{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T04:11:13Z","timestamp":1777954273563,"version":"3.51.4"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NHC Key Laboratory of Parasite and Vector Biology","award":["No. NHCKFKT2023-07"],"award-info":[{"award-number":["No. NHCKFKT2023-07"]}]},{"name":"the National Key Research and Development Program of China","award":["No. 2021YFC2300800, 2021YFC2300804"],"award-info":[{"award-number":["No. 2021YFC2300800, 2021YFC2300804"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Cystic echinococcosis (CE), a neglected zoonotic disease caused by the larval stage of\n                      <jats:italic>Echinococcus granulosus<\/jats:italic>\n                      , requires effective vaccine strategies for sustainable control. This study evaluated the immunogenic profiles of two antigenic targets: EgAgB8\/1, a dominant immunogenic component of hydatid cyst fluid, and Eg-01883, a protoscolex-specific antigen identified through bioinformatic screening.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/principal findings<\/jats:title>\n                    <jats:p>\n                      <jats:italic>E. granulosus<\/jats:italic>\n                      strains were isolated from infected dogs for genomic DNA extraction. Recombinant proteins rEgAgB8\/1 and rEg-01883 were expressed in\n                      <jats:italic>E. coli<\/jats:italic>\n                      , purified, and validated by SDS-PAGE and western blot. Initial protein microarray screening identified rEgAgB8\/1 as exhibiting markedly higher immunoreactivity with cystic echinococcosis (CE) patient sera compared to the minimally reactive Eg-01883. Based on this finding, subsequent investigation focused on rEgAgB8\/1 using a BALB\/c mouse immunization model. The recombinant protein elicited potent humoral immunity, with antigen-specific IgG titers reaching 1:16,000, and stimulated significant lymphocyte proliferation. Immunized mouse sera specifically recognized native EgAgB8\/1 in hydatid crude antigen preparations, confirming natural antigenicity. Flow cytometric analysis demonstrated that rEgAgB8\/1 immunization significantly expanded splenic plasmablasts, memory B cells, and T follicular helper cells. Furthermore, it enhanced IFN-\u03b3 production in both CD4\u207a and CD8\u2009\u207a\u2009T cells while maintaining baseline IL-10\u2009\u207a\u2009T cell frequencies, and induced robust T cell memory responses. Statistical analyses were performed using Student\u2019s t-test for comparative evaluation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings establish rEgAgB8\/1 as a highly immunogenic antigen capable of eliciting a cellular immune response characterized by dominant IFN-\u03b3 production without concomitant IL-10 elevation, alongside durable humoral responses in mice. The comprehensive immunogenicity profile supports further research into its immunological potential against cystic echinococcosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014260","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:38:15Z","timestamp":1777916295000},"page":"e0014260","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The hydatid cyst fluid protein EgAgB8\/1 demonstrates potent immunogenicity by eliciting robust humoral and cellular immune responses in 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