{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:15:06Z","timestamp":1778177706495,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014222","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000}}],"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["grant no. 14A1-MRPP04-014"],"award-info":[{"award-number":["grant no. 14A1-MRPP04-014"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Global trade and climate change are driving the geographic expansion of dengue vectors, contributing to the global spread of dengue. Conventional vector control measures have proven insufficient to prevent substantial disease burdens, highlighting the need for innovative and sustainable strategies. The release of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -infected mosquitoes offers a promising alternative for dengue suppression. Here, we developed a locally derived\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    line carrying the\n                    <jats:italic>w<\/jats:italic>\n                    AlbB strain (\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-Tw-Kao) and systematically evaluated its fitness, viral interference, and potential for vector population control. The strain was generated through embryonic microinjection of cytoplasm containing the intact\n                    <jats:italic>w<\/jats:italic>\n                    AlbB endosymbiont from field-collected\n                    <jats:italic>Ae. albopictus<\/jats:italic>\n                    in Kaohsiung, Taiwan, resulting in a stably infected mosquito line with 100% maternal transmission. Whole-genome sequencing confirmed a high similarity to the reference\n                    <jats:italic>w<\/jats:italic>\n                    AlbB genome. Cross-mating experiments demonstrated complete cytoplasmic incompatibility (CI, 0% egg hatch) when\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-Tw-Kao males were mated with uninfected females. Antiviral assays against dengue virus serotype 2 (DENV-2) and Zika virus showed significant reductions in viral titers in the midgut, salivary glands, and saliva. In cage experiments, increasing release ratios of\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-Tw-Kao males led to significant suppression of wild-type populations, achieving up to approximately 90% reduction in egg hatch. These findings demonstrate the successful development of a locally derived\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-infected\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    line with strong CI, stable maternal transmission, and effective DENV and ZIKV blocking. These properties provide a foundation for future field-relevant evaluation under both suppression and replacement deployment frameworks.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014222","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:47:08Z","timestamp":1777916828000},"page":"e0014222","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and characterization of a Wolbachia (wAlbB)-infected Aedes aegypti line (Tw-Kao) for dengue control"],"prefix":"10.1371","volume":"20","author":[{"given":"Hui-Ying","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-Tsong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Kang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Hao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung-Chieh","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Hsiu","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Sheng","family":"Yeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-Guang","family":"Goh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinjun","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pui-Kuan","family":"Toh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sui-Hann","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Chiuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1159-1286","authenticated-orcid":true,"given":"Wei-Liang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"issue":"7446","key":"pntd.0014222.ref001","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"pntd.0014222.ref002","doi-asserted-by":"crossref","DOI":"10.1038\/nrmicro2460","article-title":"Dengue: a continuing global threat","volume":"8","author":"MG Guzman","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"pntd.0014222.ref003","unstructured":"WHO. Dengue and severe dengue; 2022 [cited 2022 Mar 6]. Available from: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dengue-and-severe-dengue"},{"issue":"13","key":"pntd.0014222.ref004","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1056\/NEJMoa1506223","article-title":"Efficacy and long-term safety of a dengue vaccine in regions of endemic disease","volume":"373","author":"SR Hadinegoro","year":"2015","journal-title":"N Engl J Med"},{"key":"pntd.0014222.ref005","doi-asserted-by":"crossref","first-page":"4848","DOI":"10.1016\/j.vaccine.2018.09.063","article-title":"Dengue vaccine: WHO position paper, September 2018 - recommendations","volume":"37","year":"2019","journal-title":"Vaccine"},{"issue":"51","key":"pntd.0014222.ref006","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abq7345","article-title":"Discovery of super-insecticide-resistant dengue mosquitoes in Asia: threats of concomitant knockdown resistance mutations","volume":"8","author":"S