{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T20:36:31Z","timestamp":1759091791746},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2008,7,16]],"date-time":"2008-07-16T00:00:00Z","timestamp":1216166400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"DOI":"10.1371\/journal.pone.0002694","type":"journal-article","created":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T21:59:11Z","timestamp":1216159151000},"page":"e2694","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Modulating Vesicle Priming Reveals that Vesicle Immobilization Is Necessary but not Sufficient for Fusion-Competence"],"prefix":"10.1371","volume":"3","author":[{"given":"Ofer","family":"Yizhar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uri","family":"Ashery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2008,7,16]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s00441-006-0243-z","article-title":"Vesicle pools, docking, priming, and release.","volume":"326","author":"U Becherer","year":"2006","journal-title":"Cell Tissue Res"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0896-6273(00)00131-8","article-title":"Dissection of Three Ca2+-Dependent Steps Leading to Secretion in Chromaffin Cells from Mouse Adrenal Slices.","volume":"28","author":"T Voets","year":"2000","journal-title":"Neuron"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/S0896-6273(00)80147-6","article-title":"Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules.","volume":"16","author":"KD Gillis","year":"1996","journal-title":"Neuron"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/S0896-6273(00)80504-8","article-title":"Cytosolic Ca2+ acts by two separate pathways to modulate the supply of release-competent vesicles in chromaffin cells.","volume":"20","author":"C Smith","year":"1998","journal-title":"Neuron"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/S0896-6273(00)80146-4","article-title":"Definition of the readily releasable pool of vesicles at hippocampal synapses.","volume":"16","author":"C Rosenmund","year":"1996","journal-title":"Neuron"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1073\/pnas.92.3.846","article-title":"Estimates for the pool size of releasable quanta at a single central synapse and for the time required to refill the pool.","volume":"92","author":"CF Stevens","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.1093\/emboj\/19.14.3586","article-title":"Munc13-1 acts as a priming factor for large dense-core vesicles in bovine chromaffin cells.","volume":"19","author":"U Ashery","year":"2000","journal-title":"EMBO J"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/S0896-6273(00)80812-0","article-title":"Mechanisms underlying phasic and sustained secretion in chromaffin cells from mouse adrenal slices.","volume":"23","author":"T Voets","year":"1999","journal-title":"Neuron"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/642","article-title":"Multiple kinetic components of exocytosis distinguished by neurotoxin sensitivity.","volume":"1","author":"T Xu","year":"1998","journal-title":"Nat Neurosci"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1038\/sj.emboj.7601003","article-title":"Sequential N- to C-terminal SNARE complex assembly drives priming and fusion of secretory vesicles.","volume":"25","author":"JB Sorensen","year":"2006","journal-title":"Embo J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S0092-8674(00)81669-4","article-title":"Inhibition of SNARE complex assembly differentially affects kinetic components of exocytosis.","volume":"99","author":"T Xu","year":"1999","journal-title":"Cell"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"10354","DOI":"10.1021\/bi980542h","article-title":"Identification of a minimal core of the synaptic SNARE complex sufficient for reversible assembly and disassembly.","volume":"37","author":"D Fasshauer","year":"1998","journal-title":"Biochemistry"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"27703","DOI":"10.1074\/jbc.C000237200","article-title":"Assembly of snare core complexes occurs prior to neurotransmitter release to set the readily-releasable pool of synaptic vesicles.","volume":"275","author":"G Lonart","year":"2000","journal-title":"J Biol Chem"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1126\/science.1075375","article-title":"Emerging roles of presynaptic proteins in Ca++-triggered exocytosis.","volume":"298","author":"J Rettig","year":"2002","journal-title":"Science"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1038\/14764","article-title":"Drosophila UNC-13 is essential for synaptic transmission.","volume":"2","author":"B Aravamudan","year":"1999","journal-title":"Nat Neurosci"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1042\/bj3370363","article-title":"Differential expression of two novel Munc13 proteins in rat brain.","volume":"337","author":"I Augustin","year":"1999","journal-title":"Biochem J"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1038\/12160","article-title":"UNC-13 is required for synaptic vesicle fusion in C. elegans.","volume":"2","author":"JE Richmond","year":"1999","journal-title":"Nat Neurosci"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1073\/pnas.0308700100","article-title":"Tomosyn inhibits priming of large dense-core vesicles in a calcium-dependent manner.","volume":"101","author":"O