{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:52:03Z","timestamp":1785495123034,"version":"3.56.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014388","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000}}],"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Foreign and European Affairs, Defence, Development Cooperation and Foreign Trade","award":["PaReCIDS III"],"award-info":[{"award-number":["PaReCIDS III"]}]},{"name":"Ministry of Foreign and European Affairs, Defence, Development Cooperation and Foreign Trade","award":["PaReCIDS III"],"award-info":[{"award-number":["PaReCIDS III"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Despite the widespread implementation of water, sanitation and hygiene (WASH) program over the last two decades, Lao People\u2019s Democratic Republic (Lao PDR) remains highly endemic for intestinal helminth infections. We assessed the intestinal helminth infections burden and the link with WASH levels in an area with WASH interventions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A cross-sectional study was conducted across 81 villages in Khammouane province, Lao PDR, where WASH interventions were implemented. Villages and 30 participants (aged 18\u201387 years) per village were randomly selected. Demographics, individual and household data, including WASH-related factors were collected. Stool samples were examined for intestinal helminth infections using formalin-ethyl acetate concentration technique (FECT). Logistic regression analysis was performed to identify risks associated with intestinal helminth infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 1530 participants were included. Multi-helminth species infection was very common, e.g., 30.1% had a double infection.\n                      <jats:italic>Opisthorchis viverrini<\/jats:italic>\n                      was most prevalent (56.9%), followed by hookworm (54.7%), minute intestinal flukes (22.7%), and\n                      <jats:italic>Trichostrongylus<\/jats:italic>\n                      spp. (10.7%). Overall, 56.1% and 44.4% had access to safely management water services and sanitation facilities, respectively, and 17.7% practiced open defecation. Improved WASH levels were not associated with reduced intestinal helminth infections. District of residence, age, gender and raw food consumption predicted helminth infections. E.g.,\n                      <jats:italic>O. viverrini<\/jats:italic>\n                      infection was associated with residency in Mahaxai district (Mahaxai, aOR = 14.0; 95% CI\u2009=\u20099.5-20.6; p\u2009&lt;\u20090.001 and Bulapha, aOR = 3.0; 95% CI\u2009=\u20092.0-4.4; p\u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings indicate a high intestinal helminth infections burden in this province, with several risk factors such as location, age, gender and consumption of raw food predicting helminth infection. Improved WASH levels are not associated with reduces prevalence of intestinal helminth infection in this cross-sectional study.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0014388","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:47:41Z","timestamp":1780336061000},"page":"e0014388","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["High infection risk of intestinal helminths despite WASH interventions: A cross-sectional study in Khammouane province, Lao 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