Kasai","year":"2022","journal-title":"Sci Adv"},{"key":"pntd.0014222.ref007","doi-asserted-by":"crossref","first-page":"105710","DOI":"10.1016\/j.pestbp.2023.105710","article-title":"Correlation between pyrethroid knockdown resistance and mutation frequency of voltage-gated sodium channel and its application in Aedes aegypti management","volume":"198","author":"H-H Lin","year":"2024","journal-title":"Pestic Biochem Physiol"},{"issue":"7","key":"pntd.0014222.ref008","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.tig.2022.02.013","article-title":"Symbionts and gene drive: two strategies to combat vector-borne disease","volume":"38","author":"G-H Wang","year":"2022","journal-title":"Trends Genet"},{"key":"pntd.0014222.ref009","doi-asserted-by":"crossref","first-page":"107410","DOI":"10.1016\/j.actatropica.2024.107410","article-title":"Wolbachia-based emerging strategies for control of vector-transmitted disease","volume":"260","author":"D Montenegro","year":"2024","journal-title":"Acta Trop"},{"key":"pntd.0014222.ref010","article-title":"Development of a confinable gene drive system in the human disease vector Aedes aegypti","volume":"9","author":"M Li","year":"2020","journal-title":"Elife"},{"issue":"3","key":"pntd.0014222.ref011","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1038\/nrg.2015.34","article-title":"Cheating evolution: engineering gene drives to manipulate the fate of wild populations","volume":"17","author":"J Champer","year":"2016","journal-title":"Nat Rev Genet"},{"issue":"2","key":"pntd.0014222.ref012","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1574-6968.2008.01110.x","article-title":"How many species are infected with Wolbachia?--A statistical analysis of current data","volume":"281","author":"K Hilgenboecker","year":"2008","journal-title":"FEMS Microbiol Lett"},{"issue":"1469","key":"pntd.0014222.ref013","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1098\/rspb.2001.1593","article-title":"Transfection of Wolbachia in Lepidoptera: the feminizer of the adzuki bean borer Ostrinia scapulalis causes male killing in the Mediterranean flour moth Ephestia kuehniella","volume":"268","author":"Y Fujii","year":"2001","journal-title":"Proc Biol Sci"},{"issue":"6","key":"pntd.0014222.ref014","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.chom.2021.03.006","article-title":"Living in the endosymbiotic world of Wolbachia: a centennial review","volume":"29","author":"R Kaur","year":"2021","journal-title":"Cell Host Microbe"},{"issue":"1972","key":"pntd.0014222.ref015","first-page":"20212781","article-title":"Sex determination systems as the interface between male-killing bacteria and their hosts","volume":"289","author":"EA Hornett","year":"2022","journal-title":"Proc Biol Sci"},{"issue":"10","key":"pntd.0014222.ref016","doi-asserted-by":"crossref","first-page":"4519","DOI":"10.1111\/1462-2920.16125","article-title":"Toward an accurate mechanistic understanding of Wolbachia-induced cytoplasmic incompatibility","volume":"24","author":"W Wang","year":"2022","journal-title":"Environ Microbiol"},{"key":"pntd.0014222.ref017","article-title":"Harnessing mosquito-Wolbachia symbiosis for vector and disease control","author":"K Bourtzis","year":"2014","journal-title":"Acta Trop"},{"issue":"8","key":"pntd.0014222.ref018","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1038\/s41587-020-0649-2","article-title":"Author Correction: Efficient production of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations","volume":"38","author":"JE Crawford","year":"2020","journal-title":"Nat Biotechnol"},{"issue":"7767","key":"pntd.0014222.ref019","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41586-019-1407-9","article-title":"Incompatible and sterile insect techniques combined eliminate mosquitoes","volume":"572","author":"X Zheng","year":"2019","journal-title":"Nature"},{"issue":"41","key":"pntd.0014222.ref020","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2106828118","article-title":"Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia","volume":"118","author":"NW Beebe","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7361","key":"pntd.0014222.ref021","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/nature10356","article-title":"Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission","volume":"476","author":"AA Hoffmann","year":"2011","journal-title":"Nature"},{"issue":"10","key":"pntd.0014222.ref022","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007771","article-title":"Combined sterile insect technique and incompatible insect technique: the first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand","volume":"13","author":"P Kittayapong","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"5","key":"pntd.0014222.ref023","article-title":"Efficacy of Wolbachia-mediated sterility to reduce the incidence of dengue: a synthetic control study in Singapore","volume":"5","author":"JT Lim","year":"2024","journal-title":"Lancet Microbe"},{"issue":"7361","key":"pntd.0014222.ref024","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/nature10355","article-title":"The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations","volume":"476","author":"T Walker","year":"2011","journal-title":"Nature"},{"key":"pntd.0014222.ref025","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.12688\/gatesopenres.13061.2","article-title":"Establishment