{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:00:14Z","timestamp":1791068414627,"version":"4.1.0"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2008,6,25]],"date-time":"2008-06-25T00:00:00Z","timestamp":1214352000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0002487","type":"journal-article","created":{"date-parts":[[2008,6,24]],"date-time":"2008-06-24T21:54:08Z","timestamp":1214344448000},"page":"e2487","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":61,"title":["A Francisella tularensis Schu S4 Purine Auxotroph Is Highly Attenuated in Mice but Offers Limited Protection against Homologous Intranasal Challenge"],"prefix":"10.1371","volume":"3","author":[{"given":"Roger D.","family":"Pechous","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis R.","family":"McCarthy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nrusingh P.","family":"Mohapatra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa","family":"Soni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee M.","family":"Penoske","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nita H.","family":"Salzman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dara W.","family":"Frank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Gunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Zahrt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2008,6,25]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.2460\/javma.2003.222.725","article-title":"Tularemia.","volume":"222","author":"KA Feldman","year":"2003","journal-title":"J Am Vet Med Assoc"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1001\/archinte.1961.03620050068007","article-title":"Tularemia vaccine study. II. Respiratory challenge.","volume":"107","author":"S Saslaw","year":"1961","journal-title":"Arch Intern Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1001\/archinte.1961.03620050055006","article-title":"Tularemia vaccine study. I. Intracutaneous challenge.","volume":"107","author":"S Saslaw","year":"1961","journal-title":"Arch Intern Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.2105\/AJPH.32.10.1131","article-title":"Vaccine Prophylaxis against Tularemia in Man.","volume":"32","author":"L Foshay","year":"1942","journal-title":"Am J Public Health Nations Health"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"621","DOI":"10.7326\/0003-4819-50-3-621","article-title":"Laboratory-acquired tularemia in vaccinated individuals: a report of 62 cases.","volume":"50","author":"TE Van Metre Jr","year":"1959","journal-title":"Ann Intern Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1574-695X.2007.00219.x","article-title":"Francisella tularensis vaccines.","volume":"49","author":"KF Griffin","year":"2007","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0882-4010(03)00046-9","article-title":"Experimental tularemia in mice challenged by aerosol or intradermally with virulent strains of Francisella tularensis: bacteriologic and histopathologic studies.","volume":"34","author":"JW Conlan","year":"2003","journal-title":"Microb Pathog"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1128\/MMBR.25.3.262-267.1961","article-title":"Aerosol Infection of Man with Pasteurella Tularensis.","volume":"25","author":"FR McCrumb","year":"1961","journal-title":"Bacteriol Rev"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"S61","DOI":"10.1086\/518150","article-title":"From rabbits to humans: the contributions of Dr. Theodore E. Woodward to tularemia research.","volume":"45","author":"AS Cross","year":"2007","journal-title":"Clin Infect Dis"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1128\/MMBR.30.3.532-538.1966","article-title":"Aerogenic immunization of man with live Tularemia vaccine.","volume":"30","author":"RB Hornick","year":"1966","journal-title":"Bacteriol Rev"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1128\/IAI.73.5.2644-2654.2005","article-title":"Intranasal vaccination induces protective immunity against intranasal infection with virulent Francisella tularensis biovar A.","volume":"73","author":"TH Wu","year":"2005","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"8345","DOI":"10.1128\/IAI.73.12.8345-8352.2005","article-title":"A mutant of Francisella tularensis strain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine.","volume":"73","author":"S Twine","year":"2005","journal-title":"Infect Immun"},{"key":"ref13","article-title":"Francisella tularensis tularensis Schu S4 Disulfide bond formation protein B, (DsbB), but not an RND-type Efflux pump, is required for virulence.","author":"A Qin","year":"2008","journal-title":"Infect Immun [Epub ahead of print]"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1128\/IAI.01241-06","article-title":"The immunologically distinct O antigens from Francisella tularensis subspecies tularensis and Francisella novicida are both virulence determinants and protective antigens.","volume":"75","author":"RM Thomas","year":"2007","journal-title":"Infect Immun"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1099\/mic.0.29121-0","article-title":"Genetic tools for highly pathogenic Francisella tularensis subsp. tularensis.","volume":"152","author":"ED LoVullo","year":"2006","journal-title":"Microbiology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"4452","DOI":"10.1128\/IAI.00666-06","article-title":"Construction and characterization of an attenuated purine auxotroph in a Francisella tularensis live vaccine strain.","volume":"74","author":"R Pechous","year":"2006","journal-title":"Infect Immun"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"7511","DOI":"10.1128\/AEM.70.12.7511-7519.2004","article-title":"Construction and characterization of a highly efficient Francisella shuttle plasmid.","volume":"70","author":"TM Maier","year":"2004","journal-title":"Appl Environ Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"11470","DOI":"10.1073\/pnas.88.24.11470","article-title":"Intracellular replication is essential for the virulence of Salmonella typhimurium.","volume":"88","author":"KY Leung","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref19","first-page":"714","article-title":"The effects of biochemical mutation on the virulence of Bacterium typhosum: the virulence of mutants.","volume":"31","author":"GA Bacon","year":"1950","journal-title":"Br J Exp Pathol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1128\/IAI.01254-06","article-title":"Francisella tularensis replicates within alveolar type II epithelial cells in vitro and in vivo following inhalation.","volume":"75","author":"JD Hall","year":"2007","journal-title":"Infect Immun"},{"key":"ref21","doi-asserted-by":"crossref","DOI":"10.1007\/b97377","article-title":"Survival Analysis: Techniques for Censored and Truncated Data","author":"JP Klein","year":"2003"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1111\/j.1462-5822.2007.01022.x","article-title":"Activation of the inflammasome upon Francisella tularensis infection: interplay of innate immune pathways and virulence factors.","volume":"9","author":"T Henry","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1073\/pnas.0504271103","article-title":"Internalization and phagosome escape required for Francisella to induce human monocyte IL-1beta processing and release.","volume":"103","author":"MA Gavrilin","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"4002","DOI":"10.1128\/IAI.00257-06","article-title":"Identification, recombinant expression, immunolocalization in macrophages, and T-cell responsiveness of the major extracellular proteins of Francisella tularensis.","volume":"74","author":"BY Lee","year":"2006","journal-title":"Infect Immun"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1016\/S0264-410X(03)00386-4","article-title":"Tularemia in BALB\/c and C57BL\/6 mice vaccinated with Francisella tularensis LVS and challenged intradermally, or by aerosol with virulent isolates of the pathogen: protection varies depending on pathogen virulence, route of exposure, and host genetic background.","volume":"21","author":"W Chen","year":"2003","journal-title":"Vaccine"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1099\/jmm.0.46313-0","article-title":"Transcriptional profiling of host responses in mouse lungs following aerosol infection with type A Francisella tularensis.","volume":"55","author":"H Andersson","year":"2006","journal-title":"J Med Microbiol"},{"key":"ref27","article-title":"SAS 9.1 online documentation."}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0002487","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T15:59:29Z","timestamp":1588780769000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0002487"}},"subtitle":[],"editor":[{"given":"Niyaz","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2008,6,25]]},"references-count":27,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2008,6,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0002487","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6,25]]}}}