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Primary\n                      <jats:italic>Echinostoma caproni<\/jats:italic>\n                      infection disrupts epithelial metabolism, differentiation, and repair in an IL-25-deficient environment, as shown in a previous study by our research group; however, the adaptive mechanisms during homologous superimposed infections remain unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal findings<\/jats:title>\n                    <jats:p>Male ICR mice were assigned to control, primary infection, and homologous superimposed infection groups, and ileal epithelial cells were isolated for proteomic profiling using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) with data-dependent acquisition (DDA) and sequential window acquisition of all theoretical mass spectra (SWATH). Differential protein expression was analyzed with Elastic Net regression, partial least squares discriminant analysis, and fold-change ranking, while functional enrichment and protein\u2013protein interaction networks were explored using gene set enrichment analysis (GSEA) and STRING. Notably, homologous superimposed infection revealed proteomic signatures associated with lysosomal and peroxisomal lipid metabolism, PPAR pathway activation, cytoskeletal reorganization, epithelial barrier reinforcement, a specialized antimicrobial peptide repertoire, and interactions between IgE receptor-associated proteins, consistent with a restoration of intestinal homeostasis influenced by IL-25.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Host adaptation to repeated\n                      <jats:italic>E. caproni<\/jats:italic>\n                      exposure involves coordinated metabolic, signaling, and tissue repair responses that partially restore intestinal homeostasis, with IL-25 emerging as a central regulator linking metabolic reprogramming, epithelial integrity, and anti-helminth immunity, thereby providing a proteomic framework for understanding how repeated helminth exposure drives partial resistance through integrated epithelial and immunometabolic adaptations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014294","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:29:58Z","timestamp":1787592598000},"page":"e0014294","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic reprogramming of ileal epithelial cells during homologous superimposed intestinal trematode infection reveals coordinated restoration of intestinal 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