{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:36:06Z","timestamp":1793126166405,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014366","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:00:00Z","timestamp":1793059200000}}],"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022083","name":"VLIRUOS","doi-asserted-by":"publisher","award":["PE2018TEA470A102"],"award-info":[{"award-number":["PE2018TEA470A102"]}],"id":[{"id":"10.13039\/501100022083","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022083","name":"VLIRUOS","doi-asserted-by":"publisher","award":["PE2022TEA518A102"],"award-info":[{"award-number":["PE2022TEA518A102"]}],"id":[{"id":"10.13039\/501100022083","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      As malaria transmission declines in regions approaching elimination, highly sensitive diagnostic tools are needed to detect low-density infections, particularly in active and passive surveillance settings. Loop-mediated isothermal amplification (LAMP) is a promising molecular alternative to conventional diagnostics. This study evaluated the performance of a colorimetric LAMP (cLAMP) assay for detecting\n                      <jats:italic>Plasmodium vivax<\/jats:italic>\n                      and\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      from dried blood spots (DBS) in the Peruvian Amazon.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal findings<\/jats:title>\n                    <jats:p>The cLAMP assay was applied to DNA extracted from DBS collected through active case detection (ACD; n\u2009=\u2009574) and passive case detection (PCD; n\u2009=\u2009431). Two extraction methods were compared: mini spin column (MSC DNA) and a boil-and-spin (BS DNA) method. Diagnostic performance was evaluated against microscopy and real-time quantitative PCR (qPCR).<\/jats:p>\n                    <jats:p>\n                      In ACD samples, qPCR identified 125 malaria-positive cases (117\n                      <jats:italic>P. vivax<\/jats:italic>\n                      , 8\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      ). Microscopy showed a sensitivity of 57.6%, compared with 63.2% for cLAMP using BS DNA and 82.4% using MSC DNA. In PCD samples, qPCR detected 140 positives (129\n                      <jats:italic>P. vivax<\/jats:italic>\n                      , 9\n                      <jats:italic>P. falciparum,<\/jats:italic>\n                      and 2 mixed infections). Microscopy sensitivity was 72.9%, while cLAMP reached 77.1% with BS DNA and 82.1% with MSC DNA. Specificity remained high (&gt; 99.0%) across methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions\/Significance<\/jats:title>\n                    <jats:p>cLAMP improved the detection of malaria infections from DBS compared with microscopy when combined with column-based DNA extraction in active surveillance activities. While the boil-and-spin method showed performance comparable to microscopy, column-based extraction substantially increased sensitivity, especially for low-density infections identified through active surveillance. These findings support cLAMP as a practical and sensitive complementary tool for malaria surveillance in low-transmission and elimination settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014366","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:46:14Z","timestamp":1791913574000},"page":"e0014366","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Plasmodium vivax and Plasmodium falciparum using colorimetric loop-mediated isothermal amplification from dried blood spots collected in active and passive malaria surveillance in the Peruvian Amazon"],"prefix":"10.1371","volume":"20","author":[{"given":"Oscar","family":"Nolasco","sequence":"first","affiliation":[{"name":"Universidad Peruana Cayetano Heredia","place":["Lima, Peru"],"department":["Laboratorio de Malaria: Par\u00e1sitos y Vectores, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenier\u00eda"]},{"name":"Universidad Nacional Federico Villarreal","place":["Lima, Peru"],"department":["Comunidad de conocimiento GIBBS-UNFV, Facultad de Ciencias Naturales y Matem\u00e1tica"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Malaria Research Group (MaRch), Global Health Institute, Family Medicine and Population Health Department","place":["Antwerp, Belgium"],"department":["Faculty of Medicine and Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Rodr\u00edguez-Ferrucci","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Amazon\u00eda Peruana (UNAP)","place":["Iquitos, Loreto, Peru"],"department":["Facultad de Medicina Humana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionicia","family":"Gamboa","sequence":"additional","affiliation":[{"name":"Universidad Peruana Cayetano Heredia","place":["Lima, Peru"],"department":["Laboratorio de Malaria: Par\u00e1sitos y Vectores, Laboratorios de Investigaci\u00f3n y Desarrollo, Facultad de Ciencias e Ingenier\u00eda"]},{"name":"Universidad Peruana Cayetano Heredia","place":["Lima, Peru"],"department":["Departamento de Ciencias Celulares y Moleculares"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2300-5181","authenticated-orcid":true,"given":"Christopher","family":"Delgado-Ratto","sequence":"additional","affiliation":[{"name":"Malaria Research Group (MaRch), Global Health Institute, Family Medicine and Population Health Department","place":["Antwerp, Belgium"],"department":["Faculty of Medicine and Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"pntd.0014366.ref001","doi-asserted-by":"crossref","DOI":"10.30875\/9789287074560","volume-title":"World Malaria Report 2025","author":"World Health Organization","year":"2025"},{"key":"pntd.0014366.ref002","unstructured":"Centro Nacional de Epidemiolog\u00eda P y C de E. 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