj\u00e0","year":"2025","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014182.ref008","unstructured":"W. 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Revised Declaration of Helsinki adopted by the global medical community, strengthening ethical standards in clinical research involving humans \u2013 WMA \u2013 The World Medical Association 2024. https:\/\/www.wma.net\/news-post\/revised-declaration-of-helsinki-adopted-by-the-global-medical-community-strengthening-ethical-standards-in-clinical-research-involving-humans\/ (accessed July 13, 2025)."},{"key":"pntd.0014182.ref026","author":"Ghana Statistical Service","year":"2021"},{"key":"pntd.0014182.ref027","unstructured":"Aowin Municipal Assembly. Republic of Ghana Composite Budget for 2023-2026 Programme Based Budget Estimates for 2023. 2023."},{"key":"pntd.0014182.ref028","year":"2023"},{"key":"pntd.0014182.ref029","unstructured":"WHO. YAWS: Recognition booklet for communities 2014. https:\/\/www.who.int\/publications\/i\/item\/9789241504096 (accessed May 20, 2025)."},{"key":"pntd.0014182.ref030","unstructured":"Chembio Diagnostics Inc. DPP\u00ae Syphilis Screen & Confirm Europe 2009. https:\/\/chembio.com\/products\/dpp-syphilis-screen-confirm-europe\/ (accessed March 17, 2025)."},{"key":"pntd.0014182.ref031","author":"World Health Organization","year":"2018"},{"key":"pntd.0014182.ref032","unstructured":"Qiagen. 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