{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:38:53Z","timestamp":1792802333104,"version":"4.5.0"},"reference-count":127,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union Operational Program \u2018\u2018Just Transition\u201d","award":["CZ.10.03.01\/00\/22_003\/0000003"],"award-info":[{"award-number":["CZ.10.03.01\/00\/22_003\/0000003"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["GACR 25-15318S"],"award-info":[{"award-number":["GACR 25-15318S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-74-10012"],"award-info":[{"award-number":["24-74-10012"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      It has been known for decades that long-term cultivation of\n                      <jats:italic>Leishmania in vitro<\/jats:italic>\n                      frequently leads to a loss of virulence, which is attributed to the selective advantage of avirulent subpopulations that outgrow the virulent ones. In the case of\n                      <jats:italic>L. major<\/jats:italic>\n                      , avirulent parasites retained the ability, albeit reduced, to develop nearly normally in sand fly vectors; however, they could not induce lesions in BALB\/c mice. Residual persistence in the inguinal lymph nodes permitted re-isolation and subsequent additional murine passages of these flagellates. While a parasite line obtained after five consecutive passages of avirulent\n                      <jats:italic>Leishmania<\/jats:italic>\n                      in mice was not fully restored to virulence, this line, as well avirulent parasites passaged five times through the sand fly vector\n                      <jats:italic>Phlebotomus duboscqi<\/jats:italic>\n                      developed very efficiently in sand flies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Leishmania major<\/jats:italic>\n                      cell lines with differing capacities for host survival were studied using genomic and transcriptomic approaches. Specifically, we focused on genetic mutations, gene copy number variation, differential gene expression, and differences in kinetoplast DNA, including RNA editing and minicircle repertoire.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>While genetic mutations contributed little to differences in host survival, changes in the gene copy number were correlated with alterations to avirulence. Survival capacity strongly correlated with gene expression patterns. Avirulent parasites showed increased abundance of ribosomal and translation-related transcripts compared with lines capable of persistent survival, suggesting selective pressure to restrict translational capacity in hosts. Most interestingly, the relative abundance of kinetoplast DNA minicircle classes, encoding guide RNAs, was altered during culture but reverted to the virulent pattern following mouse and sand fly passage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our results indicate that at the DNA and mRNA levels,\n                      <jats:italic>L. major<\/jats:italic>\n                      survival in the insect vector or mammalian host is primarily driven by adaptive regulation of gene expression rather than fixed genetic changes. Modulation of translational capacity and host-specific expression programs appear central to parasite persistence, highlighting flexible cellular strategies that support survival in natural transmission cycles.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014387","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:29:18Z","timestamp":1780075758000},"page":"e0014387","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Leishmania major virulence attenuation in vitro: An old conundrum revisited in the omics era"],"prefix":"10.1371","volume":"20","author":[{"given":"Evgeny 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I. Lectin-mediated agglutination","volume":"76","author":"RL Jacobson","year":"1982","journal-title":"Ann Trop Med Parasitol"},{"issue":"6","key":"pntd.0014387.ref013","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1080\/00034983.1999.11813463","article-title":"Leishmania major: effect of repeated passages through sandfly vectors or murine hosts","volume":"93","author":"J S\u00e1dlov\u00e1","year":"1999","journal-title":"Ann Trop Med Parasitol"},{"key":"pntd.0014387.ref014","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.3389\/fimmu.2019.01083","article-title":"Novel Loci Controlling Parasite Load in Organs of Mice Infected With Leishmania major, Their Interactions and Sex Influence","volume":"10","author":"T Kobets","year":"2019","journal-title":"Front Immunol"},{"issue":"3","key":"pntd.0014387.ref015","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1080\/00034983.1997.11813140","article-title":"Development of different Leishmania major strains in the 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FastQC: a quality control tool for high throughput sequence data. 2023."},{"issue":"2","key":"pntd.0014387.ref030","doi-asserted-by":"crossref","DOI":"10.1002\/imt2.107","article-title":"Ultrafast one-pass FASTQ data preprocessing, quality control, and deduplication using fastp","volume":"2","author":"S Chen","year":"2023","journal-title":"Imeta"},{"key":"pntd.0014387.ref031","doi-asserted-by":"crossref","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes de novo assembler","volume":"70","author":"A Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinformatics"},{"key":"pntd.0014387.ref032","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae987","article-title":"OrthoDB and BUSCO update: annotation of orthologs with wider sampling of genomes","volume":"53","author":"F Tegenfeldt","year":"2025","journal-title":"Nucleic Acids 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Garrison","year":"2012"},{"key":"pntd.0014387.ref037","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1467-985X.2010.00676_9.x","article-title":"ggplot2: elegant graphics for data analysis","volume":"174","author":"C Ginestet","year":"2011","journal-title":"J R Stat Soc"},{"issue":"1","key":"pntd.0014387.ref038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011058","article-title":"TriTrypDB: An integrated functional genomics resource for kinetoplastida","volume":"17","author":"A Shanmugasundram","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0014387.ref039","doi-asserted-by":"crossref","DOI":"10.1002\/0471250953.bi1112s47","article-title":"BEDTools: The Swiss-Army Tool for Genome Feature Analysis","volume":"47","author":"AR Quinlan","year":"2014","journal-title":"Curr Protoc Bioinformatics"},{"issue":"18","key":"pntd.0014387.ref040","doi-asserted-by":"crossref","DOI":"10.1093\/bioinformatics\/bts378","article-title":"DELLY: structural 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Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"pntd.0014387.ref053","doi-asserted-by":"crossref","DOI":"10.1128\/msphere.00335-22","article-title":"Elimination of LRVs Elicits Different Responses in Leishmania spp","volume":"7","author":"A Saura","year":"2022","journal-title":"mSphere"},{"issue":"12","key":"pntd.0014387.ref054","doi-asserted-by":"crossref","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-mapper v2: Functional Annotation, Orthology Assignments, and Domain Prediction at the Metagenomic Scale","volume":"38","author":"CP Cantalapiedra","year":"2021","journal-title":"Mol Biol Evol"},{"issue":"1","key":"pntd.0014387.ref055","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1010859","article-title":"Deciphering the RRM-RNA recognition code: A computational analysis","volume":"19","author":"J Roca-Mart\u00ednez","year":"2023","journal-title":"PLoS Comput 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