{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:57:07Z","timestamp":1787021827639,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014185","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["75D30124C20324"],"award-info":[{"award-number":["75D30124C20324"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Anthropogenic factors have contributed to the expansion of the dengue vector\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    into previously non-endemic regions. In East Africa, rising dengue cases highlight gaps in understanding the role of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    . Here, we present a molecular characterization of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    in Somaliland following a dengue outbreak. Adult\n                    <jats:italic>Aedes<\/jats:italic>\n                    mosquitoes were collected from Hargeisa, Berbera and Burao. PCR and sequencing were applied to determine species, identify\n                    <jats:italic>vgsc<\/jats:italic>\n                    resistance mutations, and analyze bloodmeal sources. WHO bioassays were also conducted to determine the status of pyrethroid insecticide resistance. Multiple\n                    <jats:italic>COI<\/jats:italic>\n                    haplotypes were identified, with Hargeisa exhibiting the highest diversity. Pyrethroid resistance mutations S989P, V1016G, and F1534C were detected at all locations, with the highest frequency observed in Burao. While no association was observed between allele frequencies and insecticide resistance, resistance was associated with total heterozygous genotypes. The detection of S989P\/V1016G mutation combinations, observed mostly in Asia underscores the need for investigations into the origin of uncommon\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    lineages into East Africa. Overall, this study reveals the varying utility of molecular markers for species identification and tracking phylogeographic shifts in the distribution of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    lineages. This highlights the need for improved vector control strategies and strengthen surveillance in Somaliland.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014185","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T17:50:36Z","timestamp":1776707436000},"page":"e0014185","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular investigation of diverse Aedes aegypti in heightened dengue transmission settings in Somaliland, 2023\u20132024"],"prefix":"10.1371","volume":"20","author":[{"given":"Babatunde","family":"Oriyomi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9438-9541","authenticated-orcid":true,"given":"Tamar E.","family":"Carter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"issue":"9","key":"pntd.0014185.ref001","doi-asserted-by":"crossref","DOI":"10.1289\/ehp.116-a382","article-title":"Dengue reborn: widespread resurgence of a resilient vector","volume":"116","author":"ML Phillips","year":"2008","journal-title":"Environ Health Perspect"},{"issue":"2","key":"pntd.0014185.ref002","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/MOP.0000000000001229","article-title":"Dengue, chikungunya and zika arbovirus infections in Caribbean children","volume":"35","author":"CDC Christie","year":"2023","journal-title":"Curr Opin Pediatr"},{"issue":"10427","key":"pntd.0014185.ref003","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/S0140-6736(23)02576-X","article-title":"Dengue","volume":"403","author":"G 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