Yizhar","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1083\/jcb.200504055","article-title":"PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter.","volume":"170","author":"T Baba","year":"2005","journal-title":"J Cell Biol"},{"key":"ref20","article-title":"Tomosyn negatively regulates both synaptic transmitter and neuropeptide release at the C. elegans neuromuscular junction.","author":"EO Gracheva","year":"2007","journal-title":"J Physiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.neuron.2006.06.025","article-title":"Antagonistic Regulation of Synaptic Vesicle Priming by Tomosyn and UNC-13.","volume":"51","author":"JM McEwen","year":"2006","journal-title":"Neuron"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/S0896-6273(00)80472-9","article-title":"Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process.","volume":"20","author":"Y Fujita","year":"1998","journal-title":"Neuron"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/nature05635","article-title":"Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism.","volume":"446","author":"DA Hattendorf","year":"2007","journal-title":"Nature"},{"key":"ref24","article-title":"Multiple functional domains are involved in tomosyn regulation of exocytosis.","author":"O Yizhar","year":"2007","journal-title":"J Neurochem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"22887","DOI":"10.1074\/jbc.M701787200","article-title":"Receptor-mediated regulation of tomosyn-syntaxin 1A interactions in bovine adrenal chromaffin cells.","volume":"282","author":"SE Gladycheva","year":"2007","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"31159","DOI":"10.1074\/jbc.M305500200","article-title":"The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis.","volume":"278","author":"K Hatsuzawa","year":"2003","journal-title":"J Biol Chem"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"35093","DOI":"10.1074\/jbc.M304261200","article-title":"Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation.","volume":"278","author":"CH Widberg","year":"2003","journal-title":"J Biol Chem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"574","DOI":"10.2337\/diabetes.55.03.06.db05-0015","article-title":"Tomosyn is expressed in beta-cells and negatively regulates insulin exocytosis.","volume":"55","author":"W Zhang","year":"2006","journal-title":"Diabetes"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1083\/jcb.200405002","article-title":"Regulation of SNAREs by tomosyn and ROCK: implication in extension and retraction of neurites.","volume":"166","author":"T Sakisaka","year":"2004","journal-title":"J Cell Biol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1371\/journal.pbio.0040261","article-title":"Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans.","volume":"4","author":"EO Gracheva","year":"2006","journal-title":"PLos Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1083\/jcb.139.7.1709","article-title":"Ultrastructural organization of bovine chromaffin cell cortex-analysis by cryofixation and morphometry of aspects pertinent to exocytosis.","volume":"139","author":"H Plattner","year":"1997","journal-title":"J Cell Biol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1091\/mbc.E05-10-0938","article-title":"Motion matters: secretory granule motion adjacent to the plasma membrane and exocytosis.","volume":"17","author":"MW Allersma","year":"2006","journal-title":"Mol Biol Cell"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1083\/jcb.153.1.177","article-title":"Restriction of secretory granule motion near the plasma membrane of chromaffin cells.","volume":"153","author":"LM Johns","year":"2001","journal-title":"J Cell Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s002490050253","article-title":"Tracking chromaffin granules on their way through the actin cortex.","volume":"29","author":"M Oheim","year":"2000","journal-title":"Eur Biophys J"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1038\/35022500","article-title":"Transport, capture and exocytosis of single synaptic vesicles at active zones.","volume":"406","author":"D Zenisek","year":"2000","journal-title":"Nature"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1146\/annurev.bb.13.060184.001335","article-title":"Total internal reflection fluorescence.","volume":"13","author":"D Axelrod","year":"1984","journal-title":"Annu Rev Biophys Bioeng"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"3725","DOI":"10.1038\/sj.emboj.7601256","article-title":"Dissecting docking and tethering of secretory vesicles at the target membrane.","volume":"25","author":"RF Toonen","year":"2006","journal-title":"Embo J"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1523\/JNEUROSCI.4714-06.2007","article-title":"Primed vesicles can be distinguished from docked vesicles by analyzing their mobility.","volume":"27","author":"S Nofal","year":"2007","journal-title":"J Neurosci"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"e126","DOI":"10.1371\/journal.pone.0000126","article-title":"Docking of secretory vesicles is syntaxin dependent.","volume":"1","author":"H de Wit","year":"2006","journal-title":"PLoS ONE"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e198","DOI":"10.1371\/journal.pbio.0050198","article-title":"Open syntaxin docks synaptic vesicles.","volume":"5","author":"M