{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:27:26Z","timestamp":1786991246652,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0014630","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000}}],"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"World Mosquito Program","award":["CONTRATO N\u00b0 WOLBACHIA-2025-1981"],"award-info":[{"award-number":["CONTRATO N\u00b0 WOLBACHIA-2025-1981"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    A promising sustainable strategy for reducing arbovirus transmission by the mosquito vector\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    is the deployment of individuals transinfected with\n                    <jats:italic>Wolbachia pipientis<\/jats:italic>\n                    , a bacterium that inhibits replication of viruses such as dengue and is being used in population replacement programs aimed at blocking\n                    <jats:italic>Aedes<\/jats:italic>\n                    -borne virus transmission. Between 2017 and 2022, the World Mosquito Program released\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    infected with the\n                    <jats:italic>w<\/jats:italic>\n                    Mel\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strain throughout Medell\u00edn, Colombia. Long-term\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    surveillance following population replacement requires approaches that balance accuracy and cost while providing reliable information regarding infection persistence in targeted populations. Therefore, we assessed current\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    prevalence in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    , surveying five comunas (geographic administrative units) of Medell\u00edn spanning ~32.66 km, using ovitrap collections and field sampling of adult mosquitoes and assessed\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infection using conventional PCR and quantitative PCR (qPCR) in pooled and individual samples. We found that\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infection remains widespread across the surveyed areas, with estimated infection frequencies ranging from ~60% to near fixation depending on sampling strategy and molecular detection method. Screening pooled adults emerging from ovitraps using conventional PCR provided a cost-effective method for confirming\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    persistence across sites, whereas qPCR of individual field-captured adults provided more sensitive estimates of infection prevalence. Our results suggest that\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    is broadly established in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    populations in the surveyed areas and demonstrate practical approaches for the long-term surveillance of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -based vector control programs.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0014630","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T17:36:06Z","timestamp":1786383366000},"page":"e0014630","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Wolbachia prevalence in Aedes aegypti across five comunas of Medell\u00edn, Colombia: Implications for post-release surveillance"],"prefix":"10.1371","volume":"20","author":[{"given":"Luisa M.","family":"Barrientos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego F.","family":"Rincon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7278-0646","authenticated-orcid":true,"given":"Frank W.","family":"Avila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalina","family":"Alfonso-Parra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"pntd.0014630.ref001","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0140-6736(14)60572-9","article-title":"Dengue","volume":"385","author":"MG 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