of wMel Wolbachia in Aedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia","volume":"3","author":"PA Ryan","year":"2020","journal-title":"Gates Open Res"},{"issue":"7","key":"pntd.0014222.ref026","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.cell.2009.11.042","article-title":"A Wolbachia symbiont in Aedes aegypti limits infection with dengue, Chikungunya, and Plasmodium","volume":"139","author":"LA Moreira","year":"2009","journal-title":"Cell"},{"issue":"6","key":"pntd.0014222.ref027","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.chom.2016.04.021","article-title":"Wolbachia blocks currently circulating Zika virus isolates in Brazilian Aedes aegypti mosquitoes","volume":"19","author":"HLC Dutra","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"10","key":"pntd.0014222.ref028","article-title":"Wolbachia wAlbB inhibit dengue and Zika infection in the mosquito Aedes aegypti with an Australian background","volume":"16","author":"LE Hugo","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"pntd.0014222.ref029","doi-asserted-by":"crossref","DOI":"10.1093\/pnasnexus\/pgac203","article-title":"Wolbachia wAlbB remains stable in Aedes aegypti over 15 years but exhibits genetic background-dependent variation in virus blocking","volume":"1","author":"X Liang","year":"2022","journal-title":"PNAS Nexus"},{"issue":"1","key":"pntd.0014222.ref030","article-title":"The Wolbachia strain wAu provides highly efficient virus transmission blocking in Aedes aegypti","volume":"14","author":"TH Ant","year":"2018","journal-title":"PLoS Pathog"},{"issue":"24","key":"pntd.0014222.ref031","doi-asserted-by":"crossref","DOI":"10.1016\/j.cub.2019.11.007","article-title":"Establishment of Wolbachia strain wAlbB in Malaysian populations of Aedes aegypti for dengue control","volume":"29","author":"WA Nazni","year":"2019","journal-title":"Curr Biol"},{"issue":"23","key":"pntd.0014222.ref032","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1056\/NEJMoa2030243","article-title":"Efficacy of Wolbachia-infected mosquito deployments for the control of dengue","volume":"384","author":"A Utarini","year":"2021","journal-title":"N Engl J Med"},{"key":"pntd.0014222.ref033","doi-asserted-by":"crossref","first-page":"50","DOI":"10.12688\/gatesopenres.13122.1","article-title":"Reduced dengue incidence following deployments of Wolbachia-infected Aedes aegypti in Yogyakarta, Indonesia: a quasi-experimental trial using controlled interrupted time series analysis","volume":"4","author":"C Indriani","year":"2020","journal-title":"Gates Open Res"},{"issue":"2","key":"pntd.0014222.ref034","doi-asserted-by":"crossref","first-page":"108942","DOI":"10.1016\/j.isci.2024.108942","article-title":"Introduction of Aedes aegypti mosquitoes carrying wAlbB Wolbachia sharply decreases dengue incidence in disease hotspots","volume":"27","author":"AA Hoffmann","year":"2024","journal-title":"iScience"},{"issue":"7","key":"pntd.0014222.ref035","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009556","article-title":"Effectiveness of Wolbachia-infected mosquito deployments in reducing the incidence of dengue and other Aedes-borne diseases in Niter\u00f3i, Brazil: a quasi-experimental study","volume":"15","author":"SB Pinto","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"issue":"3","key":"pntd.0014222.ref036","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0012936","article-title":"Correction: Large-scale releases and establishment of wMel Wolbachia in Aedes aegypti mosquitoes throughout the Cities of Bello, Medell\u00edn and Itag\u00fc\u00ed, Colombia","volume":"19","author":"I Dar\u00edo Velez","year":"2025","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"pntd.0014222.ref037","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.3390\/microorganisms11081911","article-title":"First detection and genetic identification of wolbachia endosymbiont in field-caught Aedes aegypti (Diptera: Culicidae) mosquitoes collected from southern Taiwan","volume":"11","author":"L-L Chao","year":"2023","journal-title":"Microorganisms"},{"issue":"5746","key":"pntd.0014222.ref038","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1126\/science.1117607","article-title":"Wolbachia establishment and invasion in an Aedes aegypti laboratory population","volume":"310","author":"Z Xi","year":"2005","journal-title":"Science"},{"issue":"8","key":"pntd.0014222.ref039","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/j.ibmb.2005.03.015","article-title":"Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection","volume":"35","author":"Z Xi","year":"2005","journal-title":"Insect Biochem Mol Biol"},{"issue":"14","key":"pntd.0014222.ref040","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"T Seemann","year":"2014","journal-title":"Bioinformatics"},{"issue":"22","key":"pntd.0014222.ref041","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"AJ Page","year":"2015","journal-title":"Bioinformatics"},{"issue":"9","key":"pntd.0014222.ref042","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"A Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"pntd.0014222.ref043","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/s41467-024-45238-7","article-title":"Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti","volume":"15","author":"CJ McNamara","year":"2024","journal-title":"Nat Commun"},{"issue":"7","key":"pntd.0014222.ref044","article-title":"Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells","volume":"6","author":"P Lu","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014222.ref045","doi-asserted-by":"crossref","first-page":"15042","DOI":"10.1073\/pnas.0403853101","article-title":"Wolbachia-induced cytoplasmic incompatibility as a means for insect pest population control","volume":"101","author":"S Zabalou","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"pntd.0014222.ref046","doi-asserted-by":"crossref","first-page":"724345","DOI":"10.3389\/fmicb.2021.724345","article-title":"Blood meals with active and heat-inactivated serum modifies the gene expression and microbiome of Aedes albopictus","volume":"12","author":"A Calle-Tob\u00f3n","year":"2021","journal-title":"Front Microbiol"},{"issue":"18","key":"pntd.0014222.ref047","doi-asserted-by":"crossref","first-page":"6584","DOI":"10.3390\/ijms21186584","article-title":"Transcriptome of the Aedes aegypti mosquito in response to human complement proteins","volume":"21","author":"GI Giraldo-Calder\u00f3n","year":"2020","journal-title":"Int J Mol Sci"},{"key":"pntd.0014222.ref048","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s42003-017-0011-5","article-title":"Synthetic miRNAs induce dual arboviral-resistance phenotypes in the vector mosquito Aedes aegypti","volume":"1","author":"PS Yen","year":"2018","journal-title":"Commun Biol"},{"issue":"9","key":"pntd.0014222.ref049","doi-asserted-by":"crossref","first-page":"101486","DOI":"10.1016\/j.isci.2020.101486","article-title":"C-type lectins link immunological and reproductive processes in Aedes aegypti","volume":"23","author":"H-H Li","year":"2020","journal-title":"iScience"},{"issue":"1","key":"pntd.0014222.ref050","doi-asserted-by":"crossref","first-page":"12535","DOI":"10.1038\/s41598-018-30968-8","article-title":"Experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant Aedes aegypti","volume":"8","author":"L Gray","year":"2018","journal-title":"Sci Rep"},{"issue":"1","key":"pntd.0014222.ref051","article-title":"Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain","volume":"16","author":"W-L Liu","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014222.ref052","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1093\/jee\/64.4.869","article-title":"Determination of sterile-insect competitiveness","volume":"64","author":"M Fried","year":"1971","journal-title":"J Econ Entomol"},{"key":"pntd.0014222.ref053","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2022"},{"issue":"4","key":"pntd.0014222.ref054","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.00128-24","article-title":"Wolbachia strains wMel and wAlbB differentially affect Aedes aegypti traits related to fecundity","volume":"12","author":"R Maciel-de-Freitas","year":"2024","journal-title":"Microbiol Spectr"},{"key":"pntd.0014222.ref055","doi-asserted-by":"crossref","first-page":"947857","DOI":"10.3389\/fmicb.2022.947857","article-title":"Studies on the fitness characteristics of wMel- and wAlbB-introgressed Aedes aegypti (Pud) lines in comparison with wMel- and wAlbB-transinfected Aedes aegypti (Aus) and wild-type Aedes aegypti (Pud) lines","volume":"13","author":"C Sadanandane","year":"2022","journal-title":"Front Microbiol"},{"issue":"3","key":"pntd.0014222.ref056","doi-asserted-by":"crossref","first-page":"507","DOI":"10.4269\/ajtmh.15-0608","article-title":"Fitness of wAlbB Wolbachia infection in Aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion","volume":"94","author":"JK Axford","year":"2016","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"pntd.0014222.ref057","doi-asserted-by":"crossref","first-page":"13425","DOI":"10.1038\/s41598-025-98093-x","article-title":"Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB","volume":"15","author":"HM Al-Amin","year":"2025","journal-title":"Sci Rep"},{"issue":"2","key":"pntd.0014222.ref058","article-title":"A decade of stability for wMel Wolbachia in natural Aedes aegypti populations","volume":"18","author":"PA Ross","year":"2022","journal-title":"PLoS Pathog"},{"issue":"2","key":"pntd.0014222.ref059","doi-asserted-by":"crossref","DOI":"10.1128\/aem.02350-24","article-title":"Development of the Incompatible Insect Technique targeting Aedes albopictus: introgression of a wild nuclear background restores the performance of males artificially infected with Wolbachia","volume":"91","author":"Q Lejarre","year":"2025","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"pntd.0014222.ref060","article-title":"Matching the genetics of released and local Aedes aegypti populations is critical to assure Wolbachia invasion","volume":"13","author":"A Garcia G de","year":"2019","journal-title":"PLoS Negl Trop Dis"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0014222","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0014222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T17:48:37Z","timestamp":1778176117000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0014222"}},"subtitle":[],"editor":[{"given":"Rafael","family":"Maciel-de-Freitas","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,5,4]]},"references-count":60,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0014222","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,4]]}}}