Hammarlund","year":"2007","journal-title":"PLoS Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/nature01389","article-title":"Functional and spatial segregation of secretory vesicle pools according to vesicle age.","volume":"422","author":"RR Duncan","year":"2003","journal-title":"Nature"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/nbt0102-87","article-title":"A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.","volume":"20","author":"T Nagai","year":"2002","journal-title":"Nat Biotechnol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/S0896-6273(00)80325-6","article-title":"Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy.","volume":"18","author":"T Lang","year":"1997","journal-title":"Neuron"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1016\/S0006-3495(99)77382-0","article-title":"Tracking single secretory granules in live chromaffin cells by evanescent-field fluorescence microscopy.","volume":"76","author":"JA Steyer","year":"1999","journal-title":"Biophys J"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/41329","article-title":"Transport, docking and exocytosis of single secretory granules in live chromaffin cells.","volume":"388","author":"JA Steyer","year":"1997","journal-title":"Nature"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/28190","article-title":"Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins.","volume":"394","author":"G Miesenbock","year":"1998","journal-title":"Nature"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.1016\/S0006-3495(94)80744-1","article-title":"Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+.","volume":"67","author":"C Heinemann","year":"1994","journal-title":"Biophys J"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s002490050188","article-title":"Multiple stimulation-dependent processes regulate the size of the releasable pool of vesicles.","volume":"28","author":"M Oheim","year":"1999","journal-title":"Eur Biophys J"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"2912","DOI":"10.1242\/jcs.03037","article-title":"Tomosyn-1 is involved in a post-docking event required for pancreatic {beta}-cell exocytosis.","volume":"119","author":"S Cheviet","year":"2006","journal-title":"J Cell Sci"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"e505","DOI":"10.1371\/journal.pone.0000505","article-title":"Quantifying exocytosis by combination of membrane capacitance measurements and total internal reflection fluorescence microscopy in chromaffin cells.","volume":"2","author":"U Becherer","year":"2007","journal-title":"PLoS ONE"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.neuroscience.2006.08.014","article-title":"Munc18-1 phosphorylation by protein kinase C potentiates vesicle pool replenishment in bovine chromaffin cells.","volume":"143","author":"U Nili","year":"2006","journal-title":"Neuroscience"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0896-6273(01)00391-9","article-title":"Munc18-1 promotes large dense-core vesicle docking.","volume":"31","author":"T Voets","year":"2001","journal-title":"Neuron"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/nrm2002","article-title":"SNAREs\u2013engines for membrane fusion.","volume":"7","author":"R Jahn","year":"2006","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.tibs.2005.05.002","article-title":"SNARE complexes and neuroexocytosis: how many, how close?","volume":"30","author":"C Montecucco","year":"2005","journal-title":"Trends Biochem Sci"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0896-6273(00)80559-0","article-title":"Tomosyn binds t-SNARE proteins via a VAMP-like coiled coil.","volume":"21","author":"ES Masuda","year":"1998","journal-title":"Neuron"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1038\/35085583","article-title":"An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming.","volume":"412","author":"JE Richmond","year":"2001","journal-title":"Nature"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0171-9335(99)80017-X","article-title":"An efficient method for infection of adrenal chromaffin cells using the Semliki Forest virus gene expression system.","volume":"78","author":"U Ashery","year":"1999","journal-title":"Eur J Cell Biol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1016\/S0006-3495(00)76828-7","article-title":"Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.","volume":"78","author":"T Lang","year":"2000","journal-title":"Biophys J"},{"key":"ref59","article-title":"Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.","author":"VE Degtyar","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1042\/bj20021090","article-title":"The role of myosin in vesicle transport during bovine chromaffin cell secretion.","volume":"368","author":"P Neco","year":"2002","journal-title":"Biochem J"}],"container-title":["PLoS ONE"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pone.0002694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T04:46:10Z","timestamp":1631508370000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0002694"}},"subtitle":[],"editor":[{"given":"Richard","family":"Steinhardt","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2008,7,16]]},"references-count":60,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2008,7,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0002694","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,16]]}}}