{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:48:51Z","timestamp":1791571731591,"version":"4.3.4"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1182432"],"award-info":[{"award-number":["APP1182432"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP220102997"],"award-info":[{"award-number":["DP220102997"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["102591\/Z\/13\/Z"],"award-info":[{"award-number":["102591\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    The\n                    <jats:italic>w<\/jats:italic>\n                    Mel strain of the insect endosymbiont\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    reduces the potential for\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    to transmit mosquito-borne viruses such as dengue (DENV). Field trials that have introgressed\n                    <jats:italic>w<\/jats:italic>\n                    Mel into\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    populations have shown this approach significantly reduces dengue incidence. In a laboratory setting some\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    develop infectious saliva following a viremic blood meal. Additionally, studies have demonstrated that exposing\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    to heat treatment, particularly during the larval stage, reduces\n                    <jats:italic>w<\/jats:italic>\n                    Mel density in key tissues such as the ovaries, midgut and salivary glands. Here we build on these studies, using viremic blood collected from 13 dengue inpatients at the Hospital for Tropical Diseases in Ho Chi Minh City (Viet Nam), to assess how temperature affects the protection afforded to\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    by\n                    <jats:italic>w<\/jats:italic>\n                    Mel. We found that, compared to\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    reared at 28\u2009\u00b1\u20094\u00b0C, those reared at 31\u2009\u00b1\u20094\u00b0C developed infectious saliva more frequently, but the risk of this occurring was still reduced compared to WT mosquitoes reared at the same temperature. Heat treatment reduced the density of\n                    <jats:italic>w<\/jats:italic>\n                    Mel in all tissues tested, and significantly increased the amount of DENV-1 and DENV-4 replication in\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    . When comparing cohorts of\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    that did or did not develop infectious saliva, DENV levels in the head\/thorax were associated with increased odds of mosquitoes developing infectious saliva, but\n                    <jats:italic>w<\/jats:italic>\n                    Mel density was not. Overall, these findings show that elevated rearing temperatures increase the risk of patient-derived DENV breakthrough infections in\n                    <jats:italic>w<\/jats:italic>\n                    Mel-\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    , potentially due to increased DENV replication in these mosquitoes. This limitation suggests it would be prudent to increase surveillance in regions using\n                    <jats:italic>w<\/jats:italic>\n                    Mel for dengue control when daily mean temperatures remain above 30\u00b0C for multiday periods.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014255","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:50:13Z","timestamp":1791568213000},"page":"e0014255","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of temperature on patient-derived dengue virus breakthrough infections in wMel-infected Aedes aegypti"],"prefix":"10.1371","volume":"20","author":[{"given":"Daniela","family":"da Silva Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Oxford University Clinical Research Unit","place":["Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vi 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