{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T18:06:13Z","timestamp":1780682773342,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014358","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000}}],"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2401005"],"award-info":[{"award-number":["2021YFC2401005"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Human brucellosis is re-emerging in the Chinese Southwest, and its epidemic characteristics remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>Descriptive epidemiology, Joinpoint regression, spatial autocorrelation, serological surveillance and pathogen analysis were adopted to clarify the epidemiological evolution of the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Between 2004 and 2024, 9,822 brucellosis cases occurred, dominated by 7,270 cases in Yunnan. The epidemic showed a persistent upward trend (AAPC\u2009\u22655.28,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), and 51 cities\/prefectures were identified with statistically significant increse (AAPC\u2009\u2265\u20099.30,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). Serological surveillance of 36,459 individuals revealed a seropositivity rate of 2.13% (95%CI, 2.00%, 2.26%), identifying 1,831 new cases. Sheep\/goats seropositivity at 1.02% (95%CI, 0.97%, 1.06%) and cattle at 0.45% (95%CI, 0.42%, 0.48%). Sustained elevated human incidence was validated by serological evidence, and seroprevalence trends in sheep\/goats aligned closely with human epidemic dynamics. Human brucellosis has evolved from random to significantly geographically and spatial aggregated, spreading continuously with intensified clustering in central-eastern Yunnan, the Sichuan basin and Tibet\u2019s Ngari-Qamdo axis, highlighting these as priority control zones. Human brucellosis has transitioned from sporadic to locally endemic, with phased progression and increasing geographical clustering recently. Human brucellosis in the Chinese Southwest is characterized by the persistent dominance of\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      bv. 3, primarily represented by the co-circulating MLVA-11 genotypes GT116 and GT125, which form a stable, widely disseminated lineage across the region.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      From the re-emergence to persistent endemicity of human brucellosis in study area, the extensive distribution of\n                      <jats:italic>B. melitensis<\/jats:italic>\n                      with unique genotyping reveals a stable zoonotic cycle that requires coordinated regional control.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014358","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:40:02Z","timestamp":1780076402000},"page":"e0014358","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatiotemporal dynamics and serosurveillance landscape of brucellosis at the human-animal interface in the Chinese Southwest: A retrospective study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6914-9825","authenticated-orcid":true,"given":"Qiuju","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Canjun","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4904-4878","authenticated-orcid":true,"given":"Zhiguo","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"issue":"2","key":"pntd.0014358.ref001","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"G Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"pntd.0014358.ref002","first-page":"742","article-title":"An overview of Brucellosis","volume":"20","author":"N Haque","year":"2011","journal-title":"Mymensingh Med J"},{"issue":"1","key":"pntd.0014358.ref003","doi-asserted-by":"crossref","first-page":"2290839","DOI":"10.1080\/22221751.2023.2290839","article-title":"Long ignored but making a comeback: a worldwide epidemiological evolution of human brucellosis","volume":"13","author":"Z Liu","year":"2024","journal-title":"Emerg Microbes Infect"},{"key":"pntd.0014358.ref004","unstructured":"Zhiguo L, Miao W, Yue S, Liping W, Xiang R, Zhenjun L, et al. 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