{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:09:07Z","timestamp":1782857347929,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014248","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000}}],"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dengue, a mosquito-borne virus continues to be a public health concern in the tropics and subtropical parts of Africa. Due to the increase of urbanization, climate change and trans-Atlantic trade, the transmitting vector, Aedes aegypti, has become prevalent hence the rapid growth of the disease, globally. In Ghana, there have been sporadic laboratory-confirmed cases of dengue reported over the years through surveillance activities. However, despite the detection of these cases, Ghana had never experienced a major outbreak (unlike its neighboring countries) until July 2024.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>During this outbreak, a total of 1471 suspected dengue fever specimen received from various health facilities in Ghana in NMIMR for molecular diagnostic testing using a RT-qPCR assay for dengue, chikungunya and Zika viruses and selected positives sequenced using Illumina Next Generation Sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Dengue fever virus RNA was detected from 206 samples and serotyped as DENV-1 with one DENV-3 coinfection. Thirty-nine genomes were successfully generated after sequencing and phylogenetic analysis of DENV-1 strains revealed two main clusters with AFI isolates in Ghana and isolates from other West African countries (C\u00f4te d\u2019Ivoire, Burkina Faso, Benin, and Senegal) circulating between 2017\u20132019. In 2023, DENV-1 was frequently isolated which could account for it being the predominant serotype transmitted in the recent outbreak.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The outbreak response and the case management procedures deployed by the health authorities during this outbreak were swift and was enough to prevent a fatal difficult-to-control situation. With the absence of a widely accepted commercialized vaccine and treatment for dengue fever, there is a need to enhance surveillance activities and control the vectors which can transmit DENV in-country to curb the occurrence of outbreaks.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014248","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T17:39:38Z","timestamp":1778521178000},"page":"e0014248","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Outbreak of dengue fever in Ghana: The emergence of DENV-1 serotype"],"prefix":"10.1371","volume":"20","author":[{"given":"Deborah","family":"Pratt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaw Awuku","family":"Larbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalene","family":"Ofori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bright","family":"Agbodzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Wiley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ama O. 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